FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L95_GCTACGCT-ACTGCATA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L95_GCTACGCT-ACTGCATA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815137
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT3150.030.158314
GTGCTAG1802.0918378E-1029.0529841
GCTATAC2700.028.1508583
GGACAGT3850.023.4412336
TACACAG3650.023.4243325
GTATTAC4100.023.1908531
GCTACAC6750.022.5206873
GTGTACG6400.021.5421261
TACACTG4650.021.4513155
CTAACAC3600.021.1131443
TATACCG3401.8189894E-1220.9555915
GTGTATG6150.020.0987381
TCTATAC3800.020.0019253
GTCTAGA3102.564775E-1019.9366531
CCTAGTA1456.1427936E-419.6576062
TACACCG9700.019.0976735
GTGTATA3254.820322E-1019.0164991
TCCTAGA2251.6667418E-619.0023542
TAACACC3759.094947E-1218.9997374
ACTGTCC4250.018.9997358
Produced by FastQC (version 0.11.2)