##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename G140_A6_L87_GGACTCCT-ACTGCATA_L006_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 359554 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.72587705880063 33.0 33.0 33.0 33.0 33.0 2 32.76399372556 33.0 33.0 33.0 33.0 33.0 3 32.77748265907207 33.0 33.0 33.0 33.0 33.0 4 36.73981933172764 37.0 37.0 37.0 37.0 37.0 5 36.62598942022617 37.0 37.0 37.0 37.0 37.0 6 36.62971625958827 37.0 37.0 37.0 37.0 37.0 7 36.58572564899848 37.0 37.0 37.0 37.0 37.0 8 36.594575501871766 37.0 37.0 37.0 37.0 37.0 9 36.64057137453623 37.0 37.0 37.0 37.0 37.0 10-11 36.61420676727279 37.0 37.0 37.0 37.0 37.0 12-13 36.589698626631886 37.0 37.0 37.0 37.0 37.0 14-15 38.85526235280375 40.0 38.5 40.0 37.0 40.0 16-17 38.78509625814203 40.0 37.0 40.0 37.0 40.0 18-19 38.71127563592673 40.0 37.0 40.0 37.0 40.0 20-21 38.6669540597518 40.0 37.0 40.0 37.0 40.0 22-23 38.607775188149766 40.0 37.0 40.0 37.0 40.0 24-25 38.533075699338625 40.0 37.0 40.0 37.0 40.0 26-27 38.426137659433635 40.0 37.0 40.0 37.0 40.0 28-29 38.31980314500743 40.0 37.0 40.0 37.0 40.0 30-31 38.1883444489562 40.0 37.0 40.0 37.0 40.0 32-33 38.01678329263476 40.0 37.0 40.0 35.0 40.0 34-35 37.84401647596745 40.0 37.0 40.0 33.0 40.0 36-37 37.66696379403372 40.0 37.0 40.0 33.0 40.0 38-39 37.44745851805292 40.0 37.0 40.0 33.0 40.0 40-41 37.223818953481256 38.5 37.0 40.0 33.0 40.0 42-43 36.96322110169822 37.0 37.0 40.0 33.0 40.0 44-45 36.66989103166701 37.0 37.0 40.0 33.0 40.0 46-47 36.42756303642847 37.0 37.0 40.0 33.0 40.0 48-49 36.37390489328446 37.0 37.0 40.0 33.0 40.0 50-51 36.62864966041262 37.0 37.0 40.0 33.0 40.0 52-53 36.54496125755797 37.0 37.0 40.0 33.0 40.0 54-55 36.34426678607386 37.0 37.0 40.0 33.0 40.0 56-57 36.156749751080504 37.0 37.0 40.0 33.0 40.0 58-59 35.96913120143289 37.0 37.0 38.5 33.0 40.0 60-61 35.79653960184005 37.0 35.0 37.0 33.0 40.0 62-63 35.589655517669115 37.0 33.0 37.0 33.0 40.0 64-65 35.41993275001808 37.0 33.0 37.0 33.0 40.0 66-67 35.22224756225769 37.0 33.0 37.0 33.0 40.0 68-69 35.0388648158552 37.0 33.0 37.0 33.0 40.0 70-71 34.86393976982595 37.0 33.0 37.0 30.0 38.5 72-73 34.66976865783721 37.0 33.0 37.0 27.0 37.0 74-75 34.47389960895999 37.0 33.0 37.0 27.0 37.0 76-77 33.22269533922582 33.0 33.0 37.0 27.0 37.0 78-79 34.37794740150297 37.0 33.0 37.0 27.0 37.0 80-81 34.468809970129655 37.0 33.0 37.0 27.0 37.0 82-83 34.380926091769254 37.0 33.0 37.0 27.0 37.0 84-85 34.30303653971309 37.0 33.0 37.0 27.0 37.0 86-87 34.21933005890631 37.0 33.0 37.0 27.0 37.0 88-89 34.113606023017404 37.0 33.0 37.0 27.0 37.0 90-91 33.9996342691223 37.0 33.0 37.0 27.0 37.0 92-93 33.920579384459636 37.0 33.0 37.0 27.0 37.0 94-95 33.87476568192817 37.0 33.0 37.0 27.0 37.0 96-97 33.76246822452261 37.0 33.0 37.0 27.0 37.0 98-99 33.656791469431575 37.0 33.0 37.0 27.0 37.0 100-101 32.76682918282094 35.0 33.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.0472803972053768 2306 2 0.006351919178499088 2306 3 0.16379106021250323 2306 4 0.020884969054641545 2306 5 0.18675312880238693 2306 6 0.1013388698473392 2306 7 0.14575198607650464 2306 8 0.09286085513736708 2306 9 0.10062073506982472 2306 10-11 0.13762540304696813 2306 12-13 0.11426809731509735 2306 14-15 0.4675965135950335 2306 16-17 0.5386381209876134 2306 18-19 0.5476217310258988 2306 20-21 0.44084949810937957 2306 22-23 0.2918911869762155 2306 24-25 0.35808098761904006 2306 26-27 0.5335087548026678 2306 28-29 0.48396414945731436 2306 30-31 0.607199518793756 2306 32-33 0.6719264535175 2306 34-35 0.2471832932715614 2306 36-37 0.24263193513166215 2306 38-39 0.3193356746058029 2306 40-41 0.45304179438385006 2306 42-43 0.4507728891817919 2306 44-45 0.5669417317225083 2306 46-47 0.3898777577132151 2306 48-49 0.13054295225826706 2306 50-51 0.3471685100640727 2306 52-53 0.296180946111825 2306 54-55 0.38664512879510227 2306 56-57 0.15546663881866607 2306 58-59 -0.1433337257292706 2306 60-61 0.351762183103304 2306 62-63 0.35672923159109615 2306 64-65 0.19647584224114212 2306 66-67 0.05297898570376702 2306 68-69 -0.301157095242381 2306 70-71 0.30017350505688256 2306 72-73 0.2791623780784036 2306 74-75 0.5607621294109819 2306 76-77 0.2119195465260688 2306 78-79 0.23780895325948848 2306 80-81 0.5883910026119139 2306 82-83 0.6434678054820395 2306 84-85 0.6269602377207733 2306 86-87 0.45688511268985366 2306 88-89 0.6238033758572357 2306 90-91 0.5260055636688534 2306 92-93 0.4080177068106039 2306 94-95 0.4815110621047367 2306 96-97 0.32976293346374774 2306 98-99 0.36139280271284946 2306 100-101 0.22887874032090139 2307 1 -0.07573284782773726 2307 2 -0.1159162927420283 2307 3 0.10977615955025044 2307 4 0.1364892736904011 2307 5 0.09681107582225934 2307 6 0.07762198243012364 2307 7 0.23546639005001424 2307 8 0.18481035844862248 2307 9 0.08865881454002533 2307 10-11 0.14753848251717017 2307 12-13 0.035698146983968115 2307 14-15 0.1369156361115884 2307 16-17 0.17339598522602984 2307 18-19 0.18601163168815305 2307 20-21 0.045526650427262894 2307 22-23 -0.02278182626881886 2307 24-25 0.26336865318194924 2307 26-27 0.33791686738545224 2307 28-29 0.39906141766923753 2307 30-31 0.31679182011826157 2307 32-33 0.5117546819943328 2307 34-35 0.43359678664904777 2307 36-37 0.16841835897272261 2307 38-39 0.35294494612897864 2307 40-41 0.24149378113882136 2307 42-43 0.03582048851954056 2307 44-45 0.21148850324568969 2307 46-47 0.2294162510907043 2307 48-49 0.24246348205959833 2307 50-51 0.030539784898515165 2307 52-53 0.1216362441250638 2307 54-55 0.056585112238813906 2307 56-57 -0.19422293071775698 2307 58-59 -0.261048957517346 2307 60-61 -0.35479824736027155 2307 62-63 -0.08783823529632429 2307 64-65 -0.3048383133879966 2307 66-67 -0.2080930341637881 2307 68-69 0.0763366961483456 2307 70-71 -0.14582194196297849 2307 72-73 -0.09581692655736163 2307 74-75 -0.056559890456576056 2307 76-77 0.11746590414195879 2307 78-79 0.25720050955087004 2307 80-81 0.038887691353636455 2307 82-83 -0.19481861835901526 2307 84-85 0.32982654566779246 2307 86-87 0.1591202120275952 2307 88-89 0.0645939387711465 2307 90-91 0.34515912327150744 2307 92-93 0.10867995846623302 2307 94-95 -0.1963448981601701 2307 96-97 -0.2771286228276395 2307 98-99 -0.04662665092953944 2307 100-101 -0.015171342460554627 2201 1 0.06938910339177795 2201 2 0.03865687798968054 2201 3 0.02364811076976281 2201 4 0.07810512734894104 2201 5 0.042390344114942025 2201 6 0.142103689747195 2201 7 0.19392675590367503 2201 8 0.06491706576569811 2201 9 0.14338442429612286 2201 10-11 0.18343787276106838 2201 12-13 0.1295624762522678 2201 14-15 0.12727386781890004 2201 16-17 0.25140665595773726 2201 18-19 0.17099638778571347 2201 20-21 0.2560449431101901 2201 22-23 0.4006175639750822 2201 24-25 0.21089151903561287 2201 26-27 0.26024918445862255 2201 28-29 0.23576111279118805 2201 30-31 0.20303419816703894 2201 32-33 0.0502074258967653 2201 34-35 0.17044892079538698 2201 36-37 -0.11466090931995865 2201 38-39 -0.02540871240761078 2201 40-41 -0.017500121300201954 2201 42-43 0.04538603730002677 2201 44-45 -0.16499777724211384 2201 46-47 -8.810050068603914E-4 2201 48-49 0.020817140596172123 2201 50-51 0.10557789465460843 2201 52-53 0.07697160997872743 2201 54-55 0.3236201732144153 2201 56-57 0.2102740205017568 2201 58-59 0.21517055467777624 2201 60-61 0.2725645575177751 2201 62-63 0.3745474354353817 2201 64-65 0.31318760124614897 2201 66-67 0.310580746324014 2201 68-69 0.05381383029469333 2201 70-71 0.19301189950044062 2201 72-73 0.26310075636946806 2201 74-75 0.24061998759220415 2201 76-77 0.2747829773126895 2201 78-79 0.32678892418502414 2201 80-81 0.42242427671948946 2201 82-83 0.24618747920195716 2201 84-85 0.369651240789743 2201 86-87 0.26033972422272456 2201 88-89 0.2910421095028468 2201 90-91 0.314175891564183 2201 92-93 0.5480092267589214 2201 94-95 0.14008802866910486 2201 96-97 0.08072198692845944 2201 98-99 -0.09384463873441717 2201 100-101 0.270461279490668 1101 1 -0.04290152252652746 1101 2 0.07700539400495643 1101 3 0.007743027020133297 1101 4 -0.055453497393934015 1101 5 -0.06214976755124013 1101 6 -0.01808584889518272 1101 7 -0.04446583886564781 1101 8 -0.08171825600921068 1101 9 -0.10433817341178298 1101 10-11 -0.03871904760331546 1101 12-13 0.05582239397192268 1101 14-15 -0.11231252653902146 1101 16-17 -0.09729302079806956 1101 18-19 -0.1306675851793173 1101 20-21 -0.09430768692212865 1101 22-23 -0.26431044072665344 1101 24-25 -0.019970955251785938 1101 26-27 0.13396355413243555 1101 28-29 0.03883927911502383 1101 30-31 6.208863833236933E-4 1101 32-33 0.05518465187384436 1101 34-35 -0.31028499045937963 1101 36-37 -0.22698826753330792 1101 38-39 -0.06091047555776896 1101 40-41 -0.20255742368045304 1101 42-43 -0.2519618909985297 1101 44-45 0.24693654541436416 1101 46-47 -0.2880724537285744 1101 48-49 -0.26446422878378684 1101 50-51 0.1643312005611577 1101 52-53 0.06598865376361829 1101 54-55 -0.15612196607444417 1101 56-57 0.0128930331376651 1101 58-59 0.1359389942898801 1101 60-61 0.2910225357722567 1101 62-63 0.06274534904103035 1101 64-65 -0.057643283267516665 1101 66-67 0.1320801586072946 1101 68-69 0.2093750997628021 1101 70-71 0.02799206406111665 1101 72-73 0.24254903729806188 1101 74-75 0.058538001109688764 1101 76-77 0.26249602462389277 1101 78-79 0.49887972641834466 1101 80-81 0.25033347448616894 1101 82-83 0.22453379127953355 1101 84-85 0.10044025048707539 1101 86-87 -0.02747617351456455 1101 88-89 0.17276411949403325 1101 90-91 0.24201529797030474 1101 92-93 0.14452333196020817 1101 94-95 0.3338508849723567 1101 96-97 0.37441505187115354 1101 98-99 0.34198404184154185 1101 100-101 0.4698625430816108 2304 1 0.056388813893335055 2304 2 0.025738009928595318 2304 3 0.03141933462443092 2304 4 0.06173037161325112 2304 5 0.048053657424631524 2304 6 -0.03938469412774026 2304 7 0.10467076690461141 2304 8 0.03810227239521424 2304 9 0.03567290949551705 2304 10-11 0.09344909082577146 2304 12-13 0.15416532057447796 2304 14-15 0.16556456044393286 2304 16-17 0.17691508759991592 2304 18-19 0.12732283346856832 2304 20-21 0.31364608662904203 2304 22-23 0.2846643903483894 2304 24-25 0.21213998107513277 2304 26-27 0.05079706026378261 2304 28-29 0.06706494140811259 2304 30-31 0.2985008334713726 2304 32-33 0.1191035321426952 2304 34-35 0.15046900474993663 2304 36-37 0.10148586639112267 2304 38-39 0.07393901141087866 2304 40-41 -0.060861560107468904 2304 42-43 -0.016910409106571933 2304 44-45 0.11679729404687578 2304 46-47 0.1618578312093959 2304 48-49 -0.09448013811844902 2304 50-51 0.11526252228723166 2304 52-53 0.34565330643960834 2304 54-55 0.5172629683218943 2304 56-57 0.5042099327837306 2304 58-59 0.32404006325416645 2304 60-61 0.27453224225396866 2304 62-63 0.10142827063840087 2304 64-65 0.36508472073662546 2304 66-67 0.10853344951573973 2304 68-69 -0.05685599672999331 2304 70-71 -0.03590447163657018 2304 72-73 -0.09625275741789352 2304 74-75 -0.225671537340844 2304 76-77 -0.2806424044633786 2304 78-79 0.12664598432832008 2304 80-81 0.27946632636847113 2304 82-83 0.05464354781309311 2304 84-85 0.003254772670764794 2304 86-87 0.13682792716112857 2304 88-89 0.3549036568720325 2304 90-91 0.040351074310081 2304 92-93 -0.1579372522162572 2304 94-95 -0.36401923050438256 2304 96-97 -0.23043908276829228 2304 98-99 -0.08697809751708263 2304 100-101 -0.052008323172721305 2202 1 0.17847069199527255 2202 2 0.10063975445561368 2202 3 0.02948486161514552 2202 4 0.10999591085854377 2202 5 -0.0975657678355617 2202 6 0.16348112697289707 2202 7 -0.10888279874054518 2202 8 0.01365438499729521 2202 9 0.1200194340090519 2202 10-11 0.04640463295964281 2202 12-13 0.09505010126125768 2202 14-15 0.14757658743901914 2202 16-17 0.2389057566124606 2202 18-19 0.16496627770585093 2202 20-21 0.1631335825806559 2202 22-23 0.09175541561908318 2202 24-25 0.055574589760119864 2202 26-27 0.0654150237276312 2202 28-29 0.0881515725364963 2202 30-31 0.20840778619496092 2202 32-33 -0.08240553186998056 2202 34-35 -0.03983149506186123 2202 36-37 0.105678683456496 2202 38-39 0.2049360965714513 2202 40-41 0.05010366314472492 2202 42-43 0.282543386749623 2202 44-45 0.5307132451335903 2202 46-47 0.1891461552204916 2202 48-49 0.29747823132213114 2202 50-51 0.35267750613744653 2202 52-53 0.4655337367504373 2202 54-55 0.48400491312376204 2202 56-57 0.4677700633825381 2202 58-59 0.3325351132791141 2202 60-61 0.4568628883329424 2202 62-63 0.3360509608688673 2202 64-65 0.30266076478309145 2202 66-67 0.07689037291586232 2202 68-69 0.01702161502740296 2202 70-71 0.31900567603111796 2202 72-73 0.5196974539736132 2202 74-75 0.38777086175581843 2202 76-77 0.3773847831980106 2202 78-79 0.0941013207603163 2202 80-81 0.3427962459259035 2202 82-83 0.3512175173341987 2202 84-85 0.24600925215747083 2202 86-87 0.5401305365113771 2202 88-89 0.46253826030506673 2202 90-91 0.5339174197316794 2202 92-93 0.3256416103836344 2202 94-95 0.23899802219381883 2202 96-97 -0.17918430129371643 2202 98-99 -0.08451842821568079 2202 100-101 0.24591365016481603 1102 1 -0.06197204091130715 1102 2 0.002563534347309826 1102 3 -0.003966635838800414 1102 4 -0.016626720545907858 1102 5 0.094667704064328 1102 6 0.12735180951946035 1102 7 0.03558346497796805 1102 8 -0.35509418045627683 1102 9 -0.030703577390809755 1102 10-11 0.04520598972177936 1102 12-13 -0.035172710364726356 1102 14-15 -0.23642280048783704 1102 16-17 -0.08511896434169586 1102 18-19 0.047511991918696594 1102 20-21 -0.1405864619646664 1102 22-23 -0.10144717209014686 1102 24-25 0.1260352598101946 1102 26-27 -0.13716418448775158 1102 28-29 -0.1080306212356632 1102 30-31 -0.1314925818990318 1102 32-33 0.038245135884814374 1102 34-35 -0.13068185167948343 1102 36-37 0.10179371920326474 1102 38-39 -0.09885620660877947 1102 40-41 -0.12113229955281923 1102 42-43 -0.0772153544199412 1102 44-45 -0.24029914084653115 1102 46-47 -0.22213724314561034 1102 48-49 -0.3585451634735435 1102 50-51 -0.1573069370035043 1102 52-53 -0.18259646480864689 1102 54-55 0.006103303881459965 1102 56-57 -0.13344303158230986 1102 58-59 0.05141501942789972 1102 60-61 -0.026475120559119603 1102 62-63 -0.014751960080182869 1102 64-65 0.001082976237363198 1102 66-67 -0.24199973445197287 1102 68-69 -0.3833039738804729 1102 70-71 -0.25615605579583445 1102 72-73 0.1862706094656943 1102 74-75 0.13682087323218894 1102 76-77 0.23335207128894098 1102 78-79 0.49937450090534696 1102 80-81 0.34674071729023836 1102 82-83 0.1589457908628802 1102 84-85 0.16598918976000476 1102 86-87 0.23797467888639545 1102 88-89 0.2653954517394439 1102 90-91 0.334572848055366 1102 92-93 0.4952793820973582 1102 94-95 0.29192959751706127 1102 96-97 0.43779392760463764 1102 98-99 0.39189550324913824 1102 100-101 0.4724232180581751 2305 1 0.11328981506178337 2305 2 0.08843923133728993 2305 3 -0.017437013254280487 2305 4 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-2.2431415867432705 1213 86-87 -0.9875695112925555 1213 88-89 -1.8634816541142314 1213 90-91 -2.2962008075585345 1213 92-93 -1.444284468411233 1213 94-95 -1.0448873882787453 1213 96-97 -1.2729907769778421 1213 98-99 -0.628562174644955 1213 100-101 -0.35166467250007827 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 17.0 15 37.0 16 66.0 17 113.0 18 200.0 19 293.0 20 384.0 21 556.0 22 768.0 23 984.0 24 1271.0 25 1595.0 26 2067.0 27 2650.0 28 3369.0 29 4240.0 30 5487.0 31 7385.0 32 9738.0 33 13585.0 34 20287.0 35 34525.0 36 73254.0 37 121637.0 38 54992.0 39 51.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.55601382603904 18.52321872860554 9.368198996999906 25.552568448355512 2 19.123933703993167 18.32596741938983 34.85769276768306 27.69240610893394 3 22.418310470278026 22.642768932946165 28.216959825550997 26.72196077122481 4 14.106365107883656 14.731027884545856 36.22320986555566 34.939397142014826 5 15.010262714362794 34.372027567486384 34.279969072795744 16.33774064535508 6 33.76377400891104 35.39996773780851 16.432024118769366 14.404234134511087 7 27.61782652953381 28.389616024296764 21.88405635871107 22.10850108745835 8 27.135562391184635 33.258147593963635 19.268593869071125 20.33769614578061 9 23.870406114241533 13.573482703571646 19.93914683190842 42.6169643502784 10-11 28.781629463168258 22.149663193845708 26.43233005334387 22.63637728964217 12-13 26.619784510810618 20.079876736178708 26.871763351262956 26.42857540174772 14-15 25.093449106392917 21.515266135267584 21.707309611351842 31.68397514698766 16-17 25.66095774209159 25.922114619778945 23.107099350862455 25.309828287267006 18-19 25.355579412271872 25.338753010674335 24.580174327083 24.725493249970796 20-21 25.64121105591928 24.024068707342984 24.51231247601195 25.822407760725792 22-23 25.928372372439192 23.771672683379965 24.557090172825223 25.742864771355624 24-25 25.833393593173763 24.312481574394944 24.11167724458635 25.742447587844943 26-27 25.287161316519914 24.6483143004945 24.37658877386985 25.687935609115737 28-29 25.572792960167316 24.293152071733314 24.383819954721684 25.750235013377686 30-31 25.528988691545635 24.47448783771005 24.454602090367512 25.5419213803768 32-33 25.491581236754424 24.645394015919724 24.40746035366037 25.455564393665487 34-35 25.85925897083609 24.19761704778698 24.374085672805755 25.569038308571173 36-37 25.73340861178015 24.254910249920737 24.559315151548862 25.45236598675025 38-39 25.287161316519914 24.2380838483232 24.729108840396712 25.745645994760174 40-41 25.440267664940453 23.974562930741975 24.429153896215865 26.156015508101703 42-43 25.588367811232803 23.697692140818905 24.690032651562767 26.02390739638552 44-45 25.227086890981603 23.69157344932889 25.101792776606572 25.979546883082932 46-47 25.275480178220793 23.421794779087424 25.569455492081854 25.733269550609922 48-49 24.906968077117764 23.808106709979583 25.21582293619317 26.069102276709476 50-51 24.539846587717005 24.12349744405569 25.385755686211255 25.950900282016054 52-53 24.132953603631165 24.25240714885664 25.499368662287168 26.11527058522503 54-55 24.2421166222598 24.021148422768206 25.513552901650378 26.223182053321615 56-57 23.844679797749436 24.28884117545626 25.505209231436726 26.361269795357583 58-59 24.022399973300253 24.292317704711948 25.373657364401453 26.311624957586343 60-61 24.332506382907713 24.06648236426239 25.189818497360616 26.411192755469276 62-63 23.968861422762643 23.443349260472697 25.48935625803078 27.098433058733878 64-65 24.10347263554292 23.72522625252396 24.959394138293554 27.211906973639564 66-67 24.22236993608749 23.786969412104998 24.560844824421366 27.42981582738615 68-69 24.428597651534957 23.895576186052722 24.082752521179014 27.593073641233307 70-71 24.283278728647158 24.05966836692124 24.085255622243114 27.571797282188488 72-73 24.419697736640394 23.84440167540898 24.073574483943997 27.66232610400663 74-75 24.474348776539827 23.948836614249878 23.8414813908342 27.735333218376102 76-77 24.46308482175139 24.202345127574716 23.768474276464733 27.56609577420916 78-79 24.446258420153857 24.14477380310051 23.53665930569539 27.872308471050246 80-81 24.18384999193445 24.000706430744756 23.711181074330977 28.104262502989812 82-83 23.8253502950878 23.646934813685842 24.212913776512014 28.31480111471434 84-85 23.859420281793557 23.702698342947095 24.240586949387296 28.197294425872055 86-87 23.94814130839874 23.950366287122378 24.3521140079098 27.749378396569085 88-89 24.241977561089573 23.31624735088471 24.364351390889823 28.077423697135895 90-91 24.367688858975285 23.09416666203129 24.183293747253543 28.354850731739877 92-93 23.92853368339665 23.04062811149368 24.720069864331922 28.31076834077774 94-95 23.98054256106176 22.738587249759423 24.781813023912957 28.499057165265857 96-97 24.48269244675348 22.84302218860032 24.4918704839885 28.182414880657703 98-99 23.91226352648003 22.62261023378964 25.03087157979052 28.43425465993981 100-101 24.161043960017132 22.408456031639197 25.249892922898926 28.18060708544475 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 1.0 11 3.0 12 7.0 13 14.5 14 20.5 15 30.0 16 40.0 17 61.5 18 100.5 19 169.5 20 265.5 21 414.0 22 593.0 23 769.0 24 1003.0 25 1275.5 26 1608.0 27 2018.5 28 2429.5 29 2866.5 30 3350.0 31 3925.5 32 4464.0 33 4934.0 34 5121.0 35 5155.0 36 5730.0 37 6311.5 38 6609.5 39 6985.0 40 7346.0 41 7457.5 42 7518.5 43 7842.0 44 8174.0 45 8448.0 46 8576.5 47 8801.0 48 9112.0 49 9355.0 50 9426.0 51 9328.5 52 9486.5 53 9565.5 54 9569.0 55 9467.5 56 9395.5 57 9475.5 58 9394.5 59 9416.0 60 9575.0 61 9534.5 62 9450.0 63 9440.5 64 9380.0 65 9306.0 66 9043.0 67 8664.0 68 8068.5 69 7273.5 70 6357.0 71 5256.0 72 4245.0 73 3394.0 74 2537.0 75 1748.0 76 1156.5 77 718.0 78 415.5 79 235.5 80 134.0 81 69.5 82 47.0 83 32.0 84 17.0 85 9.5 86 5.5 87 3.0 88 2.0 89 1.5 90 1.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0645243829855877 2 0.004171835106826791 3 0.006118691490012628 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 359554.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.84147290392875 #Duplication Level Percentage of deduplicated Percentage of total 1 96.91039508179293 89.97303819095308 2 2.251740687761148 4.181098440989009 3 0.2545400996188754 0.7089563328518743 4 0.1620302730345996 0.6017251681423184 5 0.10876848749768087 0.5049113292408626 6 0.04935237469124769 0.2749168294585207 7 0.06517856744731115 0.4235891942503502 8 0.03423818184646089 0.2542978585742387 9 0.014575472425509125 0.12178874954293796 >10 0.14101855761843043 2.362553100597304 >50 0.007218272981946303 0.47492429752735893 >100 9.439432838679873E-4 0.11820050787210809 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-11 2.781223404551194E-4 0.0 0.0 12-13 2.781223404551194E-4 0.0 0.0 14-15 2.781223404551194E-4 0.0 0.0 16-17 2.781223404551194E-4 0.0 0.0 18-19 2.781223404551194E-4 0.0 2.781223404551194E-4 20-21 2.781223404551194E-4 0.0 0.0015296728725031567 22-23 2.781223404551194E-4 0.0 0.002224978723640955 24-25 2.781223404551194E-4 0.0 0.003615590425916552 26-27 2.781223404551194E-4 0.0 0.01432330053343865 28-29 2.781223404551194E-4 0.0 0.052008877665107325 30-31 2.781223404551194E-4 0.0 0.0831585797960807 32-33 4.171835106826791E-4 0.0 0.11792387235297062 34-35 5.562446809102388E-4 0.0 0.22333223938546087 36-37 5.562446809102388E-4 0.0 0.5049311090962693 38-39 5.562446809102388E-4 0.0 0.9763484761676966 40-41 5.562446809102388E-4 0.0 1.5380165427168102 42-43 5.562446809102388E-4 0.0 2.194802449701575 44-45 5.562446809102388E-4 0.0 3.140279346078753 46-47 5.562446809102388E-4 0.0 4.2159174977889275 48-49 5.562446809102388E-4 0.0 5.076706141497522 50-51 5.562446809102388E-4 0.0 5.879089093710542 52-53 5.562446809102388E-4 0.0 6.6628378491130675 54-55 5.562446809102388E-4 0.0 7.578416593891321 56-57 5.562446809102388E-4 0.0 8.58368979346635 58-59 5.562446809102388E-4 0.0 9.492037357392771 60-61 5.562446809102388E-4 0.0 10.31444511811856 62-63 5.562446809102388E-4 0.0 11.10069697458518 64-65 5.562446809102388E-4 0.0 11.965101208719691 66-67 5.562446809102388E-4 0.0 12.865244163602686 68-69 5.562446809102388E-4 0.0 13.716993831246489 70-71 5.562446809102388E-4 0.0 14.544268732930242 72-73 5.562446809102388E-4 0.0 15.275313304816523 74-75 6.953058511377985E-4 0.0 16.02582644053466 76-77 8.343670213653582E-4 0.0 16.792887855509882 78-79 8.343670213653582E-4 0.0 17.5286605071839 80-81 8.343670213653582E-4 0.0 18.26026132375109 82-83 8.343670213653582E-4 0.0 18.932343959460887 84-85 0.0011124893618204776 0.0 19.60706875740501 86-87 0.0011124893618204776 0.0 20.32086974418307 88-89 0.0012515505320480373 0.0 21.06137047564483 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTAAGG 25 0.005018623 56.99958 3 GGGTATG 60 8.252633E-5 39.605072 1 TAAGAGT 55 0.0025773705 34.545204 4 GTATATG 105 1.8887549E-6 31.68406 1 CTTAACA 60 0.003944828 31.670837 2 ACCCTTA 60 0.0039475155 31.666435 6 CATGTAC 80 4.48776E-4 29.687283 3 CCAATAG 65 0.005839011 29.230556 3 TAGGGAG 65 0.005839011 29.230556 5 GATAGGG 85 6.3936244E-4 27.944857 2 ACTATAG 70 0.008361605 27.157766 1 ACTCTAC 70 0.008361605 27.157766 1 TCTTAAC 70 0.008361605 27.157766 1 TTAAGAC 70 0.008384335 27.14266 3 AGACCCC 70 0.008384335 27.14266 6 AGCACTC 70 0.008384335 27.14266 5 ATGTACT 90 8.9359266E-4 26.388695 4 AGTGAGT 180 6.666596E-9 26.388695 8 TAGGGTT 90 8.9359266E-4 26.388695 4 GTTCTAG 95 0.0012209996 25.013731 1 >>END_MODULE