FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L86_TCCTGAGC-ACTGCATA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L86_TCCTGAGC-ACTGCATA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3947640
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAG9700.022.525515
TCTATAC10200.019.5598353
TATACCG10900.019.174095
GTATAGA10400.018.7402231
TACACTG16850.018.6051255
GTGTAAG9750.018.526921
CTTACAC7750.018.3880483
TACACCG21750.018.3446145
TATACAG13000.017.9036395
CTACACT15000.017.7331284
GTAGGGC7450.017.2156563
GTACACT12750.017.1370564
ATAATAC17750.016.8600273
GTGTAGG11800.016.5168081
GTATTAT17050.016.4494691
GTAGTAC11900.016.3663923
ATAGGGT7000.016.2865563
GTCTTAG5550.016.2736451
TATTAGG7900.016.235842
TCTACAC18650.016.0464063
Produced by FastQC (version 0.11.2)