FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L96_CGAGGCTG-ACTGCATA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L96_CGAGGCTG-ACTGCATA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2929227
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAG5950.024.747625
GTGTAGG10450.024.5657221
TATACCG8300.022.3190235
TAGGACT3251.8189894E-1221.9228294
CCTACAC12650.021.0292243
ATAGGGT5450.020.9189783
ACCCTAT7050.020.9037721
TACACTG8450.020.2364585
CTAACAC4000.020.1889953
CTACACA6500.019.7305454
GTATAGG7000.019.6948221
TCTATAC5800.019.6566283
TACACCG18050.019.4734635
CTATACT4900.019.3875374
TATAGGG8100.019.3546072
TAGGGTT7650.019.2481484
GATAGGG6050.018.8456572
CTATACC9200.018.5867464
GTATACG4650.018.4022791
GCCCTAT5250.018.1101821
Produced by FastQC (version 0.11.2)