FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L68_GCTACGCT-GTAAGGAG_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L68_GCTACGCT-GTAAGGAG_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831386
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCA702.0604357E-433.907262
TAGACCG753.0890832E-431.6467765
CCTAGTA804.4996213E-429.6795649
GTATAGA2400.027.6892641
CTAGCAC1257.423825E-626.583293
GTACTAG2002.0672815E-823.7336521
GTACACT2755.2750693E-1122.4404414
TACACGG1503.0466528E-522.1527445
TACTAGG1100.0028872121.5773472
GCTAGAC1353.81086E-421.097853
CTCCTAG1353.8124082E-421.096581
ACCGACC2056.8421105E-720.8480838
GCTACAC2309.072937E-820.63923
CTATCCT1856.5841996E-620.527644
AGTGTGC2551.1943484E-820.4847168
GAGTGTG2801.5807018E-920.3504777
GGAGTGT1656.350646E-520.1388576
ACCGTGC2601.4932084E-820.0907828
TCCTAGA1908.291634E-619.9874382
TATAGGA3102.6193447E-1019.9068432
Produced by FastQC (version 0.11.2)