FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L95_GCTACGCT-ACTGCATA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L95_GCTACGCT-ACTGCATA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815137
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGGT1951.5725163E-824.356844
GTGTAAG2950.024.1484321
ATAGGAC2903.6379788E-1222.9290263
CTATACT3400.022.3509834
TAGGGCA2405.9390004E-921.7689254
TATATAC5350.021.3065433
TAGACTG3900.020.7033145
TACACTC3900.020.7033145
GTATTAA5350.020.4170841
TACACTG5500.019.8618985
GTCTAGA3353.45608E-1119.8473661
TTAGACT3850.019.7385314
CTACACT5400.019.3501574
GTATAGA3951.8189894E-1219.237231
TACACAG4001.8189894E-1218.9983375
GTGCTAA3254.8930815E-1018.9967651
TACACCG10600.018.8191075
GTTCTAG4650.018.3839661
CTTAGGG1300.00756859318.267633
TAGGACA4201.8189894E-1218.0936534
Produced by FastQC (version 0.11.2)