FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L92_CTCTCTAC-ACTGCATA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L92_CTCTCTAC-ACTGCATA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2496732
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG2900.027.8640961
CTATACT2551.8189894E-1124.2154584
GTATTAT11200.024.1908341
GTCCTAC3550.024.1011731
CCTAGCA1201.7105478E-423.7521512
TACACAG3800.023.7497755
GTATAGA4900.023.2814041
TACACTG5600.022.901575
GCCCTAT7100.022.7622181
ATCCCGT10400.021.9228676
TATCCCG10450.021.8179765
CTATAGT7850.021.7832344
ATAATAC10500.021.2638323
ATAGGGT4400.020.5132243
GTCCTAT3052.0736479E-1020.259911
ATTATCC11950.019.4787543
ATAGAAC5900.019.3237863
TAATACC10350.019.2751794
GTGCTAC7900.019.2538191
CCCTATA8150.019.2348732
Produced by FastQC (version 0.11.2)