FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L7_CGAGGCTG-GTAAGGAG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L7_CGAGGCTG-GTAAGGAG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270832
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAC2002.3646862E-1128.5009573
TAGACAG3900.028.0126575
CCTATAC3050.024.9188693
ACCTTAG1351.3380286E-524.6411191
GTGCTAG2202.1573214E-923.7610821
ACTGTCC3400.023.7498638
GTCTTAG3350.022.6971531
GTGTAGG4800.021.780991
TATACCG2858.367351E-1121.666545
CTAGTAC2951.3278623E-1020.9329073
CTAGACA4100.020.8535394
TAGGACT3252.1827873E-1120.4614184
TCTAGCC2108.6063847E-720.3578243
CTAGCCT2351.1321754E-720.212654
GTATAAG4250.020.1270331
ACCACGT5500.019.8635228
CTAACAC3603.6379788E-1219.7923323
GGTCTAG2451.747776E-719.3968011
AGACAGT4900.019.3876426
TACACTG6450.019.1471775
Produced by FastQC (version 0.11.2)