FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L7_CGAGGCTG-GTAAGGAG_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L7_CGAGGCTG-GTAAGGAG_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270832
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGGT4000.024.932264
GTCCTAG2101.2696546E-924.8737661
GTGTAAG4050.023.4500161
GGGGTGT2853.6379788E-1223.328436
CTAACAC3500.023.0665823
AGGGGTG3650.022.1186395
CTACACT5500.021.5863724
TAGGACT2901.05501385E-1021.2886314
TACACTG6150.020.8492775
ACCGTCC2351.1327393E-720.2117028
GGACTGC1656.3336156E-520.1472826
ATAGGAG3603.6379788E-1219.7875083
CCTAAGG1707.967548E-519.5547142
CCAGGGG1951.0346608E-519.4830863
GTGCTAG2451.7617276E-719.3821561
TATAGGA5400.019.3477882
AGGACTG2953.0249794E-919.3179765
GTATAAG3003.7307473E-918.9945141
TGGTCGC10900.018.74566594-95
GTCCTAT2302.0638963E-618.5815891
Produced by FastQC (version 0.11.2)