FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L85_AGGCAGAA-ACTGCATA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L85_AGGCAGAA-ACTGCATA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267486
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG5350.028.4110035
CACTTAG950.001222452125.0146141
GTGTACG2900.024.583331
ATAGGAC2052.6690032E-823.1714733
CCCTTAG1050.00218889822.632271
ACCGTCC3150.022.618888
GTAGGAC1302.9411036E-421.9237773
CTTACAC3251.8189894E-1221.9237773
TATACAG5300.021.5092725
CCTAGAT1353.794412E-421.1126172
ACTGTGC5650.021.0175428
GTACTAG2551.1841621E-820.5021741
GCTACAC3252.1827873E-1120.4621933
CTACACA4900.020.356994
CTCCTAT2601.4804755E-820.1079031
GTGTAGA5950.019.969651
GTCTAAT4350.019.6666621
CTAACAC2902.4374458E-919.6558023
TACACAG4850.019.587485
TAGCACT3707.2759576E-1219.2566134
Produced by FastQC (version 0.11.2)