FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L92_CTCTCTAC-ACTGCATA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L92_CTCTCTAC-ACTGCATA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2496732
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT8500.026.8163851
GTATATA5850.025.1642381
CCTAGGG1000.001655787123.7460562
TATCCCG9800.022.2922125
TAGGGAG3450.022.0253265
ATCCCGT9700.021.0536256
ATTATCC11800.020.9287243
ATAATAC10350.020.648743
GTATAGA4950.020.1461491
CTATACT2851.9717845E-919.9966754
TCTAGAG1908.269482E-619.9966753
GTATAAT5300.019.711731
GTATACG3707.2759576E-1219.2516271
TATTATC11650.019.1599062
GTGTTAT8000.018.994941
GTATAAG3609.640644E-1118.4673021
TATATAC6950.018.450173
TGCTATA2854.2862666E-818.3302862
TTATCCC12350.018.073924
TATAGGG8000.017.8095422
Produced by FastQC (version 0.11.2)