##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename G140_A6_L73_TAAGGCGA-ACTGCATA_L006_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1230 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.621138211382114 27.0 15.0 33.0 6.0 33.0 2 22.51219512195122 27.0 15.0 33.0 6.0 33.0 3 22.584552845528457 27.0 15.0 33.0 6.0 33.0 4 24.51951219512195 27.0 15.0 37.0 6.0 37.0 5 24.716260162601625 27.0 15.0 37.0 6.0 37.0 6 25.103252032520324 27.0 15.0 37.0 6.0 37.0 7 24.817073170731707 27.0 15.0 37.0 6.0 37.0 8 24.804878048780488 27.0 15.0 37.0 6.0 37.0 9 24.43577235772358 27.0 15.0 37.0 6.0 37.0 10-11 24.268699186991867 27.0 15.0 37.0 6.0 37.0 12-13 24.28170731707317 27.0 15.0 37.0 6.0 37.0 14-15 24.63130081300813 27.0 15.0 37.0 6.0 40.0 16-17 24.65569105691057 27.0 15.0 37.0 6.0 40.0 18-19 24.51178861788618 27.0 15.0 37.0 6.0 40.0 20-21 24.331300813008127 27.0 15.0 37.0 6.0 40.0 22-23 24.226829268292683 27.0 15.0 37.0 6.0 40.0 24-25 24.22439024390244 27.0 15.0 37.0 6.0 40.0 26-27 23.867073170731707 27.0 15.0 37.0 6.0 40.0 28-29 23.641463414634146 27.0 10.5 37.0 6.0 40.0 30-31 23.140650406504065 27.0 6.0 37.0 6.0 40.0 32-33 22.84918699186992 24.5 6.0 37.0 6.0 40.0 34-35 23.07439024390244 24.5 6.0 37.0 6.0 40.0 36-37 23.119918699186993 24.5 6.0 37.0 6.0 40.0 38-39 22.86951219512195 22.0 6.0 37.0 6.0 40.0 40-41 22.770731707317072 22.0 6.0 37.0 6.0 40.0 42-43 22.83170731707317 22.0 6.0 37.0 6.0 40.0 44-45 22.539024390243902 22.0 6.0 37.0 6.0 40.0 46-47 22.44918699186992 22.0 6.0 37.0 6.0 40.0 48-49 22.16869918699187 22.0 6.0 35.0 6.0 40.0 50-51 21.84349593495935 22.0 6.0 35.0 6.0 38.5 52-53 22.176422764227645 22.0 6.0 33.0 6.0 37.0 54-55 22.528048780487804 22.0 6.0 35.0 6.0 38.5 56-57 22.78211382113821 22.0 6.0 35.0 6.0 40.0 58-59 22.73861788617886 22.0 6.0 33.0 6.0 38.5 60-61 22.34959349593496 22.0 6.0 33.0 6.0 37.0 62-63 22.110569105691056 22.0 6.0 33.0 6.0 37.0 64-65 22.054878048780488 22.0 6.0 33.0 6.0 37.0 66-67 21.766666666666666 22.0 6.0 33.0 6.0 37.0 68-69 21.483739837398375 22.0 6.0 33.0 6.0 37.0 70-71 21.38780487804878 22.0 6.0 33.0 6.0 37.0 72-73 21.271138211382116 22.0 6.0 33.0 6.0 37.0 74-75 21.164634146341463 22.0 6.0 33.0 6.0 37.0 76-77 20.847560975609756 22.0 6.0 33.0 6.0 37.0 78-79 20.746747967479674 22.0 6.0 33.0 6.0 37.0 80-81 20.316666666666666 18.5 6.0 33.0 6.0 37.0 82-83 20.170325203252034 22.0 6.0 33.0 6.0 37.0 84-85 20.00569105691057 15.0 6.0 33.0 6.0 37.0 86-87 19.74471544715447 15.0 6.0 33.0 6.0 37.0 88-89 19.560162601626015 15.0 6.0 33.0 6.0 37.0 90-91 19.278455284552848 15.0 6.0 33.0 6.0 37.0 92-93 19.069512195121952 15.0 6.0 30.0 6.0 37.0 94-95 18.851626016260163 15.0 6.0 30.0 6.0 35.0 96-97 18.6869918699187 15.0 6.0 27.0 6.0 33.0 98-99 18.480894308943093 15.0 6.0 27.0 6.0 33.0 100-101 17.80691056910569 15.0 6.0 27.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2307 1 5.417670682730925 2307 2 0.548192771084338 2307 3 6.0682730923694805 2307 4 -0.9226907630522092 2307 5 10.305220883534137 2307 6 3.562248995983939 2307 7 -1.4658634538152597 2307 8 -9.496987951807228 2307 9 -8.674698795180724 2307 10-11 1.2203815261044149 2307 12-13 -3.8594377510040196 2307 14-15 -7.671686746987952 2307 16-17 6.484939759036145 2307 18-19 -8.361445783132531 2307 20-21 -8.319277108433734 2307 22-23 -2.2921686746987966 2307 24-25 -8.538654618473895 2307 26-27 -2.6280120481927725 2307 28-29 3.835843373493976 2307 30-31 11.128012048192772 2307 32-33 -11.946285140562246 2307 34-35 -15.399598393574298 2307 36-37 -15.479919678714857 2307 38-39 -10.32781124497992 2307 40-41 -10.304718875502004 2307 42-43 -10.331325301204817 2307 44-45 -14.353413654618475 2307 46-47 -9.927208835341364 2307 48-49 -10.078815261044177 2307 50-51 -14.288152610441767 2307 52-53 -14.492469879518069 2307 54-55 -9.722891566265059 2307 56-57 -15.061746987951803 2307 58-59 -5.194779116465863 2307 60-61 -5.791164658634539 2307 62-63 -5.379518072289155 2307 64-65 -9.387048192771083 2307 66-67 -9.147590361445783 2307 68-69 1.0833333333333357 2307 70-71 -8.949297188755022 2307 72-73 -8.484437751004016 2307 74-75 -8.119979919678716 2307 76-77 -4.026104417670684 2307 78-79 -3.9528112449799195 2307 80-81 -1.7143574297188735 2307 82-83 -11.72640562248996 2307 84-85 -0.20732931726907466 2307 86-87 -7.932730923694777 2307 88-89 -5.973895582329316 2307 90-91 -3.654618473895585 2307 92-93 -11.813253012048197 2307 94-95 -11.619477911646587 2307 96-97 -2.7530120481927725 2307 98-99 -2.8328313253012034 2307 100-101 2.78614457831325 2304 1 -6.582329317269075 2304 2 -6.451807228915662 2304 3 -5.9317269076305195 2304 4 -16.92269076305221 2304 5 -16.694779116465863 2304 6 -8.43775100401606 2304 7 -17.46586345381526 2304 8 -9.496987951807228 2304 9 -17.674698795180724 2304 10-11 -12.279618473895585 2304 12-13 -11.85943775100402 2304 14-15 -1.6716867469879517 2304 16-17 -17.515060240963855 2304 18-19 -8.361445783132531 2304 20-21 -8.319277108433734 2304 22-23 -9.292168674698797 2304 24-25 -17.538654618473895 2304 26-27 -5.1280120481927725 2304 28-29 -1.1641566265060241 2304 30-31 -15.871987951807228 2304 32-33 -7.446285140562246 2304 34-35 -7.399598393574298 2304 36-37 -15.479919678714857 2304 38-39 -14.82781124497992 2304 40-41 -14.804718875502004 2304 42-43 -10.331325301204817 2304 44-45 -9.853413654618475 2304 46-47 -9.927208835341364 2304 48-49 -2.078815261044177 2304 50-51 4.211847389558233 2304 52-53 -9.992469879518069 2304 54-55 -9.722891566265059 2304 56-57 -10.561746987951803 2304 58-59 -1.6947791164658632 2304 60-61 -1.291164658634539 2304 62-63 -1.8795180722891551 2304 64-65 -13.887048192771083 2304 66-67 -5.647590361445783 2304 68-69 1.0833333333333357 2304 70-71 -5.449297188755022 2304 72-73 5.515562248995984 2304 74-75 -12.619979919678716 2304 76-77 -8.526104417670684 2304 78-79 -12.95281124497992 2304 80-81 -7.7143574297188735 2304 82-83 -11.72640562248996 2304 84-85 -8.207329317269075 2304 86-87 -12.432730923694777 2304 88-89 -10.473895582329316 2304 90-91 -11.654618473895585 2304 92-93 -3.8132530120481967 2304 94-95 4.380522088353413 2304 96-97 5.2469879518072275 2304 98-99 -6.332831325301203 2304 100-101 -5.21385542168675 2202 1 11.417670682730925 2202 2 11.548192771084338 2202 3 12.06827309236948 2202 4 14.07730923694779 2202 5 14.305220883534137 2202 6 13.56224899598394 2202 7 13.53413654618474 2202 8 12.503012048192772 2202 9 13.325301204819276 2202 10-11 14.220381526104415 2202 12-13 14.64056224899598 2202 14-15 17.32831325301205 2202 16-17 16.484939759036145 2202 18-19 16.63855421686747 2202 20-21 16.680722891566266 2202 22-23 15.207831325301203 2202 24-25 14.961345381526105 2202 26-27 16.371987951807228 2202 28-29 16.835843373493976 2202 30-31 16.628012048192772 2202 32-33 16.053714859437754 2202 34-35 15.600401606425702 2202 36-37 18.520080321285143 2202 38-39 19.17218875502008 2202 40-41 17.695281124497996 2202 42-43 19.168674698795183 2202 44-45 19.646586345381525 2202 46-47 19.572791164658636 2202 48-49 17.921184738955823 2202 50-51 18.211847389558233 2202 52-53 18.00753012048193 2202 54-55 19.77710843373494 2202 56-57 18.938253012048197 2202 58-59 19.805220883534137 2202 60-61 20.20883534136546 2202 62-63 19.620481927710845 2202 64-65 20.112951807228917 2202 66-67 18.852409638554217 2202 68-69 18.583333333333336 2202 70-71 17.550702811244978 2202 72-73 18.015562248995984 2202 74-75 13.380020080321284 2202 76-77 13.973895582329316 2202 78-79 14.04718875502008 2202 80-81 16.785642570281126 2202 82-83 12.27359437751004 2202 84-85 8.292670682730925 2202 86-87 14.567269076305223 2202 88-89 13.526104417670684 2202 90-91 3.345381526104415 2202 92-93 3.1867469879518033 2202 94-95 15.380522088353413 2202 96-97 1.7469879518072275 2202 98-99 -2.8328313253012034 2202 100-101 -1.71385542168675 1102 1 -6.582329317269075 1102 2 -6.451807228915662 1102 3 -5.9317269076305195 1102 4 -7.922690763052209 1102 5 -7.694779116465863 1102 6 -8.43775100401606 1102 7 -8.46586345381526 1102 8 -9.496987951807228 1102 9 -8.674698795180724 1102 10-11 -1.7796184738955851 1102 12-13 -11.85943775100402 1102 14-15 -12.171686746987952 1102 16-17 -8.515060240963855 1102 18-19 -8.361445783132531 1102 20-21 -12.819277108433734 1102 22-23 -8.292168674698797 1102 24-25 -13.038654618473895 1102 26-27 -13.128012048192772 1102 28-29 -12.664156626506024 1102 30-31 -15.871987951807228 1102 32-33 -11.946285140562246 1102 34-35 -15.399598393574298 1102 36-37 -10.979919678714857 1102 38-39 -10.32781124497992 1102 40-41 -10.304718875502004 1102 42-43 -5.831325301204817 1102 44-45 -9.853413654618475 1102 46-47 -14.427208835341364 1102 48-49 -14.578815261044177 1102 50-51 -9.788152610441767 1102 52-53 -9.992469879518069 1102 54-55 -14.222891566265059 1102 56-57 -15.061746987951803 1102 58-59 -9.694779116465863 1102 60-61 -9.291164658634539 1102 62-63 -5.379518072289155 1102 64-65 -13.887048192771083 1102 66-67 -13.647590361445783 1102 68-69 -9.416666666666664 1102 70-71 -13.449297188755022 1102 72-73 -8.484437751004016 1102 74-75 -12.619979919678716 1102 76-77 -8.526104417670684 1102 78-79 -12.95281124497992 1102 80-81 -7.7143574297188735 1102 82-83 -11.72640562248996 1102 84-85 -3.7073293172690747 1102 86-87 -7.932730923694777 1102 88-89 -10.473895582329316 1102 90-91 -3.654618473895585 1102 92-93 -11.813253012048197 1102 94-95 -11.619477911646587 1102 96-97 -10.753012048192772 1102 98-99 -6.332831325301203 1102 100-101 -5.21385542168675 2305 1 -15.582329317269075 2305 2 -6.451807228915662 2305 3 -5.9317269076305195 2305 4 -7.922690763052209 2305 5 -0.6947791164658632 2305 6 -17.43775100401606 2305 7 -1.4658634538152597 2305 8 -18.496987951807228 2305 9 -17.674698795180724 2305 10-11 -12.279618473895585 2305 12-13 -16.35943775100402 2305 14-15 -16.67168674698795 2305 16-17 -17.515060240963855 2305 18-19 -8.361445783132531 2305 20-21 6.6807228915662655 2305 22-23 -12.792168674698797 2305 24-25 -13.038654618473895 2305 26-27 -9.628012048192772 2305 28-29 -2.164156626506024 2305 30-31 2.6280120481927725 2305 32-33 -5.946285140562246 2305 34-35 -15.399598393574298 2305 36-37 -15.479919678714857 2305 38-39 -5.8278112449799195 2305 40-41 -2.3047188755020045 2305 42-43 -2.331325301204817 2305 44-45 -9.853413654618475 2305 46-47 -14.427208835341364 2305 48-49 -10.078815261044177 2305 50-51 -1.7881526104417667 2305 52-53 -14.492469879518069 2305 54-55 -5.222891566265059 2305 56-57 -2.5617469879518033 2305 58-59 -3.694779116465863 2305 60-61 -3.291164658634539 2305 62-63 -9.879518072289155 2305 64-65 -9.387048192771083 2305 66-67 -5.647590361445783 2305 68-69 -4.916666666666664 2305 70-71 7.550702811244978 2305 72-73 5.515562248995984 2305 74-75 -4.619979919678716 2305 76-77 -8.526104417670684 2305 78-79 5.5471887550200805 2305 80-81 -12.214357429718874 2305 82-83 -7.22640562248996 2305 84-85 -8.207329317269075 2305 86-87 -12.432730923694777 2305 88-89 -1.473895582329316 2305 90-91 0.8453815261044149 2305 92-93 -7.313253012048197 2305 94-95 -7.119477911646587 2305 96-97 -1.7530120481927725 2305 98-99 -10.832831325301203 2305 100-101 -9.71385542168675 2203 1 5.417670682730925 2203 2 5.548192771084338 2203 3 6.0682730923694805 2203 4 10.07730923694779 2203 5 4.305220883534137 2203 6 9.56224899598394 2203 7 9.53413654618474 2203 8 8.503012048192772 2203 9 -8.674698795180724 2203 10-11 -1.7796184738955851 2203 12-13 4.64056224899598 2203 14-15 -1.6716867469879517 2203 16-17 6.484939759036145 2203 18-19 9.638554216867469 2203 20-21 9.680722891566266 2203 22-23 0.7078313253012034 2203 24-25 0.9613453815261046 2203 26-27 6.3719879518072275 2203 28-29 9.835843373493976 2203 30-31 11.128012048192772 2203 32-33 10.553714859437754 2203 34-35 11.600401606425702 2203 36-37 -1.9799196787148574 2203 38-39 9.17218875502008 2203 40-41 9.195281124497996 2203 42-43 -2.331325301204817 2203 44-45 0.6465863453815253 2203 46-47 -1.9272088353413643 2203 48-49 12.421184738955823 2203 50-51 9.711847389558233 2203 52-53 9.507530120481931 2203 54-55 9.777108433734941 2203 56-57 11.938253012048197 2203 58-59 12.805220883534137 2203 60-61 1.208835341365461 2203 62-63 4.120481927710845 2203 64-65 7.112951807228917 2203 66-67 10.352409638554217 2203 68-69 7.083333333333336 2203 70-71 10.550702811244978 2203 72-73 5.015562248995984 2203 74-75 -0.11997991967871613 2203 76-77 7.973895582329316 2203 78-79 11.04718875502008 2203 80-81 8.785642570281126 2203 82-83 -3.7264056224899598 2203 84-85 -0.20732931726907466 2203 86-87 11.567269076305223 2203 88-89 0.026104417670683944 2203 90-91 3.345381526104415 2203 92-93 0.6867469879518033 2203 94-95 9.380522088353413 2203 96-97 4.2469879518072275 2203 98-99 7.667168674698797 2203 100-101 2.78614457831325 2310 1 11.417670682730925 2310 2 11.548192771084338 2310 3 12.06827309236948 2310 4 14.07730923694779 2310 5 14.305220883534137 2310 6 13.56224899598394 2310 7 13.53413654618474 2310 8 12.503012048192772 2310 9 13.325301204819276 2310 10-11 14.220381526104415 2310 12-13 14.64056224899598 2310 14-15 17.32831325301205 2310 16-17 16.484939759036145 2310 18-19 16.63855421686747 2310 20-21 16.680722891566266 2310 22-23 15.207831325301203 2310 24-25 14.961345381526105 2310 26-27 16.371987951807228 2310 28-29 16.835843373493976 2310 30-31 18.128012048192772 2310 32-33 17.553714859437754 2310 34-35 18.600401606425702 2310 36-37 18.520080321285143 2310 38-39 19.17218875502008 2310 40-41 19.195281124497996 2310 42-43 19.168674698795183 2310 44-45 19.646586345381525 2310 46-47 19.572791164658636 2310 48-49 16.421184738955823 2310 50-51 19.711847389558233 2310 52-53 18.00753012048193 2310 54-55 19.77710843373494 2310 56-57 18.938253012048197 2310 58-59 19.805220883534137 2310 60-61 18.70883534136546 2310 62-63 18.120481927710845 2310 64-65 20.112951807228917 2310 66-67 20.352409638554217 2310 68-69 20.083333333333336 2310 70-71 20.550702811244978 2310 72-73 21.015562248995984 2310 74-75 21.380020080321284 2310 76-77 20.973895582329316 2310 78-79 21.04718875502008 2310 80-81 21.785642570281126 2310 82-83 19.27359437751004 2310 84-85 18.292670682730925 2310 86-87 18.567269076305223 2310 88-89 20.526104417670684 2310 90-91 19.345381526104415 2310 92-93 15.186746987951803 2310 94-95 19.380522088353413 2310 96-97 18.246987951807228 2310 98-99 18.167168674698797 2310 100-101 -0.7138554216867501 2311 1 -0.5823293172690747 2311 2 -0.45180722891566205 2311 3 -5.9317269076305195 2311 4 1.0773092369477908 2311 5 -7.694779116465863 2311 6 -8.43775100401606 2311 7 0.5341365461847403 2311 8 -0.49698795180722755 2311 9 -5.174698795180724 2311 10-11 -4.779618473895585 2311 12-13 -7.35943775100402 2311 14-15 -6.921686746987952 2311 16-17 -2.265060240963855 2311 18-19 -3.861445783132531 2311 20-21 2.1807228915662655 2311 22-23 -10.542168674698797 2311 24-25 -10.788654618473895 2311 26-27 -4.8780120481927725 2311 28-29 -11.914156626506024 2311 30-31 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5.014056224899598 1213 22-23 -1.7921686746987966 1213 24-25 -5.205321285140563 1213 26-27 -0.2946787148594403 1213 28-29 3.669176706827308 1213 30-31 3.1280120481927725 1213 32-33 3.3870481927710863 1213 34-35 3.4337349397590344 1213 36-37 4.353413654618475 1213 38-39 3.1721887550200805 1213 40-41 7.028614457831331 1213 42-43 4.002008032128515 1213 44-45 4.979919678714857 1213 46-47 5.406124497991968 1213 48-49 3.754518072289155 1213 50-51 6.5451807228915655 1213 52-53 3.8408634538152633 1213 54-55 4.110441767068274 1213 56-57 3.271586345381529 1213 58-59 5.971887550200801 1213 60-61 6.375502008032125 1213 62-63 3.953815261044177 1213 64-65 4.446285140562249 1213 66-67 5.0190763052208816 1213 68-69 6.083333333333336 1213 70-71 3.550702811244978 1213 72-73 1.015562248995984 1213 74-75 4.046686746987948 1213 76-77 7.14056224899598 1213 78-79 6.880522088353413 1213 80-81 7.118975903614459 1213 82-83 7.106927710843376 1213 84-85 8.126004016064257 1213 86-87 4.400602409638555 1213 88-89 -1.8072289156626482 1213 90-91 -0.15461847389558514 1213 92-93 4.186746987951803 1213 94-95 8.047188755020077 1213 96-97 5.08032128514056 1213 98-99 6.500502008032129 1213 100-101 -1.0471887550200822 >>END_MODULE >>Per sequence quality scores fail #Quality Count 7 20.0 8 40.0 9 68.0 10 82.0 11 57.0 12 72.0 13 57.0 14 49.0 15 35.0 16 25.0 17 45.0 18 39.0 19 18.0 20 27.0 21 30.0 22 24.0 23 27.0 24 21.0 25 27.0 26 23.0 27 16.0 28 18.0 29 26.0 30 23.0 31 32.0 32 30.0 33 48.0 34 32.0 35 55.0 36 64.0 37 66.0 38 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.699186991869915 30.48780487804878 9.024390243902438 21.788617886178862 2 17.317073170731707 27.64227642276423 35.203252032520325 19.83739837398374 3 20.40650406504065 32.19512195121951 24.552845528455283 22.84552845528455 4 15.853658536585366 23.089430894308943 33.170731707317074 27.88617886178862 5 14.959349593495935 39.43089430894309 31.05691056910569 14.552845528455286 6 27.88617886178862 45.6910569105691 13.495934959349592 12.926829268292684 7 24.796747967479675 36.58536585365854 20.8130081300813 17.804878048780488 8 23.008130081300813 44.22764227642276 17.317073170731707 15.447154471544716 9 24.390243902439025 23.902439024390244 19.1869918699187 32.52032520325203 10-11 24.59349593495935 32.5609756097561 25.16260162601626 17.682926829268293 12-13 23.414634146341466 29.024390243902438 25.284552845528456 22.276422764227643 14-15 22.682926829268293 30.365853658536583 21.178861788617887 25.772357723577237 16-17 23.089430894308943 36.666666666666664 21.341463414634145 18.902439024390244 18-19 19.878048780487806 34.71544715447155 25.16260162601626 20.243902439024392 20-21 22.317073170731707 33.00813008130081 23.86178861788618 20.8130081300813 22-23 22.96747967479675 32.35772357723577 24.878048780487806 19.796747967479675 24-25 22.073170731707318 34.47154471544715 24.837398373983742 18.617886178861788 26-27 21.788617886178862 36.016260162601625 23.29268292682927 18.902439024390244 28-29 22.35772357723577 34.552845528455286 22.96747967479675 20.121951219512198 30-31 20.56910569105691 34.83739837398374 25.121951219512194 19.471544715447152 32-33 22.195121951219512 32.84552845528455 25.772357723577237 19.1869918699187 34-35 22.439024390243905 33.45528455284553 24.024390243902438 20.08130081300813 36-37 21.341463414634145 34.146341463414636 24.1869918699187 20.32520325203252 38-39 21.991869918699187 35.731707317073166 22.926829268292686 19.349593495934958 40-41 22.032520325203254 33.577235772357724 23.86178861788618 20.528455284552845 42-43 21.341463414634145 34.71544715447155 24.796747967479675 19.146341463414636 44-45 20.89430894308943 35.44715447154472 24.796747967479675 18.86178861788618 46-47 22.5609756097561 32.926829268292686 25.406504065040654 19.10569105691057 48-49 20.934959349593495 32.39837398373984 25.32520325203252 21.341463414634145 50-51 21.6260162601626 34.47154471544715 24.59349593495935 19.308943089430894 52-53 20.528455284552845 32.19512195121951 25.8130081300813 21.463414634146343 54-55 19.552845528455283 33.61788617886179 24.34959349593496 22.479674796747968 56-57 20.203252032520325 32.073170731707314 25.650406504065042 22.073170731707318 58-59 20.040650406504064 32.96747967479675 25.8130081300813 21.178861788617887 60-61 20.73170731707317 33.08943089430894 24.390243902439025 21.788617886178862 62-63 20.365853658536583 31.300813008130078 25.406504065040654 22.926829268292686 64-65 20.650406504065042 31.382113821138212 24.59349593495935 23.3739837398374 66-67 22.398373983739837 31.666666666666664 24.390243902439025 21.544715447154474 68-69 21.6260162601626 32.31707317073171 23.65853658536585 22.398373983739837 70-71 20.650406504065042 32.60162601626016 25.365853658536587 21.38211382113821 72-73 20.853658536585364 31.869918699186993 25.24390243902439 22.032520325203254 74-75 20.934959349593495 31.99186991869919 23.333333333333332 23.739837398373982 76-77 21.341463414634145 32.84552845528455 23.78048780487805 22.032520325203254 78-79 21.991869918699187 31.829268292682926 25.24390243902439 20.934959349593495 80-81 21.178861788617887 32.35772357723577 26.585365853658537 19.878048780487806 82-83 20.772357723577237 31.869918699186993 25.650406504065042 21.70731707317073 84-85 22.642276422764226 31.097560975609756 25.32520325203252 20.934959349593495 86-87 22.5609756097561 32.23577235772358 23.170731707317074 22.032520325203254 88-89 23.2520325203252 31.341463414634145 23.821138211382113 21.585365853658537 90-91 22.11382113821138 32.4390243902439 23.577235772357724 21.86991869918699 92-93 22.276422764227643 32.72357723577235 23.65853658536585 21.341463414634145 94-95 22.76422764227642 33.08943089430894 22.642276422764226 21.504065040650406 96-97 22.804878048780488 32.23577235772358 23.130081300813007 21.829268292682926 98-99 23.2520325203252 31.626016260162604 23.008130081300813 22.11382113821138 100-101 21.666666666666668 31.7479674796748 24.674796747967477 21.910569105691057 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.5 19 2.0 20 2.0 21 3.0 22 6.5 23 6.0 24 7.5 25 15.5 26 15.5 27 12.0 28 20.0 29 25.0 30 22.5 31 27.5 32 29.0 33 36.5 34 44.5 35 47.5 36 53.5 37 52.5 38 47.5 39 42.5 40 40.5 41 38.0 42 42.5 43 38.5 44 37.5 45 41.5 46 32.5 47 33.5 48 36.0 49 29.5 50 28.0 51 30.0 52 30.0 53 24.0 54 20.0 55 18.5 56 18.0 57 17.5 58 15.0 59 14.5 60 13.0 61 14.0 62 13.0 63 11.0 64 9.5 65 10.5 66 8.5 67 8.0 68 9.0 69 6.5 70 6.5 71 5.5 72 3.5 73 1.0 74 1.0 75 1.5 76 0.5 77 1.0 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1230.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-11 0.0 0.0 0.0 12-13 0.0 0.0 0.0 14-15 0.0 0.0 0.0 16-17 0.0 0.0 0.0 18-19 0.0 0.0 0.0 20-21 0.0 0.0 0.0 22-23 0.0 0.0 0.0 24-25 0.0 0.0 0.0 26-27 0.0 0.0 0.0 28-29 0.0 0.0 0.04065040650406504 30-31 0.0 0.0 0.24390243902439024 32-33 0.0 0.0 0.24390243902439024 34-35 0.0 0.0 0.2845528455284553 36-37 0.0 0.0 0.4878048780487805 38-39 0.0 0.0 0.8943089430894309 40-41 0.0 0.0 1.178861788617886 42-43 0.0 0.0 1.5447154471544715 44-45 0.0 0.0 2.1138211382113825 46-47 0.0 0.0 2.4390243902439024 48-49 0.0 0.0 3.048780487804878 50-51 0.0 0.0 3.2926829268292686 52-53 0.0 0.0 3.7804878048780486 54-55 0.0 0.0 4.390243902439025 56-57 0.0 0.0 5.121951219512194 58-59 0.0 0.0 5.528455284552845 60-61 0.0 0.0 6.016260162601625 62-63 0.0 0.0 6.260162601626016 64-65 0.0 0.0 7.073170731707317 66-67 0.0 0.0 7.886178861788618 68-69 0.0 0.0 8.048780487804878 70-71 0.0 0.0 8.658536585365853 72-73 0.0 0.0 9.065040650406504 74-75 0.0 0.0 9.634146341463413 76-77 0.0 0.0 10.0 78-79 0.0 0.0 10.365853658536587 80-81 0.0 0.0 10.56910569105691 82-83 0.0 0.0 10.731707317073171 84-85 0.0 0.0 11.300813008130081 86-87 0.0 0.0 11.747967479674797 88-89 0.0 0.0 11.991869918699187 >>END_MODULE >>Kmer Content pass >>END_MODULE