FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L70_AAGAGGCA-GTAAGGAG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L70_AAGAGGCA-GTAAGGAG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13289981
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG56400.019.6230495
CTATACT39500.019.601094
TATACCG31450.019.1810675
TATACAG49750.018.9998285
TACACCG53550.018.6272815
GTGTAGG48100.017.3922731
TTATACT65500.016.969314
GTATAGA41600.016.2249661
GTCTAGA34500.015.5685491
TACACAG44750.015.49706655
GTGTAGA69550.015.377091
CTACACT53300.015.3281924
GTACACT48750.015.2972984
TCTATAC40650.015.0746863
CTGTCCC34100.015.0438519
ATAGGAC22850.014.9680543
TATACTC42700.014.9061885
GTGTATA43500.014.7514021
CCTACAC48700.014.5337073
CGTACAC34800.014.4692393
Produced by FastQC (version 0.11.2)