FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L86_TCCTGAGC-ACTGCATA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L86_TCCTGAGC-ACTGCATA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3947640
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG10900.020.910585
ACTGTCC10700.019.0849518
GTGTAAG8500.018.4342021
TACACTG14250.018.327335
TAGACAG8700.018.0113415
TAGGACT5550.017.9670854
GTAGGAC5600.017.8066653
CTACACT12700.017.5729834
GTATAGG9350.017.2661951
GTGCTAC12200.016.7354721
CTTTATA28000.016.6187131
TCTATAC9750.016.5586763
GCTATAC8350.016.4916043
GTCCTAC5800.016.3731161
GTCCTAT4701.4551915E-1116.1640971
GTATAAG7700.016.0328941
GTATAGA10150.015.9053131
TACACCG19800.015.8281475
GTATATA14150.015.7714171
GTGTACG12100.015.6965391
Produced by FastQC (version 0.11.2)