FastQCFastQC Report
Wed 17 Sep 2014
G50_MOV_A4_L4_AGGCAGAA-AAGGAGTA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_MOV_A4_L4_AGGCAGAA-AAGGAGTA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998757
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCG554.9632443E-543.1812255
CGTAGCC500.00161502938.0032843
GTCTTAG905.653055E-736.9809651
AGGGGTG1651.8189894E-1234.544985
AGACTGG1404.5656634E-1033.9281046
GTGTACG753.060373E-431.6979711
GGGTGTG1351.1970769E-831.6662317
ATACCGT1509.549694E-1031.6662316
TACACCG1551.3569661E-930.6447415
CAGGGGT2300.028.912654
TAGAGCG1205.3813237E-627.7079547
GTCCTAT1056.85865E-527.169691
CCAGGGG1056.900882E-527.1452033
TAGGGTG1056.905588E-527.1424875
CCGAGGG700.00839161427.1424859
TAAGGTG1405.805523E-727.1424855
TCTATAC1606.234768E-826.7210583
CTGACCA1606.2409526E-826.7183829
CCTCTAG1257.3331285E-626.6262971
CCATCCG1257.3904157E-626.5996389
Produced by FastQC (version 0.11.2)