FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L66_CTCTCTAC-GTAAGGAG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L66_CTCTCTAC-GTAAGGAG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454808
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG6950.022.5536865
GTGCTAG1302.925893E-421.9409121
GCTACAC3900.021.9250763
CTACACA5300.021.5091764
CTAGCAC2005.383408E-721.376953
GTGTAAG4250.021.252571
CTATACA4400.020.511124
GTGTAGA7250.020.3268681
CTATACT3750.020.2664244
TACACTG6650.019.999765
CTATCCT3102.6011548E-1019.9191174
TCTACAC8200.019.6969183
ACACTGC4900.019.3875246
TCCTATA3455.2750693E-1119.2791062
AGGGGTG3455.2750693E-1119.2751315
GTAGCAC4450.019.2152353
GTGTAGG5050.018.8271871
GTAAGGG3305.984475E-1018.7138273
GTATACG2302.0426742E-618.602081
CTTAGAC3851.2732926E-1118.508183
Produced by FastQC (version 0.11.2)