FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L85_AGGCAGAA-ACTGCATA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L85_AGGCAGAA-ACTGCATA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267486
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG5350.027.5121385
CTACACT5100.022.3438244
GTCCTAA2257.196104E-821.099171
TTACACT4750.020.9914344
CCCTTAC1856.585131E-620.528921
CTTACAC3102.6011548E-1019.911233
GTGTACG3353.45608E-1119.8395161
TAGACTG2401.4195575E-719.7835925
GTGCTAG1200.00477938219.7804721
GTGTAAG2902.4556357E-919.6440541
TAGCCCG1707.979649E-519.5508465
ACTATGC3759.094947E-1218.9967468
TACTGCG2803.497553E-818.6567847
CTATACA3609.640644E-1118.4646874
GTCCTAC1559.661908E-418.3766961
GTAGGAC1300.00758136118.2617783
TCTACAC6800.018.1543563
GTCTAAG3156.855771E-918.0850011
GTCCAAG3701.4370016E-1017.9628051
CTAGAGT2152.4031504E-517.667213
Produced by FastQC (version 0.11.2)