##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename G140_A6_L93_CAGAGAGG-ACTGCATA_L006_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 361727 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74712144794279 33.0 33.0 33.0 33.0 33.0 2 32.784246130369034 33.0 33.0 33.0 33.0 33.0 3 32.79204206487213 33.0 33.0 33.0 33.0 33.0 4 36.75380328258604 37.0 37.0 37.0 37.0 37.0 5 36.65570996912036 37.0 37.0 37.0 37.0 37.0 6 36.660716507200185 37.0 37.0 37.0 37.0 37.0 7 36.615494558050685 37.0 37.0 37.0 37.0 37.0 8 36.61961645108051 37.0 37.0 37.0 37.0 37.0 9 36.66470846798829 37.0 37.0 37.0 37.0 37.0 10-11 36.63371686382271 37.0 37.0 37.0 37.0 37.0 12-13 36.61224763426562 37.0 37.0 37.0 37.0 37.0 14-15 38.89586621955232 40.0 40.0 40.0 37.0 40.0 16-17 38.83955579760428 40.0 38.5 40.0 37.0 40.0 18-19 38.77171319807479 40.0 37.0 40.0 37.0 40.0 20-21 38.72730263430709 40.0 37.0 40.0 37.0 40.0 22-23 38.67151747035748 40.0 37.0 40.0 37.0 40.0 24-25 38.606109026973385 40.0 37.0 40.0 37.0 40.0 26-27 38.507815286113555 40.0 37.0 40.0 37.0 40.0 28-29 38.42837692513967 40.0 37.0 40.0 37.0 40.0 30-31 38.31692132464538 40.0 37.0 40.0 37.0 40.0 32-33 38.173253586268096 40.0 37.0 40.0 37.0 40.0 34-35 38.04616050225722 40.0 37.0 40.0 35.0 40.0 36-37 37.90779925192203 40.0 37.0 40.0 33.0 40.0 38-39 37.72435704274218 40.0 37.0 40.0 33.0 40.0 40-41 37.53725737918375 40.0 37.0 40.0 33.0 40.0 42-43 37.32999886654853 40.0 37.0 40.0 33.0 40.0 44-45 37.09017297575243 37.0 37.0 40.0 33.0 40.0 46-47 36.88870750593679 37.0 37.0 40.0 33.0 40.0 48-49 36.828493587705644 37.0 37.0 40.0 33.0 40.0 50-51 37.058534474894046 37.0 37.0 40.0 33.0 40.0 52-53 36.973099879190656 37.0 37.0 40.0 33.0 40.0 54-55 36.764280797396935 37.0 37.0 40.0 33.0 40.0 56-57 36.558230378158115 37.0 37.0 40.0 33.0 40.0 58-59 36.35256146209711 37.0 37.0 40.0 33.0 40.0 60-61 36.16614325167875 37.0 37.0 40.0 33.0 40.0 62-63 35.94594127615578 37.0 37.0 37.0 33.0 40.0 64-65 35.74997166371324 37.0 35.0 37.0 33.0 40.0 66-67 35.5363658228443 37.0 33.0 37.0 33.0 40.0 68-69 35.35003054789938 37.0 33.0 37.0 33.0 40.0 70-71 35.173028278231925 37.0 33.0 37.0 33.0 40.0 72-73 34.972106035767304 37.0 33.0 37.0 33.0 37.0 74-75 34.77824574886586 37.0 33.0 37.0 30.0 37.0 76-77 33.55686056058851 35.0 33.0 37.0 27.0 37.0 78-79 34.64553931556118 37.0 33.0 37.0 33.0 37.0 80-81 34.710501289646615 37.0 33.0 37.0 33.0 37.0 82-83 34.60122412758793 37.0 33.0 37.0 33.0 37.0 84-85 34.50727205876255 37.0 33.0 37.0 30.0 37.0 86-87 34.417494408766835 37.0 33.0 37.0 27.0 37.0 88-89 34.31045373997517 37.0 33.0 37.0 27.0 37.0 90-91 34.20310897444758 37.0 33.0 37.0 27.0 37.0 92-93 34.129062801505 37.0 33.0 37.0 27.0 37.0 94-95 34.0896767451697 37.0 33.0 37.0 27.0 37.0 96-97 33.96914800388138 37.0 33.0 37.0 27.0 37.0 98-99 33.869698695425 37.0 33.0 37.0 27.0 37.0 100-101 33.01683037207618 35.0 33.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.03915612822736847 2306 2 -0.0847012459794314 2306 3 0.036812252930090494 2306 4 -0.14637333307353373 2306 5 0.022522794241034205 2306 6 -0.22486404776590518 2306 7 -0.12075816274177242 2306 8 -0.047058664118317495 2306 9 -0.11817001227600343 2306 10-11 0.0017761944945746677 2306 12-13 0.043804135850038506 2306 14-15 -0.07229280599680266 2306 16-17 0.009528898131691221 2306 18-19 -0.1359712201541825 2306 20-21 -0.0024531344328053706 2306 22-23 -0.1327812940246602 2306 24-25 0.019548998192256306 2306 26-27 -0.07594246365483315 2306 28-29 -0.14063068818482094 2306 30-31 -0.05498854079758786 2306 32-33 -0.21195332401594413 2306 34-35 -0.08161285878349389 2306 36-37 -0.25790634095194065 2306 38-39 -0.20550765264034965 2306 40-41 -0.1504210232046077 2306 42-43 -0.18932384218653198 2306 44-45 -0.17013930084718254 2306 46-47 -0.6773419373977418 2306 48-49 -0.6837253467512241 2306 50-51 -0.4550517859206096 2306 52-53 -0.23494912594547657 2306 54-55 -0.34559858297809853 2306 56-57 -0.1397538895528001 2306 58-59 -0.24019823324908884 2306 60-61 -0.3041207021988228 2306 62-63 -0.1308978274066277 2306 64-65 -0.38976518080902167 2306 66-67 -0.32759703595969114 2306 68-69 -0.4169314179015018 2306 70-71 -0.428462788761351 2306 72-73 -0.5191634284777464 2306 74-75 -0.6970321010689489 2306 76-77 -0.7800567960943638 2306 78-79 -0.5124300934697388 2306 80-81 -0.5116712969603725 2306 82-83 -0.1857677909619042 2306 84-85 -0.03448645154601593 2306 86-87 0.09669620750315744 2306 88-89 -0.26009273950391787 2306 90-91 -0.21308360992185982 2306 92-93 -0.5051565647561276 2306 94-95 -0.36976109295616055 2306 96-97 -0.25405254053897863 2306 98-99 0.06753191255120328 2306 100-101 -0.21593797218351796 2307 1 0.05283108942973058 2307 2 0.10643844676797443 2307 3 -0.008690109024570347 2307 4 0.08541579796533227 2307 5 -0.06616959589830174 2307 6 -0.05027788604147787 2307 7 0.00816685015966101 2307 8 -0.261123210980287 2307 9 0.0238648414415934 2307 10-11 0.1158298247593521 2307 12-13 -0.12931324734236682 2307 14-15 0.02276594514447794 2307 16-17 -0.18541175527627018 2307 18-19 -0.24041328279560048 2307 20-21 0.029730978941017838 2307 22-23 -0.022255709490501374 2307 24-25 -0.06418626440152053 2307 26-27 -0.07168995283747392 2307 28-29 -0.0035182273853209267 2307 30-31 0.06354585640776378 2307 32-33 -0.12951812890658942 2307 34-35 -0.10822950758663552 2307 36-37 -0.3407434653217507 2307 38-39 -0.3786696946394841 2307 40-41 -0.1919437892718534 2307 42-43 -0.4171581877861499 2307 44-45 -0.15013215543810787 2307 46-47 -0.1871420644272348 2307 48-49 -0.3348457865908543 2307 50-51 -0.45442160053694636 2307 52-53 -0.32609278836459765 2307 54-55 -0.3334365012822502 2307 56-57 -0.2914945905968196 2307 58-59 -0.49329953927108505 2307 60-61 -0.4542437620217825 2307 62-63 -0.028832605700472413 2307 64-65 -0.3075086209265194 2307 66-67 -0.5448968652638086 2307 68-69 -0.4688150271270146 2307 70-71 -0.37512528727272354 2307 72-73 -0.5695683349919847 2307 74-75 -0.44794016347218957 2307 76-77 -0.6689804196107048 2307 78-79 -0.41323295957271 2307 80-81 -0.13097481805917965 2307 82-83 0.04032486117576184 2307 84-85 0.09070706994975097 2307 86-87 0.19404744405589724 2307 88-89 0.1606180302154243 2307 90-91 -0.026032679033839656 2307 92-93 -0.021055099947261624 2307 94-95 -0.03195195477189117 2307 96-97 -0.18418135638590627 2307 98-99 0.022401707319168906 2307 100-101 -0.15938999870092374 2201 1 0.012069063035525573 2201 2 0.07334893091984185 2201 3 0.1540193611561591 2201 4 0.07036103578413133 2201 5 0.1498701115207126 2201 6 0.19412011697721 2201 7 0.13681927478909728 2201 8 0.10489327572584983 2201 9 0.03137287193813165 2201 10-11 0.08813428700634063 2201 12-13 0.16986628746934684 2201 14-15 0.13747142910283827 2201 16-17 0.19176034890733007 2201 18-19 0.25389764254980207 2201 20-21 0.22516230368268708 2201 22-23 0.39566874579027456 2201 24-25 0.30167057990359325 2201 26-27 0.22042177749264624 2201 28-29 0.22141307800574594 2201 30-31 0.2970107737807268 2201 32-33 0.44989187148042475 2201 34-35 0.17303137712252692 2201 36-37 0.15547349353887796 2201 38-39 0.36168887186850895 2201 40-41 0.17544658961277548 2201 42-43 0.3871057156156823 2201 44-45 0.36153625278202384 2201 46-47 0.1513530398724683 2201 48-49 0.2366498208242973 2201 50-51 0.27599076062254824 2201 52-53 0.23569365847932744 2201 54-55 0.1828615365675077 2201 56-57 0.21138864791860357 2201 58-59 0.11896776983530799 2201 60-61 0.24962442173146115 2201 62-63 0.12701224123632926 2201 64-65 0.06326327427066758 2201 66-67 0.17777042062186865 2201 68-69 0.37123296234626224 2201 70-71 0.19698953915245454 2201 72-73 0.030716118740748755 2201 74-75 0.10551101497898685 2201 76-77 0.17711946815585122 2201 78-79 0.4291225882426062 2201 80-81 0.6618915854394132 2201 82-83 0.4639867675962819 2201 84-85 0.5800545679179407 2201 86-87 0.44160443155540463 2201 88-89 0.48309084263075874 2201 90-91 0.544171276227587 2201 92-93 0.2878964848663941 2201 94-95 0.5234391207341318 2201 96-97 0.5454666563080792 2201 98-99 0.3544917649839405 2201 100-101 0.6296304742275964 1101 1 -0.05828002168137658 1101 2 -0.0015862445900438615 1101 3 0.06538396504949873 1101 4 0.011341723891256095 1101 5 0.08197855224984352 1101 6 -0.028672947769877055 1101 7 0.08532734398681896 1101 8 0.007395307538232032 1101 9 0.11645743403418862 1101 10-11 -0.0029973357344772467 1101 12-13 0.02037811068232287 1101 14-15 -0.0914315857197181 1101 16-17 -0.18078212564663687 1101 18-19 -0.153993529709183 1101 20-21 -0.05205914451576632 1101 22-23 -0.20435447492260295 1101 24-25 -0.017889968105230025 1101 26-27 0.06102609654524116 1101 28-29 -0.03901205454581458 1101 30-31 0.0018174613460359978 1101 32-33 0.21153125380946136 1101 34-35 -0.04804432240144507 1101 36-37 -0.15092865050694115 1101 38-39 -0.0962622872320722 1101 40-41 -0.3616968756916066 1101 42-43 -0.16715818778614988 1101 44-45 0.01807772110510797 1101 46-47 -0.24269761998279193 1101 48-49 0.05712952205112032 1101 50-51 0.09650432538898457 1101 52-53 0.05816647089466187 1101 54-55 0.24835362217454104 1101 56-57 0.21467824890935816 1101 58-59 0.26595971998817447 1101 60-61 0.15223771945970554 1101 62-63 0.16561183874397045 1101 64-65 0.04897286055496153 1101 66-67 -0.02174871711565629 1101 68-69 0.20865410867545364 1101 70-71 -0.2022857810998815 1101 72-73 -0.13438314980679422 1101 74-75 0.388479589614235 1101 76-77 0.087192419895473 1101 78-79 0.03738432437790351 1101 80-81 0.43538320663218144 1101 82-83 0.2563742438918126 1101 84-85 0.3345342304435803 1101 86-87 0.03664003664849247 1101 88-89 0.39981556107962035 1101 90-91 0.1807574444229445 1101 92-93 0.25363625807742807 1101 94-95 0.3538505143639128 1101 96-97 0.2139667917622461 1101 98-99 0.2307350406525046 1101 100-101 0.21561000129907626 2304 1 0.06057003510142067 2304 2 -0.013904448055626517 2304 3 0.02053471421607611 2304 4 0.061933363998228685 2304 5 -0.020422810537276348 2304 6 0.057426257270968506 2304 7 0.026254918445552278 2304 8 -0.028405329894454212 2304 9 -0.12695759325819012 2304 10-11 0.016336290671354448 2304 12-13 0.04772085896967582 2304 14-15 0.17725636533717193 2304 16-17 0.20442046071816122 2304 18-19 0.2180986415916948 2304 20-21 0.17812640701003346 2304 22-23 0.09161211481253417 2304 24-25 0.03361294430272466 2304 26-27 0.10582497554293724 2304 28-29 0.16455885068186404 2304 30-31 0.06314288172189464 2304 32-33 -0.03860612392435314 2304 34-35 0.1758126948531995 2304 36-37 0.11545135462182543 2304 38-39 0.10247750102304565 2304 40-41 0.14065686281445977 2304 42-43 0.01946946360411772 2304 44-45 0.04785663453881028 2304 46-47 0.1777011417859029 2304 48-49 0.21857125557495038 2304 50-51 0.1915605586746949 2304 52-53 0.18326721456612205 2304 54-55 0.24010179962993305 2304 56-57 -0.042804258325865874 2304 58-59 0.15146453736737442 2304 60-61 0.060034363779593036 2304 62-63 -0.12306915184107226 2304 64-65 0.06068660199174758 2304 66-67 0.1477433516098401 2304 68-69 0.10199861539779675 2304 70-71 -0.21611056037966137 2304 72-73 -0.13092123091460905 2304 74-75 -0.0474428560757687 2304 76-77 -0.23264584071925043 2304 78-79 0.03439406903849118 2304 80-81 -0.2277207974708304 2304 82-83 -0.12936411563487837 2304 84-85 -0.2492478701535532 2304 86-87 -0.25172040589099254 2304 88-89 -0.38139666004357764 2304 90-91 -0.4810094164042482 2304 92-93 -0.3027893564589732 2304 94-95 -0.536228981551389 2304 96-97 -0.4668818362921243 2304 98-99 -0.5400181556694363 2304 100-101 -0.16358459884013854 2202 1 0.15429823173729318 2202 2 0.013583552213368932 2202 3 0.006479687778842447 2202 4 0.07593795373772139 2202 5 0.010553727992160589 2202 6 0.03701620691833796 2202 7 0.05453309364582992 2202 8 0.1887246538817422 2202 9 -0.0800766827076771 2202 10-11 0.09526972263645206 2202 12-13 0.028924782944763194 2202 14-15 0.0011566351735012859 2202 16-17 0.1325944000764352 2202 18-19 0.12056667733450155 2202 20-21 0.12240226212222183 2202 22-23 0.3084983212163124 2202 24-25 -0.03928069310784821 2202 26-27 -0.05516055753093241 2202 28-29 0.05861236401816683 2202 30-31 -0.06664335074083994 2202 32-33 -0.064148108272164 2202 34-35 -0.08241899452399792 2202 36-37 -0.06794068142360032 2202 38-39 0.2312129689495066 2202 40-41 -0.0895236165022979 2202 42-43 -0.06148984238692634 2202 44-45 0.0428171678228324 2202 46-47 -0.20607589438562002 2202 48-49 -0.05547233854412781 2202 50-51 -0.09439029916947561 2202 52-53 -0.05022107151082622 2202 54-55 -0.012628086635309899 2202 56-57 0.11718935302574351 2202 58-59 0.20636908591690428 2202 60-61 0.14208226183021821 2202 62-63 0.2818072362188815 2202 64-65 0.1182642955215556 2202 66-67 0.2520464425388056 2202 68-69 -0.025349843340777056 2202 70-71 -0.10664611404850177 2202 72-73 -0.21984112903248132 2202 74-75 -0.25529248746757105 2202 76-77 -0.04873682852197447 2202 78-79 0.03286835893181461 2202 80-81 0.07593334128052476 2202 82-83 0.09908050066793805 2202 84-85 0.01566247861621406 2202 86-87 0.031922973031512925 2202 88-89 0.08345316468889763 2202 90-91 0.14622976282256417 2202 92-93 -0.004687457517299265 2202 94-95 0.12389912766087008 2202 96-97 0.3404793997432165 2202 98-99 0.14638747306603506 2202 100-101 0.21781770947939805 1102 1 -0.016974161830916046 1102 2 -0.03597156888147168 1102 3 0.010445693444559367 1102 4 -0.05268192416994566 1102 5 0.026553408796530675 1102 6 0.05853797207015532 1102 7 0.03394497570304367 1102 8 0.136320711815749 1102 9 0.07442996055131346 1102 10-11 0.07857978128865284 1102 12-13 0.07035637533194006 1102 14-15 -0.06586646661000373 1102 16-17 0.07776856119043174 1102 18-19 -0.15632355926926067 1102 20-21 -0.3345621874648259 1102 22-23 -0.22386412541816725 1102 24-25 -0.12416279022312438 1102 26-27 -0.09010848874427069 1102 28-29 -0.02314959584298748 1102 30-31 -0.08644544945208565 1102 32-33 -0.008552209935970723 1102 34-35 -0.1428147971015008 1102 36-37 -0.303610792743072 1102 38-39 -0.34875750545499073 1102 40-41 -0.1610274269000911 1102 42-43 -0.3137022670767138 1102 44-45 -0.5649991350938208 1102 46-47 -0.6789043666355496 1102 48-49 -0.1075331453110735 1102 50-51 -0.16599784814604845 1102 52-53 -0.2906033082741786 1102 54-55 -0.34508230201256396 1102 56-57 -0.5120649261906252 1102 58-59 -0.19820738138550098 1102 60-61 -0.3708930404081414 1102 62-63 -0.6224380656204858 1102 64-65 -0.3514879288729631 1102 66-67 -0.31163308505167464 1102 68-69 -0.393454220310808 1102 70-71 -0.20562867798737727 1102 72-73 -0.3634911310274518 1102 74-75 -0.6567168979531459 1102 76-77 -0.3431110055957518 1102 78-79 -0.3794605721618254 1102 80-81 -0.42571051619862743 1102 82-83 -0.3005723740876718 1102 84-85 -0.07614217366005249 1102 86-87 -0.3310742739061965 1102 88-89 -0.4877952892072841 1102 90-91 -0.2839182641494702 1102 92-93 -0.28146198570625813 1102 94-95 -0.14477581149245822 1102 96-97 -0.05262597471315189 1102 98-99 -0.29592988718612645 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1213 76-77 -0.9845955291755857 1213 78-79 -0.6672585931645543 1213 80-81 -0.5397261166506553 1213 82-83 -0.4973282518198232 1213 84-85 -2.604027292592825 1213 86-87 -1.2122214308202928 1213 88-89 -2.139957578008591 1213 90-91 -2.5954559836169615 1213 92-93 -1.7466476200603296 1213 94-95 -1.4925170534648444 1213 96-97 -1.5903233920941489 1213 98-99 -0.9207169658223293 1213 100-101 -0.6916554670394461 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 10.0 15 27.0 16 66.0 17 106.0 18 166.0 19 255.0 20 353.0 21 424.0 22 611.0 23 758.0 24 1061.0 25 1306.0 26 1757.0 27 2226.0 28 2876.0 29 3630.0 30 4811.0 31 6418.0 32 8842.0 33 12412.0 34 18553.0 35 31700.0 36 68038.0 37 132282.0 38 62915.0 39 121.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.06684222027367 19.952534140289828 9.606027832254103 25.37459580718239 2 17.54406820896183 19.607311894545937 36.729221037731676 26.11939885876056 3 20.680293173567932 24.539047877090493 29.907353392480445 24.87330555686113 4 12.963367401382811 16.197021510697294 38.69907416366487 32.14053692425503 5 13.303402842475126 35.38331393564761 35.218548795085795 16.094734426791476 6 31.052423512759624 37.17637887135879 18.16120997326713 13.609987642614458 7 25.586699361673304 30.260113289856715 23.855006676305614 20.298180672164367 8 25.266844885784028 34.97333624528996 20.950882848114738 18.808936020811277 9 23.28081674854241 15.031778108905336 21.177020239020035 40.51038490353222 10-11 27.37188542740796 22.84526728720831 28.378583849145905 21.404263436237827 12-13 25.048862816433388 21.507103423299892 28.455990291020576 24.988043469246144 14-15 23.944024084461486 22.92530001907516 22.988054527309266 30.14262136915409 16-17 24.340593873280124 27.37230010477515 24.436246119310972 23.850859902633754 18-19 23.797642973844916 27.13579577969021 26.099793490671143 22.966767755793736 20-21 24.405698219928286 25.48620921302529 25.74911466382106 24.35897790322536 22-23 24.323315649647387 25.31356520248696 26.169735739936474 24.193383407929183 24-25 24.10450422556237 26.135732195827238 25.823203686758244 23.936559891852145 26-27 23.59818316022857 26.741714055074684 25.552281140196886 24.107821644499857 28-29 23.587678000259864 25.961014798452975 26.263729276498577 24.187577924788584 30-31 23.64269186430651 26.374724585115295 26.24976847180332 23.732815078774877 32-33 23.86938215836805 26.379700713521526 26.18715218935827 23.56376493875215 34-35 24.108374547656105 25.850572392992504 26.322198785271766 23.71885427407962 36-37 24.195318568976052 25.6741962861495 26.54847440196612 23.582010742908327 38-39 23.853762644204053 25.925628996453128 26.058740431319755 24.161867928023067 40-41 24.153574380679352 25.52601824027512 25.77731272478969 24.543094654255835 42-43 24.30064662024123 25.225100697487328 26.04201511084326 24.432237571428175 44-45 23.851827483157187 25.483030019876868 26.328695397357677 24.336447099608268 46-47 24.122750029718546 25.22855634221388 26.493045860552293 24.15564776751528 48-49 23.688029923118815 25.45317324943949 26.400019904513627 24.458776922928063 50-51 23.409090280791865 25.547581463368786 26.56423214191918 24.479096113920164 52-53 22.808222775739715 26.04505607820262 26.542668918825523 24.60405222723214 54-55 22.8086374531069 25.64240435466526 27.02272708423756 24.526231107990277 56-57 22.479383623561418 26.01257301777307 26.984853218034594 24.52319014063092 58-59 22.561489742264193 25.88830803340641 27.222186897853906 24.32801532647549 60-61 23.21018337033177 25.588772748509236 26.808062433824404 24.39298144733459 62-63 22.901386957567446 24.821481393426534 27.169384646432253 25.107747002573767 64-65 22.878717928161294 25.17437183290161 26.598926814973723 25.347983423963377 66-67 22.797579389981948 25.388898257525703 26.102005103296133 25.71151724919622 68-69 23.045418229769965 25.318403105104125 25.694791928719724 25.941386736406187 70-71 23.102505480652535 25.41986083427557 25.773857080063138 25.70377660500875 72-73 23.020122910371636 25.484412277767486 25.74593547067263 25.749529341188243 74-75 23.146599507363288 25.61835306736849 25.242931824276322 25.992115600991912 76-77 23.114392898511863 25.819609816242632 25.13221296723772 25.93378431800778 78-79 23.01929355563726 26.42420941759946 24.74628656417685 25.810210462586426 80-81 22.974784851559324 26.074221719694684 25.161240382940726 25.789753045805263 82-83 23.07181935548079 25.933646092218716 25.291034398869865 25.703500153430625 84-85 23.416830924979333 26.07228655864782 25.121431355690895 25.389451160681954 86-87 23.530590749377293 26.204983316147263 25.03020233491003 25.234223599565418 88-89 23.747190560837318 25.286334722041758 25.14202699826112 25.824447718859805 90-91 23.822108938508876 25.39898874012722 24.831848327606178 25.947053993757724 92-93 23.451387372244813 25.303751171463563 25.351024391322735 25.893837064968885 94-95 23.70917846884529 24.88313009534815 25.18100667077658 26.226684765029983 96-97 24.166705830640232 25.17437183290161 24.89031783637937 25.76860450007879 98-99 23.702958308337504 25.237264566924782 25.18598279918281 25.873794325554904 100-101 23.688997503642252 24.918239445769878 25.47874502041595 25.914018030171924 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.5 11 2.5 12 4.5 13 8.5 14 14.5 15 22.0 16 40.0 17 71.0 18 124.0 19 194.0 20 281.5 21 412.0 22 596.5 23 859.5 24 1178.5 25 1539.5 26 2040.5 27 2479.0 28 2964.0 29 3594.0 30 4159.5 31 4793.5 32 5521.5 33 5920.5 34 5986.5 35 6186.5 36 6951.5 37 7428.5 38 7594.0 39 8105.0 40 8633.5 41 8894.5 42 9119.5 43 9826.5 44 10372.5 45 10657.0 46 10945.0 47 11203.0 48 11489.0 49 11869.0 50 12275.0 51 12649.5 52 12942.0 53 12882.5 54 12484.5 55 11924.0 56 11062.5 57 10039.0 58 9066.0 59 8100.0 60 7369.0 61 6764.0 62 6115.0 63 5642.5 64 5235.0 65 4895.0 66 4633.0 67 4339.5 68 3943.0 69 3449.0 70 3043.5 71 2643.5 72 2183.0 73 1701.0 74 1288.0 75 980.0 76 694.5 77 469.5 78 312.0 79 199.0 80 125.0 81 73.0 82 46.0 83 26.5 84 16.0 85 11.0 86 4.5 87 2.0 88 2.5 89 3.0 90 4.5 91 2.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.056396121937262074 2 0.004146773671857506 3 0.008293547343715011 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 361727.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.07697678694475 #Duplication Level Percentage of deduplicated Percentage of total 1 96.27010563849223 89.60530387790661 2 2.7709344731083876 5.158204072633088 3 0.4317761343679801 1.205652517071757 4 0.17234148358398457 0.6416409706789661 5 0.10037314262045852 0.46712143328585565 6 0.06577770275024052 0.3673433827189614 7 0.03868085513887892 0.25202079391024246 8 0.02881514314576333 0.21456211277525628 9 0.02751244450488707 0.23046976426801313 >10 0.08794459956709026 1.402602366924645 >50 0.004145219407621412 0.27104274427505765 >100 0.0015931633124869277 0.18403596355152915 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-11 0.0 0.0 0.0 12-13 0.0 0.0 0.0 14-15 0.0 0.0 0.0 16-17 0.0 0.0 0.0 18-19 0.0 0.0 1.3822578906191687E-4 20-21 0.0 0.0 4.1467736718575057E-4 22-23 0.0 0.0 0.0015204836796810855 24-25 0.0 0.0 0.0027645157812383374 26-27 0.0 0.0 0.008155321554653094 28-29 0.0 0.0 0.03552402778891263 30-31 0.0 0.0 0.0623398308669245 32-33 0.0 0.0 0.0950993428745988 34-35 0.0 0.0 0.1730586879055199 36-37 0.0 0.0 0.35703721314693126 38-39 0.0 0.0 0.7037074921142188 40-41 0.0 0.0 1.0995861519875487 42-43 0.0 0.0 1.5893201226339202 44-45 0.0 0.0 2.2514216522405017 46-47 0.0 0.0 2.9918972042451903 48-49 0.0 0.0 3.659251313836125 50-51 0.0 0.0 4.352177194403515 52-53 0.0 0.0 5.031971625010021 54-55 0.0 0.0 5.77825266015531 56-57 0.0 0.0 6.6109248134642975 58-59 0.0 0.0 7.391485844296942 60-61 0.0 0.0 8.152418813082795 62-63 0.0 0.0 8.950949196493488 64-65 0.0 0.0 9.7984115092321 66-67 0.0 0.0 10.566255767471048 68-69 0.0 0.0 11.320692124170991 70-71 0.0 0.0 12.122954603886356 72-73 0.0 0.0 12.830117740727124 74-75 0.0 0.0 13.515579428685168 76-77 0.0 0.0 14.21375788923691 78-79 0.0 0.0 14.890511352484054 80-81 0.0 0.0 15.563394493637468 82-83 0.0 0.0 16.23199263532996 84-85 0.0 0.0 16.893679487569354 86-87 0.0 0.0 17.570294725027438 88-89 0.0 0.0 18.32113721121177 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAC 25 0.0050076405 57.031204 1 TAGGGAG 50 2.8232203E-5 47.499725 5 GGGAGTG 100 3.0211595E-8 37.99978 7 CCGTACG 50 0.0016143982 37.99978 9 TACACTC 100 1.2959899E-6 33.249805 5 CTAGCTA 175 3.6379788E-12 32.57124 6 TGGACCG 105 1.8969986E-6 31.666483 5 TCGTAGG 75 3.0744146E-4 31.666483 2 CTAAGAC 60 0.0039475174 31.666481 3 TCTAAGA 60 0.0039475174 31.666481 2 GTCCTAT 65 0.005823249 29.246773 1 ATGGACT 65 0.005839013 29.2306 4 CTTAGAC 65 0.005839013 29.2306 3 TGGACTG 115 3.8551207E-6 28.912874 5 TCTAGCT 200 2.3646862E-11 28.499834 5 ATACCGT 135 4.2118336E-7 28.147985 6 CTTTACA 85 6.398845E-4 27.941013 2 TTCTAGC 215 5.820766E-11 26.511473 4 GTCGTAG 90 8.907135E-4 26.403334 1 ATTCTAG 90 8.907135E-4 26.403334 1 >>END_MODULE