FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L6_GGACTCCT-GTAAGGAG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L6_GGACTCCT-GTAAGGAG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010524
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG3250.029.2305855
TACACTG5250.026.2379325
ACACCGT3000.025.3331746
GTCCTAC1551.4043635E-624.5281141
GTATAAG3650.023.4361061
GTACAGG2704.0017767E-1122.8815481
GTCTTAG2303.605237E-922.7284971
CTTACAC3401.8189894E-1220.9567873
CTGTCCG1856.5511667E-620.5404139
CCTACAC4400.020.5122493
ATAGAAC4300.019.884583
TAGGGAG1456.1455916E-419.655055
GCCTTAG1951.0280975E-519.4967041
CGTACAC1951.0320591E-519.4880223
GGCGCAC2451.7555976E-719.3876328
GTGCTAG1507.6948415E-419.0092871
GTTAGGG1250.006029144419.0008223
AGGACCC1300.007562784518.2691155
GTATATA6800.018.170641
TAGACAG2905.2114046E-818.0171285
Produced by FastQC (version 0.11.2)