FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L87_GGACTCCT-ACTGCATA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L87_GGACTCCT-ACTGCATA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359554
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATC459.6280227E-442.2117164
ATAGGGC958.694733E-734.991293
CTATACT550.002580426234.5368544
GTATGCT550.002580426234.5368544
TATACAG702.0535364E-433.9201285
TTAAGAG600.00395218431.6587873
TCTATAC753.0787638E-431.6587873
CACCTCA650.005841933729.2275587
TATAGGG1303.0336741E-729.2234952
CACTACA1004.952169E-528.4929072
CTTGCAC1004.952169E-528.4929073
CAGGAGT1004.952169E-528.4929074
CCACTAA700.00839422127.1361036
TACACTG1056.904548E-527.13615
GTATTAA1257.3867013E-626.593381
ATAGGGA1301.0015421E-525.5705593
ACTGGAC1701.1210432E-725.14083
TAAACCC1151.2815239E-424.776444
CTATAGG1551.4091875E-624.5100271
CAGGACA1201.7105158E-423.7440914
Produced by FastQC (version 0.11.2)