FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L87_GGACTCCT-ACTGCATA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L87_GGACTCCT-ACTGCATA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359554
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAAGG250.00501862356.999583
GGGTATG608.252633E-539.6050721
TAAGAGT550.002577370534.5452044
GTATATG1051.8887549E-631.684061
CTTAACA600.00394482831.6708372
ACCCTTA600.003947515531.6664356
CATGTAC804.48776E-429.6872833
CCAATAG650.00583901129.2305563
TAGGGAG650.00583901129.2305565
GATAGGG856.3936244E-427.9448572
ACTATAG700.00836160527.1577661
ACTCTAC700.00836160527.1577661
TCTTAAC700.00836160527.1577661
TTAAGAC700.00838433527.142663
AGACCCC700.00838433527.142666
AGCACTC700.00838433527.142665
ATGTACT908.9359266E-426.3886954
AGTGAGT1806.666596E-926.3886958
TAGGGTT908.9359266E-426.3886954
GTTCTAG950.001220999625.0137311
Produced by FastQC (version 0.11.2)