##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename G140_A6_L93_CAGAGAGG-ACTGCATA_L006_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 361727 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21132235083364 33.0 33.0 33.0 33.0 33.0 2 32.211844844316296 33.0 33.0 33.0 33.0 33.0 3 32.20661438045819 33.0 33.0 33.0 33.0 33.0 4 36.060111631147244 37.0 37.0 37.0 37.0 37.0 5 35.987689611226145 37.0 37.0 37.0 33.0 37.0 6 36.027014848214264 37.0 37.0 37.0 37.0 37.0 7 35.97454433868636 37.0 37.0 37.0 33.0 37.0 8 35.97770694474009 37.0 37.0 37.0 33.0 37.0 9 36.021557694062096 37.0 37.0 37.0 37.0 37.0 10-11 35.968502489446465 37.0 37.0 37.0 35.0 37.0 12-13 35.919694963328695 37.0 37.0 37.0 33.0 37.0 14-15 37.96305501109953 40.0 37.0 40.0 33.0 40.0 16-17 37.87697628321911 40.0 37.0 40.0 33.0 40.0 18-19 37.78499254962997 40.0 37.0 40.0 33.0 40.0 20-21 37.69716250100214 40.0 37.0 40.0 33.0 40.0 22-23 37.565004824080034 40.0 37.0 40.0 33.0 40.0 24-25 37.429311331473734 40.0 37.0 40.0 33.0 40.0 26-27 37.2204936319379 40.0 37.0 40.0 33.0 40.0 28-29 37.06844941074346 40.0 37.0 40.0 33.0 40.0 30-31 36.893857798837246 40.0 37.0 40.0 33.0 40.0 32-33 36.671929383208884 40.0 37.0 40.0 33.0 40.0 34-35 36.64175469345667 40.0 37.0 40.0 33.0 40.0 36-37 36.79165503266275 40.0 37.0 40.0 33.0 40.0 38-39 36.68356523013212 40.0 37.0 40.0 33.0 40.0 40-41 36.48095248626727 40.0 37.0 40.0 33.0 40.0 42-43 36.26513226825755 37.0 37.0 40.0 33.0 40.0 44-45 36.017574026821336 37.0 37.0 40.0 27.0 40.0 46-47 35.82826827966947 37.0 37.0 40.0 27.0 40.0 48-49 35.57473453737211 37.0 37.0 40.0 27.0 40.0 50-51 34.44721571793092 37.0 33.0 38.5 27.0 40.0 52-53 34.65552059978934 37.0 33.0 38.5 27.0 40.0 54-55 35.41651577018027 37.0 35.0 40.0 27.0 40.0 56-57 35.36346471233831 37.0 33.0 40.0 27.0 40.0 58-59 35.16655101775648 37.0 33.0 40.0 27.0 40.0 60-61 34.96866974265123 37.0 33.0 40.0 27.0 40.0 62-63 34.7361864057701 37.0 33.0 37.0 27.0 40.0 64-65 34.50519590741084 37.0 33.0 37.0 27.0 40.0 66-67 34.23984386014868 37.0 33.0 37.0 27.0 40.0 68-69 34.02163510050397 37.0 33.0 37.0 27.0 40.0 70-71 33.79604231920758 37.0 33.0 37.0 27.0 40.0 72-73 33.56464681928637 37.0 33.0 37.0 27.0 37.0 74-75 33.36774694728345 37.0 33.0 37.0 22.0 37.0 76-77 33.12044580581488 37.0 33.0 37.0 22.0 37.0 78-79 32.88945668971351 37.0 33.0 37.0 22.0 37.0 80-81 32.636760595697865 37.0 33.0 37.0 22.0 37.0 82-83 32.399190549779256 37.0 33.0 37.0 22.0 37.0 84-85 32.19037146798553 37.0 33.0 37.0 22.0 37.0 86-87 31.95332529780746 37.0 33.0 37.0 22.0 37.0 88-89 31.72606136672131 33.0 33.0 37.0 18.5 37.0 90-91 31.50499271550092 33.0 33.0 37.0 15.0 37.0 92-93 31.26966054510722 33.0 33.0 37.0 15.0 37.0 94-95 31.005895329903492 33.0 27.0 37.0 15.0 37.0 96-97 30.745390861063733 33.0 27.0 37.0 15.0 37.0 98-99 30.4478681436553 33.0 27.0 37.0 15.0 37.0 100-101 29.442657031407663 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.19086902020727337 2306 2 0.2261300787810896 2306 3 0.17558040423999444 2306 4 0.2294233258679128 2306 5 0.23089047010415698 2306 6 0.42457033262031274 2306 7 0.1697762142675785 2306 8 0.3407553744743339 2306 9 0.14324582933878105 2306 10-11 0.1250162521494289 2306 12-13 0.24323054602891148 2306 14-15 0.2861837542712209 2306 16-17 0.28793887113281613 2306 18-19 0.1065838996387285 2306 20-21 0.17311382423495303 2306 22-23 0.1027415311881299 2306 24-25 -0.015056144050603848 2306 26-27 0.11094766465743078 2306 28-29 -0.25576454540627935 2306 30-31 -0.31271095639134217 2306 32-33 -0.5041583273726431 2306 34-35 -0.15472804899005865 2306 36-37 -0.3406413660633021 2306 38-39 -0.37215775186273703 2306 40-41 -0.3127509594738811 2306 42-43 -0.16412449758215786 2306 44-45 -0.2513070366893828 2306 46-47 -0.45969702560425674 2306 48-49 -0.25802776369918945 2306 50-51 -0.34799679204780176 2306 52-53 -0.5634968919993568 2306 54-55 -0.509663479856961 2306 56-57 -0.4035784633508612 2306 58-59 -0.50300029464848 2306 60-61 -0.48719272156991167 2306 62-63 -0.5270418380840596 2306 64-65 -0.5945817222241132 2306 66-67 -0.3969802097067614 2306 68-69 -0.7315285329709056 2306 70-71 -0.5776135335368409 2306 72-73 -0.5838579601818168 2306 74-75 -0.6331351042542153 2306 76-77 -1.147406710776277 2306 78-79 -0.9940243367686179 2306 80-81 -1.2239961369874592 2306 82-83 -1.336491263956077 2306 84-85 -1.3112888634584898 2306 86-87 -1.4938105410426843 2306 88-89 -1.616958011283078 2306 90-91 -1.3772934657406637 2306 92-93 -1.920446154112117 2306 94-95 -1.7615045259543045 2306 96-97 -1.8888653237725812 2306 98-99 -2.2345247360824203 2306 100-101 -2.209615583756971 2307 1 0.20740270287171825 2307 2 0.09875323784583401 2307 3 0.19828692641060996 2307 4 0.28995089523792217 2307 5 0.2824267330552104 2307 6 -0.10764355329132513 2307 7 0.1682081939428599 2307 8 -0.02927757380084728 2307 9 -0.01393332194540875 2307 10-11 0.3492133860464506 2307 12-13 0.15286096959287931 2307 14-15 0.2964949765331397 2307 16-17 0.2293316304516253 2307 18-19 0.31145369440670123 2307 20-21 0.2226354787941247 2307 22-23 0.2145324485792628 2307 24-25 0.28522967231238283 2307 26-27 -0.04991831922569645 2307 28-29 0.041901287629322326 2307 30-31 0.0416318644573721 2307 32-33 0.07837610952942953 2307 34-35 -0.2000021548214974 2307 36-37 -0.19159507969118295 2307 38-39 -0.22682310853774368 2307 40-41 0.055212797423422444 2307 42-43 0.026762128593858847 2307 44-45 0.14088164180690654 2307 46-47 -0.2050574717953566 2307 48-49 -0.0854264378288363 2307 50-51 -0.11891696194975054 2307 52-53 -0.08988032258964296 2307 54-55 -0.10939155734494932 2307 56-57 -0.30057044461400295 2307 58-59 0.19617798330792624 2307 60-61 0.04836065066620421 2307 62-63 -0.13246639447324782 2307 64-65 -0.10023354231859827 2307 66-67 -0.15185298172850992 2307 68-69 0.06845459592434366 2307 70-71 -0.3856451718203502 2307 72-73 -0.08617525604145015 2307 74-75 -0.11928094999277761 2307 76-77 -0.2057906574238899 2307 78-79 -0.8373761290753947 2307 80-81 -0.7383266518538818 2307 82-83 -0.6972302183445471 2307 84-85 -0.6102101051718023 2307 86-87 -0.7552531197414254 2307 88-89 -0.6786367854484574 2307 90-91 -1.0408935610127834 2307 92-93 -0.6998167626627882 2307 94-95 -1.0108941889291785 2307 96-97 -1.0809775860884834 2307 98-99 -1.1726325920627296 2307 100-101 -1.37078425510785 2201 1 0.28656598318442406 2201 2 0.41779615746347076 2201 3 0.33710808822027616 2201 4 0.42158331135310334 2201 5 0.40633826213915114 2201 6 0.25854708876138943 2201 7 0.43139562379695207 2201 8 0.43476902242965565 2201 9 0.17521873502274588 2201 10-11 0.34086360470816857 2201 12-13 0.22062675000233867 2201 14-15 0.5446179689090016 2201 16-17 0.4122673090769595 2201 18-19 0.3183086423536068 2201 20-21 0.39456550704598925 2201 22-23 0.4343697100831889 2201 24-25 0.3334707033120381 2201 26-27 0.347151033303426 2201 28-29 0.2197719482586109 2201 30-31 -0.03384512768629122 2201 32-33 0.006193556025039015 2201 34-35 0.06811967651871953 2201 36-37 -0.025997533346512114 2201 38-39 0.11053031071222819 2201 40-41 0.08784273666255871 2201 42-43 0.10444992610923975 2201 44-45 0.42980052411659386 2201 46-47 0.21935427014511788 2201 48-49 -0.07720243535196403 2201 50-51 0.10215389992570323 2201 52-53 -0.3339058266726269 2201 54-55 0.1443044061211296 2201 56-57 -0.21573260259226146 2201 58-59 0.015753044262304172 2201 60-61 0.03539091499531821 2201 62-63 -0.11716974986625672 2201 64-65 -0.03529778629089009 2201 66-67 -0.17323538816838635 2201 68-69 0.043864828518479726 2201 70-71 0.07902548106869034 2201 72-73 0.07553282089651248 2201 74-75 -0.23081777417328198 2201 76-77 -0.2695024883770927 2201 78-79 -0.6248340415333118 2201 80-81 -0.28190418968429043 2201 82-83 -0.4645441623826301 2201 84-85 -0.7824255546860996 2201 86-87 -0.6562554805139023 2201 88-89 -1.0069679902164417 2201 90-91 -0.5676844584933356 2201 92-93 -0.7106869588778117 2201 94-95 -0.41050814457761575 2201 96-97 -0.32905220197919505 2201 98-99 -0.7621532778477835 2201 100-101 -0.8725645097616699 1101 1 0.4049335670692429 1101 2 0.20986434895694828 1101 3 0.27236100048468614 1101 4 0.5399508952379222 1101 5 0.597241547870027 1101 6 0.37075150843707405 1101 7 0.4891958482638472 1101 8 0.3873890928658241 1101 9 0.4552024805237309 1101 10-11 0.42637387987361564 1101 12-13 0.5186017103336198 1101 14-15 0.37365547036029767 1101 16-17 0.6213069390935999 1101 18-19 0.4889228302091695 1101 20-21 0.4880675775595549 1101 22-23 0.49539664611012313 1101 24-25 0.2126988081148511 1101 26-27 0.40378538447800594 1101 28-29 0.415358077752785 1101 30-31 0.18669359285243559 1101 32-33 0.2512156157022716 1101 34-35 0.44042994394393276 1101 36-37 0.38710862401251234 1101 38-39 0.16978182973386424 1101 40-41 0.14317576038638435 1101 42-43 0.014416449581517554 1101 44-45 0.3276100368686343 1101 46-47 0.32272030598242196 1101 48-49 0.40840072266499305 1101 50-51 0.40268797632185027 1101 52-53 0.027403628027634852 1101 54-55 0.43999115870443006 1101 56-57 0.5096147405711804 1101 58-59 0.3119187240486738 1101 60-61 0.04527423091311533 1101 62-63 -0.012096024102874026 1101 64-65 0.06797633422461757 1101 66-67 0.027159363950495674 1101 68-69 0.15487434901076114 1101 70-71 0.30416964299445937 1101 72-73 0.33820746000792923 1101 74-75 0.34985485247636205 1101 76-77 0.5256908240575981 1101 78-79 0.5854633770974473 1101 80-81 0.36043878024488407 1101 82-83 0.05894262116162707 1101 84-85 0.30491335161832467 1101 86-87 -0.11482102097599522 1101 88-89 0.47722741208241004 1101 90-91 0.7353410068884507 1101 92-93 0.5239486694359776 1101 94-95 0.2360193913177362 1101 96-97 0.05482488304731703 1101 98-99 -0.1109041970009983 1101 100-101 0.007302164645235365 2304 1 -0.20463891342311769 2304 2 -0.003959514471269188 2304 3 -0.030016550161910516 2304 4 0.10545259872182555 2304 5 -0.008292027248124612 2304 6 -0.014226694295828679 2304 7 -0.0943664479591817 2304 8 -0.082928158114143 2304 9 -0.022487375504404383 2304 10-11 -0.09373822035717438 2304 12-13 -0.3162931499074304 2304 14-15 -0.09013175900556547 2304 16-17 -0.17341867177939463 2304 18-19 -9.661685819040144E-4 2304 20-21 -0.3192006204109177 2304 22-23 0.04766887382745466 2304 24-25 -0.11717718558409018 2304 26-27 0.11468310217591693 2304 28-29 0.10146827301233685 2304 30-31 0.0897049127776981 2304 32-33 -0.022014959086170904 2304 34-35 0.23596150418864426 2304 36-37 0.26939422316260675 2304 38-39 0.25744714094022214 2304 40-41 0.4852921101684089 2304 42-43 0.3498058696643085 2304 44-45 0.011714517214464593 2304 46-47 0.32073748735766117 2304 48-49 0.288991996431335 2304 50-51 0.7749816349656626 2304 52-53 0.4792435738092209 2304 54-55 0.2807703652106426 2304 56-57 0.4339562433004147 2304 58-59 0.24794191341013772 2304 60-61 0.11677934136481127 2304 62-63 0.13819521669281443 2304 64-65 0.508794006496295 2304 66-67 0.65744840670245 2304 68-69 0.3883203320858186 2304 70-71 0.48439091273106527 2304 72-73 0.5021906816073738 2304 74-75 0.4765903911802454 2304 76-77 0.3159974511594754 2304 78-79 0.18255738010143574 2304 80-81 0.3300508255245873 2304 82-83 0.6360344261218742 2304 84-85 0.37950946108090733 2304 86-87 0.42187257172649595 2304 88-89 0.2347694496689563 2304 90-91 0.35263869527911496 2304 92-93 0.3094287588230884 2304 94-95 0.06014383722753891 2304 96-97 0.30256272969713294 2304 98-99 0.37113137467354207 2304 100-101 0.7104517781558748 2202 1 0.10806020645650705 2202 2 0.021435362841465633 2202 3 0.07860728454021881 2202 4 0.18925317201895808 2202 5 0.06752273557330568 2202 6 -0.039445043166466576 2202 7 0.0788331720843587 2202 8 -0.041116135686621647 2202 9 -0.12432747436033509 2202 10-11 0.035753798035408124 2202 12-13 0.1582993636054013 2202 14-15 0.20137554170643313 2202 16-17 0.2223412831638285 2202 18-19 0.18402302955866645 2202 20-21 0.12743735337880935 2202 22-23 0.18778202015272427 2202 24-25 0.0046190333448024035 2202 26-27 0.3261221452236427 2202 28-29 0.30451801200241846 2202 30-31 0.4324180708715275 2202 32-33 0.48050687580089146 2202 34-35 0.8009071586468295 2202 36-37 0.6305335881645817 2202 38-39 0.4794904996880476 2202 40-41 0.47200624337173025 2202 42-43 0.24393503804721917 2202 44-45 0.5976586501655916 2202 46-47 0.46449888815288176 2202 48-49 0.36863574522296005 2202 50-51 0.07307933084918972 2202 52-53 0.3214397557512072 2202 54-55 0.2286020075135795 2202 56-57 0.24683189162213637 2202 58-59 0.338450567506861 2202 60-61 0.4390025910012483 2202 62-63 0.4366501725186751 2202 64-65 0.39879156872256516 2202 66-67 0.19534738199687496 2202 68-69 0.4047038527354445 2202 70-71 0.1726164656433653 2202 72-73 0.04982393956586151 2202 74-75 0.06560695963545271 2202 76-77 -0.014913519662897556 2202 78-79 0.1746766460793694 2202 80-81 0.37024887362438363 2202 82-83 0.20187533196497043 2202 84-85 -0.010399836924328554 2202 86-87 0.5060346080241089 2202 88-89 0.6853246736499266 2202 90-91 0.21340819116432286 2202 92-93 0.07047229409935696 2202 94-95 -0.10759180722736161 2202 96-97 -0.03568678064659636 2202 98-99 -0.019286493367243196 2202 100-101 0.1192832439303757 1102 1 0.11734879920278729 1102 2 0.16353327610005408 1102 3 0.22318694379888626 1102 4 0.2354647189207597 1102 5 -0.045664033767955914 1102 6 -0.05088820639512903 1102 7 0.14023045094163678 1102 8 0.2518735303549562 1102 9 0.19985558433176465 1102 10-11 0.16643647768269432 1102 12-13 0.1533478414412528 1102 14-15 0.3239945418261314 1102 16-17 0.289021249648286 1102 18-19 0.2008685787746387 1102 20-21 -0.004246802548252049 1102 22-23 0.2551123477272341 1102 24-25 0.2032265424219304 1102 26-27 0.18607107392331557 1102 28-29 0.3078376465234314 1102 30-31 0.12326114632139706 1102 32-33 -0.20510937125651907 1102 34-35 0.1741066958131725 1102 36-37 -0.13277487450947945 1102 38-39 -0.19363322851687315 1102 40-41 0.05511281481170016 1102 42-43 0.04774543584477442 1102 44-45 0.24588511946296165 1102 46-47 0.0386001529655573 1102 48-49 -0.23406145782536214 1102 50-51 0.07112824757902558 1102 52-53 0.1873975421295313 1102 54-55 -0.1458982518328682 1102 56-57 8.597414405926429E-4 1102 58-59 0.04813416484157784 1102 60-61 -0.2889589459256925 1102 62-63 -0.16754724997663573 1102 64-65 -0.29806435435128975 1102 66-67 0.19966849279766308 1102 68-69 0.04009865782661848 1102 70-71 0.26695872315443836 1102 72-73 -0.04399563510595783 1102 74-75 0.020712094695106487 1102 76-77 -0.07900401162316228 1102 78-79 -0.013054272007060774 1102 80-81 0.20389209271228026 1102 82-83 0.13908955213015162 1102 84-85 0.48699472877012084 1102 86-87 0.16954256796505973 1102 88-89 0.04072506466456716 1102 90-91 -6.48386260568401E-4 1102 92-93 0.2528566854331977 1102 94-95 0.2140536462299245 1102 96-97 -0.09466216268386063 1102 98-99 0.11061814693918492 1102 100-101 -0.4431195011520188 2305 1 -0.07460992991611448 2305 2 -0.0701385221311952 2305 3 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1213 54-55 -1.078364280883882 1213 56-57 -1.0697805385957935 1213 58-59 -0.9715758762267512 1213 60-61 -1.0525459473865126 1213 62-63 -1.0275704025909533 1213 64-65 -1.3445046399001939 1213 66-67 -1.3287513412752716 1213 68-69 -0.9423659930926007 1213 70-71 -1.0654880120513113 1213 72-73 -1.8927787447225626 1213 74-75 -0.8523509410536363 1213 76-77 -0.5617194825307976 1213 78-79 -0.7028450715991355 1213 80-81 -0.5778449046141461 1213 82-83 -0.7449489253098278 1213 84-85 -0.571201021071392 1213 86-87 -0.5456664946296854 1213 88-89 -0.7536108136188311 1213 90-91 -1.0932781373699605 1213 92-93 -0.5342825885669704 1213 94-95 -0.33352773245313116 1213 96-97 -0.5277896950010508 1213 98-99 0.11706504752075375 1213 100-101 -0.6078889397011231 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 6.0 5 22.0 6 47.0 7 280.0 8 315.0 9 337.0 10 427.0 11 579.0 12 724.0 13 854.0 14 909.0 15 1012.0 16 1126.0 17 1273.0 18 1339.0 19 1590.0 20 1716.0 21 1786.0 22 2109.0 23 2335.0 24 2603.0 25 3120.0 26 3705.0 27 4244.0 28 5066.0 29 6231.0 30 7618.0 31 9593.0 32 12839.0 33 16910.0 34 23419.0 35 35167.0 36 61195.0 37 104864.0 38 46285.0 39 74.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.66047959482697 19.970032234699573 9.238033628034788 25.131454542438668 2 17.59006101286329 20.06623779811847 36.57813765629881 25.765563532719426 3 20.653697401631618 25.273479723659005 29.437393393360185 24.635429481349195 4 12.945950951961011 16.553920498055163 38.577159017712255 31.922969532271573 5 13.546539273905495 35.52897853564675 35.039091441904695 15.885390748543054 6 30.836796138246786 37.43865789699227 18.135421245725063 13.589124719035889 7 25.470748607800743 30.34883785233563 23.927854492365714 20.25255904749791 8 25.313921495430474 34.72202635253778 21.13539895198197 18.828653200049775 9 23.69025728940565 15.198186116602841 21.090818597834954 40.020737996156555 10-11 27.245391427931526 23.13493500855769 28.1977973970243 21.42187616648648 12-13 25.03601677925655 21.829901254572956 28.343753542917344 24.790328423253154 14-15 24.085633506840036 23.03643640737438 22.950443444419097 29.927486641366485 16-17 24.335596856872687 27.540795956624404 24.380941058068224 23.742666128434685 18-19 23.791393978605218 27.365577573359655 25.821243816996937 23.02178463103819 20-21 24.332077161918928 25.788194569247487 25.5484805671296 24.331247701703987 22-23 24.206918672008715 25.4980520324394 26.046358586403212 24.248670709148673 24-25 24.154523243610964 26.349534242862934 25.501119282774106 23.994823230752 26-27 23.599546478990085 27.030266996667727 25.33440261051118 24.035783913831008 28-29 23.70453015239683 26.1502345470608 26.10875849740567 24.036476803136694 30-31 23.725275226630796 26.542727698797066 26.052345250258185 23.679651824313954 32-33 23.889415066816916 26.6720129416726 26.014835913141464 23.423736078369018 34-35 24.037824706075927 26.03552668511167 26.21541388574328 23.711234723069115 36-37 24.430884022439685 25.868832646785116 26.236236194711438 23.464047136063762 38-39 23.917005196162748 26.067508634921865 25.921772723878846 24.093713445036542 40-41 24.14537419349486 25.763224195292473 25.624669861805188 24.46673174940748 42-43 24.296811696805612 25.60307851428224 25.710931601784274 24.38917818712787 44-45 23.890860017311518 25.68063847968386 26.029219405490462 24.399282097514167 46-47 24.101141694885257 25.45784633508614 26.32452231192869 24.116489658099923 48-49 23.758685296700104 25.7032238438617 26.076285423911617 24.46180543552658 50-51 23.417227041236103 25.87831194852712 26.25360043253588 24.450860577700894 52-53 23.028980516939296 26.225729145491684 26.378396467632175 24.366893869936845 54-55 22.87484184982128 25.96331607220636 26.784245131048596 24.377596946923763 56-57 22.660470415481594 26.213543906320645 26.900321196626813 24.225664481570945 58-59 22.669985136022678 26.158111237858208 27.064053372048807 24.10785025407031 60-61 23.359639576352865 25.93559556882468 26.648311758710864 24.05645309611159 62-63 23.2890901145585 24.88674141640991 27.234194965178865 24.58997350385273 64-65 23.36510245680607 25.264335517345334 26.558009995381216 24.812552030467383 66-67 23.290901496731863 25.396449998755262 26.198754705812387 25.11389379870049 68-69 23.480235489683842 25.230780719939037 25.858642573735736 25.430341216641384 70-71 23.50111745441671 25.363172685431053 26.126305540803685 25.009404319348555 72-73 23.38910664051803 25.335317531664654 26.13996896463019 25.135606863187128 74-75 23.530241589723445 25.422380933940396 25.83176127593895 25.21561620039721 76-77 23.372775335515232 25.49639101530187 26.00557739605322 25.12525625312968 78-79 23.358605066701333 25.95543471345948 25.90218981736309 24.783770402476097 80-81 23.516557381357092 25.571517680431405 26.218654525186334 24.693270413025168 82-83 23.62674897460789 25.477847934972303 26.363432705790597 24.53197038462921 84-85 23.77840433755132 25.552115468912696 26.25678779489832 24.41269239863767 86-87 23.9556740580115 25.47441702231233 26.27790423981258 24.292004679863588 88-89 24.450550970283008 24.685377160179677 26.48999845108756 24.37407341844976 90-91 24.594856590503348 24.841420529109897 26.155828962343342 24.40789391804342 92-93 24.324578522890263 24.71586861836837 26.390297001492407 24.569255857248965 94-95 24.680230668254623 24.264291928962585 26.15849382316448 24.896983579618308 96-97 25.26301957258455 24.501279479908707 25.908707379486824 24.32699356801992 98-99 25.11819954905731 24.573610169725978 26.065455853263803 24.242734427952914 100-101 25.369729683369464 24.415508242575317 25.92804190938355 24.286720164671674 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2.0 1 2.0 2 4.5 3 4.5 4 7.0 5 14.5 6 17.0 7 18.0 8 19.5 9 18.5 10 17.0 11 24.0 12 26.0 13 30.5 14 39.5 15 47.0 16 75.0 17 103.5 18 135.0 19 210.5 20 302.5 21 442.0 22 640.0 23 885.5 24 1227.5 25 1586.5 26 2059.5 27 2543.5 28 3032.5 29 3669.0 30 4231.0 31 4841.0 32 5601.0 33 6017.5 34 6201.0 35 6517.0 36 7270.5 37 7893.5 38 8030.5 39 8369.0 40 8881.0 41 9148.5 42 9352.0 43 9798.0 44 10405.5 45 10905.5 46 11127.5 47 11270.5 48 11418.0 49 11814.5 50 12336.0 51 12738.0 52 12756.5 53 12404.5 54 11980.0 55 11277.5 56 10475.5 57 9677.0 58 8694.5 59 7721.5 60 7013.5 61 6529.0 62 5865.5 63 5265.0 64 4997.5 65 4757.5 66 4484.5 67 4193.0 68 3827.5 69 3455.5 70 3036.0 71 2576.0 72 2137.5 73 1740.0 74 1413.0 75 1084.0 76 766.0 77 570.5 78 443.0 79 317.0 80 222.0 81 156.0 82 116.0 83 85.5 84 67.0 85 55.5 86 40.5 87 35.5 88 33.0 89 20.5 90 14.5 91 11.5 92 8.0 93 8.5 94 7.5 95 5.5 96 2.5 97 2.5 98 3.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0013822578906191685 2 0.0 3 0.0 4 0.0 5 0.003040967359362171 6 0.007187741031219676 7 0.019075158890544525 8 0.0254335451873927 9 0.019904513624916027 10-11 0.01769290099992536 12-13 0.024880642031145036 14-15 0.019213384679606444 16-17 0.013546127328067852 18-19 0.013822578906191686 20-21 0.01271677259369635 22-23 0.018798707312420695 24-25 0.030824350960807458 26-27 0.030962576749869377 28-29 0.020042739413977946 30-31 0.019489836257730278 32-33 0.028889189913940627 34-35 0.030824350960807458 36-37 0.03745918883577947 38-39 0.03151547990611704 40-41 0.03759741462484139 42-43 0.03427999568735538 44-45 0.03262128621861238 46-47 0.0319301572733028 48-49 0.0344182214764173 50-51 0.03828854357015097 52-53 0.04326467197637998 54-55 0.0344182214764173 56-57 0.030547899382683624 58-59 0.03165370569517896 60-61 0.04215886566388464 62-63 0.045614510390432565 64-65 0.04423225249981339 66-67 0.05694902509350974 68-69 0.05045241300759965 70-71 0.05280225142165224 72-73 0.056396121937262074 74-75 0.05667257351538591 76-77 0.07408902293718744 78-79 0.05252579984352841 80-81 0.058469508773190834 82-83 0.04367934934356573 84-85 0.03884144672639864 86-87 0.049208380906042404 88-89 0.05059063879666157 90-91 0.04354112355450381 92-93 0.06372208875754368 94-95 0.07063337821063952 96-97 0.0696657976872061 98-99 0.07243031346844443 100-101 0.07754466766373536 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 361727.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.50822658876702 #Duplication Level Percentage of deduplicated Percentage of total 1 96.65702597550208 90.38227086313587 2 2.3694982469328836 4.4313515795177265 3 0.4479984511687375 1.2567462204990896 4 0.2059142159027968 0.7701869263394806 5 0.08605990668766526 0.40236546273791746 6 0.06783466023245872 0.38058592677532643 7 0.03648855937451153 0.23883863345226525 8 0.019726222243817773 0.1475651247452231 9 0.017726867719113727 0.14918471670491876 >10 0.08545531116217879 1.3869295960271932 >50 0.005325462566843502 0.3424576342704355 >100 9.461205069129868E-4 0.11151731579452029 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-11 0.0 0.0 0.0 12-13 0.0 0.0 0.0 14-15 0.0 0.0 0.0 16-17 1.3822578906191687E-4 0.0 0.0 18-19 2.7645157812383373E-4 2.7645157812383373E-4 0.0 20-21 2.7645157812383373E-4 2.7645157812383373E-4 1.3822578906191687E-4 22-23 2.7645157812383373E-4 2.7645157812383373E-4 9.675805234334179E-4 24-25 2.7645157812383373E-4 2.7645157812383373E-4 0.00221161262499067 26-27 2.7645157812383373E-4 5.529031562476675E-4 0.006911289453095843 28-29 2.7645157812383373E-4 5.529031562476675E-4 0.03345064095298388 30-31 2.7645157812383373E-4 5.529031562476675E-4 0.05957531508568617 32-33 2.7645157812383373E-4 5.529031562476675E-4 0.09150547235898895 34-35 2.7645157812383373E-4 5.529031562476675E-4 0.1687736884446005 36-37 2.7645157812383373E-4 5.529031562476675E-4 0.35219931052976416 38-39 2.7645157812383373E-4 5.529031562476675E-4 0.6920965258330177 40-41 2.7645157812383373E-4 5.529031562476675E-4 1.0834137346673043 42-43 2.7645157812383373E-4 5.529031562476675E-4 1.5651306095480846 44-45 2.7645157812383373E-4 5.529031562476675E-4 2.216588753396899 46-47 2.7645157812383373E-4 5.529031562476675E-4 2.9486325322688103 48-49 2.7645157812383373E-4 5.529031562476675E-4 3.6089371266175876 50-51 2.7645157812383373E-4 5.529031562476675E-4 4.2948134919428185 52-53 2.7645157812383373E-4 5.529031562476675E-4 4.968525987830601 54-55 2.7645157812383373E-4 5.529031562476675E-4 5.705269443530618 56-57 2.7645157812383373E-4 5.529031562476675E-4 6.524395469511537 58-59 5.529031562476675E-4 5.529031562476675E-4 7.293621985641105 60-61 5.529031562476675E-4 5.529031562476675E-4 8.04322043972388 62-63 8.293547343715011E-4 5.529031562476675E-4 8.834701307892416 64-65 8.293547343715011E-4 5.529031562476675E-4 9.665714751732661 66-67 8.293547343715011E-4 5.529031562476675E-4 10.421948043690406 68-69 8.293547343715011E-4 5.529031562476675E-4 11.16270004727322 70-71 8.293547343715011E-4 5.529031562476675E-4 11.94948123861365 72-73 8.293547343715011E-4 5.529031562476675E-4 12.644895183384154 74-75 8.293547343715011E-4 5.529031562476675E-4 13.32607187188128 76-77 8.293547343715011E-4 5.529031562476675E-4 14.014989204565873 78-79 8.293547343715011E-4 5.529031562476675E-4 14.681099282055252 80-81 8.293547343715011E-4 5.529031562476675E-4 15.343339037450896 82-83 8.293547343715011E-4 5.529031562476675E-4 16.000049761284064 84-85 8.293547343715011E-4 5.529031562476675E-4 16.65164613092194 86-87 8.293547343715011E-4 5.529031562476675E-4 17.319691369458184 88-89 8.293547343715011E-4 5.529031562476675E-4 18.059614018306625 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACATAC 45 9.635827E-4 42.204834 1 TCTATAC 55 0.0025825095 34.531227 3 TAATACT 115 1.0368058E-7 33.029873 4 GTATACT 75 3.0817447E-4 31.653625 4 TGTACCG 140 1.7058483E-8 30.523138 5 TATACTC 95 3.4941106E-5 29.987646 5 CCTTATG 65 0.005850611 29.218733 2 CCGCGTA 85 6.4088707E-4 27.933533 9 ATAGGGC 85 6.414055E-4 27.92967 3 TATTAGG 105 6.91233E-5 27.13168 2 TACACCC 70 0.008400965 27.13168 5 CAGACTG 125 7.396222E-6 26.589045 5 TACACAC 90 8.9571625E-4 26.378021 3 CCCTGTA 95 0.0012268847 24.99316 7 GTGTTAG 95 0.0012278742 24.989706 1 TGTATAC 95 0.0012278742 24.989706 3 AAGACCC 100 0.0016554268 23.740221 5 TTGTACC 100 0.0016554268 23.740221 4 TATAGGG 100 0.0016554268 23.740221 2 GTATAGG 160 1.860346E-6 23.74022 1 >>END_MODULE