FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L67_CAGAGAGG-GTAAGGAG_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L67_CAGAGAGG-GTAAGGAG_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601447
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTAG804.505353E-429.6714651
CTCTACG1056.925874E-527.1281971
ACTCCGC1201.7128672E-423.7430938
GTGTTAG1801.9579966E-723.7371711
GTATAGA1000.001657937323.7371711
TAGGGAG1201.7157682E-423.737175
CCTCTAC1201.7157682E-423.737171
TATCCCG1201.7157682E-423.737175
ACAGTCC1452.337194E-522.924378
TAGACTC1050.002201914622.606835
CTCTACT2103.4589902E-822.606832
TATACTC1903.2986645E-722.4878485
ACCATGT2606.2573235E-1021.9167028
CTACACT1954.236499E-721.9112364
ACAGTGC3059.094947E-1221.7969428
TTAGAAC1754.06292E-621.7025573
TACACAG1754.06292E-621.7025575
ATACCGT2858.367351E-1121.6549636
TTGTACC2509.5260475E-920.888714
GTACTAT2056.85357E-720.8423961
Produced by FastQC (version 0.11.2)