FastQCFastQC Report
Wed 17 Sep 2014
G50_MOV_A1_L34_AAGAGGCA-ACTGCATA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_MOV_A1_L34_AAGAGGCA-ACTGCATA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4187463
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT13100.021.7554874
GTGTAGC11250.021.5495661
ACTGTCC12750.020.4899738
TACACAG16050.020.4203365
TACACTG22150.020.1577935
TCTATAC13900.019.1369383
GTGTAAG12250.019.0143241
GTGTTAG12900.018.7932261
TCTACAC22150.018.0137793
ACAGTGC17900.017.7791358
CTACACT17450.017.6932164
CTACACA19350.017.6742254
TAGACTG10800.017.59245
CCTATAC13250.017.5662633
GTCCTAC5200.017.3688531
GTATATA26000.017.3688531
GTAGCAC13200.017.2729513
CCTACAC14900.017.2149893
GTCCTAA6100.017.1440621
TACACCG12800.017.0701275
Produced by FastQC (version 0.11.2)