FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L68_GCTACGCT-GTAAGGAG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L68_GCTACGCT-GTAAGGAG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831386
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC2253.6379788E-1227.4441668
TCTACAC4750.027.001353
CTATACT2305.456968E-1226.8475534
GCTATAC1457.8797166E-726.2082063
TAGGGCG1457.883864E-726.206634
GTGTTAG2750.025.9306621
TACACCG2600.025.5766625
CCGTGCG2059.567884E-1025.4875479
TAGGACC1351.34225265E-524.629384
ATAGGGC1951.567605E-824.3601913
TATAGGG2252.8085196E-923.2233832
TACACTG3400.022.3527155
TAGGGCA1503.032148E-522.1664419
TAAGACC1954.2189458E-721.9228554
GTATAGG2405.864422E-921.788961
TATACTA2657.930794E-1021.5092145
GGACCTT1553.9050537E-521.4513956
GTATAAG2005.3394797E-721.3927961
CTACACG1805.1663646E-621.1108994
AGGACCG1353.7949864E-421.1108955
Produced by FastQC (version 0.11.2)