FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L70_AAGAGGCA-GTAAGGAG_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L70_AAGAGGCA-GTAAGGAG_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13289981
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG56750.020.5846525
TACACTG58000.018.4216635
GTATTAG36000.017.9380971
ACACCGT51000.017.4120796
CTATACT34250.016.915064
TATACAG48600.016.9037915
GTATAAG36000.016.7510191
TATACCG35000.016.6882725
CTACACT56850.016.3719314
TCTATAC38200.016.1604823
TCTACAC63800.016.0026573
ACTGTGC46200.015.9340488
GTGTATA48300.015.9260421
TACACAG41200.015.5600475
GTATATA71050.015.4378881
CTACACC76750.015.1587014
GTGTTAG34400.015.0455491
GTATAGA42200.014.8525651
GTGTACA59650.014.5673531
ACCGTGC55150.014.5538418
Produced by FastQC (version 0.11.2)