FastQCFastQC Report
Wed 17 Sep 2014
G50_MOV_A4_L4_AGGCAGAA-AAGGAGTA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_MOV_A4_L4_AGGCAGAA-AAGGAGTA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998757
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC801.0806367E-535.6097261
AGGGGTG1404.5656634E-1033.9208265
TAAGGGT1001.3012868E-633.242414
ACTGACC2000.030.8756858
CAGGGGT1901.2732926E-1129.9931494
CTAAGCG804.4992362E-429.6807173
GCCTTAG804.5045192E-429.6747721
GGTGTGC1652.6466296E-928.7885178
CTGTCCC1503.3365723E-828.4992059
GTCCTTA700.00840040127.136666
GTCGCCG28950.026.59422594-95
TCGCCGT16350.026.16047794-95
CTGTGCA2207.6397555E-1125.9083659
GTTCTAG3850.025.8979851
TACCGAG1151.2813948E-424.7831577
ACCTCGT1751.4854231E-724.4291118
TACACCG1751.4890247E-724.4229935
GGTCGCC39000.024.24986894-95
CGCCGTA9250.023.89087994-95
TAAAGGG1401.7823235E-523.7445774
Produced by FastQC (version 0.11.2)