FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L93_CAGAGAGG-ACTGCATA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L93_CAGAGAGG-ACTGCATA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361727
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC250.005007640557.0312041
TAGGGAG502.8232203E-547.4997255
GGGAGTG1003.0211595E-837.999787
CCGTACG500.001614398237.999789
TACACTC1001.2959899E-633.2498055
CTAGCTA1753.6379788E-1232.571246
TGGACCG1051.8969986E-631.6664835
TCGTAGG753.0744146E-431.6664832
CTAAGAC600.003947517431.6664813
TCTAAGA600.003947517431.6664812
GTCCTAT650.00582324929.2467731
ATGGACT650.00583901329.23064
CTTAGAC650.00583901329.23063
TGGACTG1153.8551207E-628.9128745
TCTAGCT2002.3646862E-1128.4998345
ATACCGT1354.2118336E-728.1479856
CTTTACA856.398845E-427.9410132
TTCTAGC2155.820766E-1126.5114734
GTCGTAG908.907135E-426.4033341
ATTCTAG908.907135E-426.4033341
Produced by FastQC (version 0.11.2)