FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L67_CAGAGAGG-GTAAGGAG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L67_CAGAGAGG-GTAAGGAG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601447
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAG905.6663157E-736.965731
CTATACA2250.029.5553744
GCTATAC1354.2196916E-728.1479763
TAAGGTG1903.947207E-1027.4998325
GTGTATA2450.027.1584971
TATACAG2550.026.0782725
TAGATAG1151.2808133E-424.7824575
GTGTAGG1951.557055E-824.373011
TACACCG2402.2919266E-1023.7498555
GCTATAG1000.001652791523.7498533
TATAACG1000.001652791523.7498532
AGGGTTC1050.002195091222.6189085
ATAAGGC1954.2147803E-721.9229433
ACACAGT2657.912604E-1021.5093026
GTATAAG1805.1362967E-621.1232741
AGTAGGG1353.793198E-421.1109832
CCTATAC1805.1622665E-621.1109813
GAGTGTC1604.9844486E-520.7811227
AGTGTCC1604.9844486E-520.7811228
CTGTGCG2551.1890734E-820.490079
Produced by FastQC (version 0.11.2)