FastQCFastQC Report
Wed 17 Sep 2014
G50_MOV_A1_L34_AAGAGGCA-ACTGCATA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_MOV_A1_L34_AAGAGGCA-ACTGCATA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4187463
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG12050.023.2480755
TACACTG20750.021.2807485
TATACCG9850.020.2458065
TATACAG15950.020.2427855
CTACACT17900.019.8943734
CTAGCAC6350.019.4411353
CTTACAC11750.018.58843
ACCGTGC10300.018.442848
GTATAAG12350.017.6838421
TACACAG16750.017.5751445
CTAACAC11450.017.4166983
ACACCGT12600.017.3346276
GTCTTAG9200.017.029891
CTATACT12350.016.9163934
CTGTCCC10600.016.5770059
GTAGCAC13050.016.372843
GTATATA23800.016.3577161
CCTACAC14900.016.2519683
TAGACAG11150.016.1819485
GTATTAG12150.016.0211371
Produced by FastQC (version 0.11.2)