FastQCFastQC Report
Wed 17 Sep 2014
G50_MOV_A2_L31_TAAGGCGA-AAGGAGTA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_MOV_A2_L31_TAAGGCGA-AAGGAGTA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299691
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT6450.021.3477521
TAGACCG4650.020.424135
TACACCG9150.019.7210035
TAGGGTG4650.019.4029245
TATACCG8350.019.3356575
TACACTG17700.019.316385
CTAGCAC4950.018.2269883
CGTACAC6900.017.8933143
GTACTAG6650.017.8497541
CTGTCCC7750.017.7711479
GGTCGCC28750.017.76698594-95
CTACACA15650.017.2952584
CTACACT16500.017.2676734
TCTATAC11450.017.0037573
CGTAGAC8400.016.9593223
CTATACT10950.016.9128574
GCTACAC7750.016.5435453
TCTACAC17900.016.44773
CTAATAC11850.016.429793
TATACAG13950.016.3393045
Produced by FastQC (version 0.11.2)