FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L6_GGACTCCT-GTAAGGAG_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L6_GGACTCCT-GTAAGGAG_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010524
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA3200.026.7074321
TGGACAG3200.023.7411165
ACAGTCC2303.6325218E-922.713398
GTCCTAC1903.299574E-722.490471
GTATAGG3007.2759576E-1222.1572781
ACTGTCC2257.1628165E-821.1073918
TACACTC3650.020.8141295
TCTACAC5600.020.3495273
AGTGTCC3052.0918378E-1020.2423368
TCTAGAC3052.0918378E-1020.2383293
GTCCAAG2351.1365955E-720.2042051
CTGGACA3553.6379788E-1220.0629164
CGTACAC2401.4183388E-719.7842643
CTGTCCG1951.0344564E-519.4827829
CTATACT3203.9835868E-1019.2896584
CTACACT4300.018.7720454
GTTCTAG3306.002665E-1018.7041971
CTAGTAC1801.2366739E-418.4653133
TTATACT5150.018.4397014
TAGGCCG1559.6476317E-418.3802195
Produced by FastQC (version 0.11.2)