FastQCFastQC Report
Wed 17 Sep 2014
G50_MOV_A2_L31_TAAGGCGA-AAGGAGTA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_MOV_A2_L31_TAAGGCGA-AAGGAGTA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299691
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC6700.021.9774388
GTGTAGG13550.021.7486441
TACACTG17200.019.8835625
TCTATAC9600.019.2975983
CTATACT11500.018.9998474
TATACCG8100.018.7652845
CCTACAC10950.018.6536673
GTATATA20950.017.9235041
GTATTAG11400.017.5115361
CTAGACG4353.6379788E-1217.4711254
TAGACCG4353.6379788E-1217.4711255
GTGTAGA20150.017.219781
GACTGTG9400.017.1807147
GTCCTAC5300.017.0395281
CCTATAC10900.016.996053
ACTGCGC6450.016.9378498
GACCGTG5100.016.7645727
ACAGTCC8000.016.6248688
GTCTTAG7800.016.4531461
TACACCG8700.016.379185
Produced by FastQC (version 0.11.2)