FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L66_CTCTCTAC-GTAAGGAG_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L66_CTCTCTAC-GTAAGGAG_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454808
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG1951.580338E-824.3446041
TATACCG3700.023.0960665
CGTACAC3900.021.9116523
AGGAGTG3950.021.6342895
TAGACAG3401.8189894E-1220.9449625
CTAGTAC2056.866103E-720.842793
CCTGTAC4800.020.7704183
GTCCTAA1856.5876065E-620.5284251
CTTACAC3850.019.729973
TAGGGTG1507.749523E-418.9900995
GGTCTAG1250.006052487518.988791
GTTATAC3609.640644E-1118.4625973
GTGTAAT5400.018.4613271
CATACTG3357.366907E-1018.423235
TCTAGAC3156.853952E-918.0858083
GTAAGAC2905.246875E-818.007853
GTACACT6100.017.9005014
TAGGAGT4002.3646862E-1117.8032174
GTGCATA4303.6379788E-1217.6639921
GTTCTAG4303.6379788E-1217.6639921
Produced by FastQC (version 0.11.2)