FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L93_CAGAGAGG-ACTGCATA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L93_CAGAGAGG-ACTGCATA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361727
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAC459.635827E-442.2048341
TCTATAC550.002582509534.5312273
TAATACT1151.0368058E-733.0298734
GTATACT753.0817447E-431.6536254
TGTACCG1401.7058483E-830.5231385
TATACTC953.4941106E-529.9876465
CCTTATG650.00585061129.2187332
CCGCGTA856.4088707E-427.9335339
ATAGGGC856.414055E-427.929673
TATTAGG1056.91233E-527.131682
TACACCC700.00840096527.131685
CAGACTG1257.396222E-626.5890455
TACACAC908.9571625E-426.3780213
CCCTGTA950.001226884724.993167
GTGTTAG950.001227874224.9897061
TGTATAC950.001227874224.9897063
AAGACCC1000.001655426823.7402215
TTGTACC1000.001655426823.7402214
TATAGGG1000.001655426823.7402212
GTATAGG1601.860346E-623.740221
Produced by FastQC (version 0.11.2)