FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L96_CGAGGCTG-ACTGCATA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L96_CGAGGCTG-ACTGCATA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2929227
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACA4000.022.5555294
CCTACAC11950.021.855173
GTCTAGA3900.020.6980251
GTCCTAC4600.020.6450881
TATAGGG6500.020.4552172
GTATTAT10800.020.224541
TACACTG7300.020.1655
TACACCG19650.019.5741025
GTAGGAC4400.019.4258163
TACACAG6450.019.141375
TATACCG9450.019.094635
CCTAGCA2251.6743779E-618.994132
ATCCCGT9650.018.6992036
ATAGGGT5450.018.2970963
TCTATAC6750.018.2906443
TAGGACC2352.537612E-618.1858674
CCTAGGG2101.9626928E-518.0896472
CTAGCAC2453.7768277E-617.4435883
CTAGACA3059.321957E-817.1258544
GTTCTAG4751.8189894E-1216.9941671
Produced by FastQC (version 0.11.2)