##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename G140_A6_L59_TCCTGAGC-GTAAGGAG_L006_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 567128 Sequences flagged as poor quality 0 Sequence length 101 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.289957469918605 33.0 33.0 33.0 33.0 33.0 2 32.29520143600739 33.0 33.0 33.0 33.0 33.0 3 32.28576441297203 33.0 33.0 33.0 33.0 33.0 4 36.17178485280219 37.0 37.0 37.0 37.0 37.0 5 36.0787670508245 37.0 37.0 37.0 33.0 37.0 6 36.10554231143587 37.0 37.0 37.0 37.0 37.0 7 36.05746145490965 37.0 37.0 37.0 37.0 37.0 8 36.06784182759448 37.0 37.0 37.0 37.0 37.0 9 36.11087796758404 37.0 37.0 37.0 37.0 37.0 10-11 36.05907749220634 37.0 37.0 37.0 35.0 37.0 12-13 36.00711479595435 37.0 37.0 37.0 33.0 37.0 14-15 38.038900389330095 40.0 37.0 40.0 35.0 40.0 16-17 37.92253512434583 40.0 37.0 40.0 33.0 40.0 18-19 37.80361488059133 40.0 37.0 40.0 33.0 40.0 20-21 37.68562564359368 40.0 37.0 40.0 33.0 40.0 22-23 37.54284129861337 40.0 37.0 40.0 33.0 40.0 24-25 37.360798620417256 40.0 37.0 40.0 33.0 40.0 26-27 37.115201506538206 40.0 37.0 40.0 33.0 40.0 28-29 36.905104315075256 40.0 37.0 40.0 33.0 40.0 30-31 36.65506464149187 40.0 37.0 40.0 33.0 40.0 32-33 36.34877928792089 37.0 37.0 40.0 33.0 40.0 34-35 36.283114217601664 37.0 37.0 40.0 30.0 40.0 36-37 36.4457926605634 37.0 37.0 40.0 33.0 40.0 38-39 36.242194002059506 37.0 37.0 40.0 33.0 40.0 40-41 35.95071571144433 37.0 37.0 40.0 27.0 40.0 42-43 35.638051727299654 37.0 37.0 40.0 27.0 40.0 44-45 35.29256358352964 37.0 33.0 40.0 27.0 40.0 46-47 35.013546324639236 37.0 33.0 40.0 27.0 40.0 48-49 34.67814585067216 37.0 33.0 38.5 27.0 40.0 50-51 33.392049942870045 35.0 33.0 37.0 24.5 38.5 52-53 33.55459614055381 37.0 33.0 37.0 24.5 38.5 54-55 34.34273656035322 37.0 33.0 37.0 27.0 40.0 56-57 34.27249139524058 37.0 33.0 37.0 27.0 40.0 58-59 34.07256562892327 37.0 33.0 37.0 27.0 40.0 60-61 33.880614252867076 37.0 33.0 37.0 27.0 38.5 62-63 33.67986063110973 37.0 33.0 37.0 27.0 37.0 64-65 33.47981231750151 37.0 33.0 37.0 27.0 37.0 66-67 33.247687470906044 37.0 33.0 37.0 22.0 37.0 68-69 33.06513520757218 37.0 33.0 37.0 22.0 37.0 70-71 32.87310272107884 37.0 33.0 37.0 22.0 37.0 72-73 32.668811097318425 37.0 33.0 37.0 22.0 37.0 74-75 32.49097470059669 37.0 33.0 37.0 22.0 37.0 76-77 32.29251244868883 37.0 33.0 37.0 22.0 37.0 78-79 32.08309235304905 37.0 33.0 37.0 22.0 37.0 80-81 31.858411152332454 33.0 33.0 37.0 22.0 37.0 82-83 31.64693684670833 33.0 33.0 37.0 18.5 37.0 84-85 31.423255420293124 33.0 33.0 37.0 15.0 37.0 86-87 31.200341016490107 33.0 30.0 37.0 15.0 37.0 88-89 30.96962149638177 33.0 27.0 37.0 15.0 37.0 90-91 30.72723529785163 33.0 27.0 37.0 15.0 37.0 92-93 30.470647719738754 33.0 27.0 37.0 15.0 37.0 94-95 30.207407322509205 33.0 27.0 37.0 15.0 37.0 96-97 29.922547467238438 33.0 27.0 37.0 15.0 37.0 98-99 29.603562158807183 33.0 27.0 37.0 15.0 37.0 100-101 28.577499259426443 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.18320146581068286 2306 2 0.3355125774906398 2306 3 0.15703628940679693 2306 4 0.2878226546637137 2306 5 0.20997146653424892 2306 6 0.3622167187091847 2306 7 0.08040841191123604 2306 8 0.22166257202398754 2306 9 0.3050987952863693 2306 10-11 0.17921694453465165 2306 12-13 0.23241770742275492 2306 14-15 0.28018726321454324 2306 16-17 0.3172785325523577 2306 18-19 0.2180102542145761 2306 20-21 0.17961641410467877 2306 22-23 0.13476396357804532 2306 24-25 0.35835433851976717 2306 26-27 -0.10633922569696352 2306 28-29 -0.12552990311483825 2306 30-31 0.18053134553966999 2306 32-33 -0.04202551086613937 2306 34-35 -0.15272276015249275 2306 36-37 -0.14972057995844779 2306 38-39 -0.07142893494973634 2306 40-41 -0.44071760817024597 2306 42-43 -0.5018583607356604 2306 44-45 -0.5121931672077764 2306 46-47 -0.38861772578591314 2306 48-49 -0.580037357942139 2306 50-51 -0.4643323912809194 2306 52-53 -0.7885631716828101 2306 54-55 -0.7815462398874544 2306 56-57 -1.0063134458600018 2306 58-59 -1.1318002148368933 2306 60-61 -1.3263088540969292 2306 62-63 -1.2421520470655665 2306 64-65 -1.2798315596370387 2306 66-67 -1.1062005889362254 2306 68-69 -1.1807156129702108 2306 70-71 -1.4802576435280699 2306 72-73 -1.407160439146061 2306 74-75 -1.3443555701670569 2306 76-77 -1.7201389829100364 2306 78-79 -1.9131564584457834 2306 80-81 -1.9095869459404433 2306 82-83 -2.1339668972671397 2306 84-85 -2.0974871640182364 2306 86-87 -2.442295437917224 2306 88-89 -2.2493517363626374 2306 90-91 -2.4529328684285296 2306 92-93 -2.547177237709292 2306 94-95 -2.5418767970266423 2306 96-97 -3.2856060928170088 2306 98-99 -2.749597861124041 2306 100-101 -2.8197625152474863 2307 1 0.2736638576639763 2307 2 0.24642038889106033 2307 3 0.2820186961626021 2307 4 0.1753335210792457 2307 5 0.29330135021185555 2307 6 0.23154651959505657 2307 7 0.10981603703024945 2307 8 0.3881815934256494 2307 9 0.17674262523214423 2307 10-11 0.37232867198425623 2307 12-13 0.3424955316558993 2307 14-15 0.39995379051512714 2307 16-17 0.3867697773261298 2307 18-19 0.40047910382337903 2307 20-21 0.2616775144068697 2307 22-23 0.43698485193338144 2307 24-25 0.4066281307953048 2307 26-27 0.42095100073844804 2307 28-29 0.26527607444860024 2307 30-31 0.22615580221889076 2307 32-33 0.11330627702142948 2307 34-35 0.08378632624067706 2307 36-37 0.25816533302742783 2307 38-39 0.11518571090860519 2307 40-41 0.3206910932413578 2307 42-43 0.16348915758201343 2307 44-45 0.4128844500460218 2307 46-47 -0.12275896848989731 2307 48-49 -0.09908773671433835 2307 50-51 -0.00363776860427123 2307 52-53 -0.07117897662547534 2307 54-55 -0.3609580045933356 2307 56-57 -0.33937218659767865 2307 58-59 -0.03815241088772581 2307 60-61 -0.14914695485447282 2307 62-63 -0.01357854870070696 2307 64-65 -0.3124515041665745 2307 66-67 -0.749206756920664 2307 68-69 -0.3282111629143216 2307 70-71 -0.5435188100636523 2307 72-73 -0.39144035801015775 2307 74-75 -0.321786542556012 2307 76-77 -0.4232705803766095 2307 78-79 -0.5612274109647224 2307 80-81 -0.38270239901822833 2307 82-83 -0.6285916437174457 2307 84-85 -0.816202236254032 2307 86-87 -0.7215830150170284 2307 88-89 -0.7736159247966334 2307 90-91 -0.5191455604018707 2307 92-93 -0.7875009534024642 2307 94-95 -0.9978978261279359 2307 96-97 -0.6204531350821902 2307 98-99 -0.8974369967782998 2307 100-101 -0.4213293488774923 2201 1 0.2839018643132789 2201 2 0.2883846138791597 2201 3 0.2934449357055513 2201 4 0.30703933541885675 2201 5 0.2805748558189549 2201 6 0.30097612779826477 2201 7 0.4396886920706393 2201 8 0.5902331955396676 2201 9 0.41111256173969934 2201 10-11 0.24132699565569737 2201 12-13 0.37808650748716843 2201 14-15 0.7113268196288303 2201 16-17 0.5811878832749002 2201 18-19 0.7116140862799369 2201 20-21 0.4657795183996498 2201 22-23 0.5995327113138202 2201 24-25 0.5457854056211673 2201 26-27 0.7245084980079071 2201 28-29 0.6580630606979057 2201 30-31 0.7215428980896803 2201 32-33 0.6883069771586534 2201 34-35 0.6287911471855878 2201 36-37 0.7394916729900629 2201 38-39 0.7799960137279598 2201 40-41 0.6111340200550117 2201 42-43 0.5854378595275946 2201 44-45 0.70021676718018 2201 46-47 0.8235205022863781 2201 48-49 0.7045377738857397 2201 50-51 0.3023221995494936 2201 52-53 0.6084762357961608 2201 54-55 0.5708206240178768 2201 56-57 0.8497908893652166 2201 58-59 0.6659395911446708 2201 60-61 0.5634307103679106 2201 62-63 0.33587194359579087 2201 64-65 0.2619910865812116 2201 66-67 0.33887850779122886 2201 68-69 0.28794960459610763 2201 70-71 0.07550051772459909 2201 72-73 0.22449636558766173 2201 74-75 0.14752544259307143 2201 76-77 0.3147420701028878 2201 78-79 0.10000419042915354 2201 80-81 -0.16808833466161488 2201 82-83 -0.07016019115274474 2201 84-85 -0.09587705251801637 2201 86-87 -0.1028537440799262 2201 88-89 -0.5109284380492269 2201 90-91 -0.8674158213730188 2201 92-93 -0.6636486783565516 2201 94-95 -0.8166382992606707 2201 96-97 -0.6829453835628918 2201 98-99 -0.7959751102485413 2201 100-101 -0.6230048772810548 1101 1 0.31050079854568224 1101 2 0.24345342113210933 1101 3 0.22353646500285151 1101 4 0.2858559788920658 1101 5 0.29581842482043186 1101 6 0.33425402311469554 1101 7 0.3968090830783666 1101 8 0.33835980541064714 1101 9 0.37378806831871714 1101 10-11 0.36324160602449496 1101 12-13 0.16062428642146642 1101 14-15 0.22855031780540713 1101 16-17 0.3160376537916747 1101 18-19 0.20946067759615516 1101 20-21 0.2341428900870426 1101 22-23 0.08589948470810072 1101 24-25 0.21359100297522815 1101 26-27 -0.05663578791649826 1101 28-29 0.0930949846988014 1101 30-31 -0.29954240480177674 1101 32-33 -0.11972286131683774 1101 34-35 -0.04739049340258106 1101 36-37 0.21350180268485275 1101 38-39 0.07199596847243583 1101 40-41 0.08627930914352788 1101 42-43 -0.056964303686449114 1101 44-45 -0.08065221430653935 1101 46-47 0.11090816487627109 1101 48-49 -0.4114144211957367 1101 50-51 -0.5363267722072891 1101 52-53 -0.39205363096403545 1101 54-55 -0.18336268348209472 1101 56-57 -0.0601902164535133 1101 58-59 -0.2869588366029987 1101 60-61 -0.07822285105326188 1101 62-63 -0.31566240723257977 1101 64-65 -0.4373802387645185 1101 66-67 -0.4341593823962526 1101 68-69 -0.49499547405422106 1101 70-71 -0.5081071772229961 1101 72-73 -0.4697799420141351 1101 74-75 -0.5682717523186938 1101 76-77 -0.6858801933521477 1101 78-79 -0.7114141554060218 1101 80-81 -0.7426750951580665 1101 82-83 -1.015133245259495 1101 84-85 -1.050817635786295 1101 86-87 -1.4510459744607154 1101 88-89 -1.0038157781935162 1101 90-91 -0.8182702079532511 1101 92-93 -1.3421590927840157 1101 94-95 -1.5829896857767878 1101 96-97 -1.4287715042970888 1101 98-99 -1.4335971587398326 1101 100-101 -1.436965075047695 2304 1 -0.028826324028656813 2304 2 -0.16023605078078162 2304 3 0.03236885957219471 2304 4 -0.010270741302022657 2304 5 -0.04005813911845735 2304 6 -0.25438613132831733 2304 7 -0.24554587696098196 2304 8 -0.15001073946695698 2304 9 -0.18526160419256854 2304 10-11 -0.25630194387176886 2304 12-13 -0.2957925321856578 2304 14-15 -0.4153560991981209 2304 16-17 -0.5824648851241889 2304 18-19 -0.401691320806151 2304 20-21 -0.5474530210194644 2304 22-23 -0.44752988239361713 2304 24-25 -0.6548162295140187 2304 26-27 -0.3160360638132502 2304 28-29 -0.36149485002203363 2304 30-31 -0.3408695939463797 2304 32-33 -0.1837911919935209 2304 34-35 -0.20419120052671502 2304 36-37 -0.26231285090938883 2304 38-39 -0.3779875738791958 2304 40-41 -0.35779612236122205 2304 42-43 -0.43871147722402526 2304 44-45 -0.1223104055924864 2304 46-47 -0.5605416984697698 2304 48-49 -0.7677095641553748 2304 50-51 -0.6817365676516616 2304 52-53 -0.6076469754170688 2304 54-55 -0.7561110367926744 2304 56-57 -0.45560093648487765 2304 58-59 -0.7749040714665867 2304 60-61 -0.690782070849778 2304 62-63 -0.7737175939806633 2304 64-65 -0.6429547586264945 2304 66-67 -0.4930833110773065 2304 68-69 -0.6477132050437859 2304 70-71 -0.46901933776089066 2304 72-73 -0.7906227215445121 2304 74-75 -0.6042418844606665 2304 76-77 -0.5795101588465634 2304 78-79 -0.5698118091740838 2304 80-81 -0.6285681167453241 2304 82-83 -0.4447019069383238 2304 84-85 -0.6360534609431987 2304 86-87 -0.6767228645938914 2304 88-89 -0.5397970053793308 2304 90-91 -0.47959769296569377 2304 92-93 -0.656106302565405 2304 94-95 -0.5387149133176443 2304 96-97 -0.9349606895880207 2304 98-99 -0.928441661700365 2304 100-101 -0.5511977972906692 2202 1 0.07915727932874717 2202 2 0.16625588398755298 2202 3 0.15085767117051319 2202 4 0.04981479096777264 2202 5 0.08366553131612164 2202 6 -0.0323535864771074 2202 7 0.07085963827334041 2202 8 -0.12624980393557195 2202 9 -0.05170704631632361 2202 10-11 -0.02095156323542824 2202 12-13 0.09587960594363665 2202 14-15 0.20402175113815701 2202 16-17 0.15841315153756597 2202 18-19 0.033999769286651826 2202 20-21 0.19037282676148948 2202 22-23 0.10584615792367202 2202 24-25 0.10633224527880003 2202 26-27 0.3434922665329623 2202 28-29 0.2519649480366368 2202 30-31 0.07784458276115913 2202 32-33 0.10037291639467583 2202 34-35 -0.015754027705391138 2202 36-37 -0.1354535887583026 2202 38-39 0.18701517663985356 2202 40-41 0.10001446447532913 2202 42-43 0.16719237882622195 2202 44-45 -0.08682338626788066 2202 46-47 0.07484417273496291 2202 48-49 0.048065993490176595 2202 50-51 0.3590527425891352 2202 52-53 0.18169520689798446 2202 54-55 0.18129779680979397 2202 56-57 0.3059876213558752 2202 58-59 0.1931295735922376 2202 60-61 0.3206299214132784 2202 62-63 0.09899193346242185 2202 64-65 0.1749147425535611 2202 66-67 0.3906726557745017 2202 68-69 0.353631887497869 2202 70-71 0.22382398912499468 2202 72-73 0.09054598287229254 2202 74-75 -0.04223986149826686 2202 76-77 -0.15892385465689784 2202 78-79 -0.20333620006720565 2202 80-81 0.17143720683993635 2202 82-83 0.08208814860441649 2202 84-85 0.3380118062120552 2202 86-87 -0.18197901777118375 2202 88-89 -0.233708598373493 2202 90-91 -0.04153050931975244 2202 92-93 0.007943526192605077 2202 94-95 -0.10763227352916971 2202 96-97 -0.29103578763071525 2202 98-99 -0.1940371047113807 2202 100-101 -0.08397333719094036 1102 1 0.19213189929794083 1102 2 0.14907581300382589 1102 3 0.1702643543554032 1102 4 0.18714486529979268 1102 5 0.1483745649999122 1102 6 0.24823638204897236 1102 7 0.08824395488755243 1102 8 0.28823489438358507 1102 9 0.04645139472030735 1102 10-11 0.23416629653822696 1102 12-13 0.14729928048994623 1102 14-15 0.18718487977942289 1102 16-17 0.09862442857500042 1102 18-19 0.29496541584126135 1102 20-21 0.06979591656259743 1102 22-23 0.14979077722320966 1102 24-25 0.21318451876704358 1102 26-27 0.2296320241168317 1102 28-29 0.23925484290035826 1102 30-31 0.003576183912976205 1102 32-33 0.014882726881516817 1102 34-35 0.2373263981969771 1102 36-37 0.23050435673155079 1102 38-39 0.4711861402774318 1102 40-41 0.365073394362156 1102 42-43 0.13983685749762031 1102 44-45 0.20293419760676557 1102 46-47 0.3096580078652096 1102 48-49 0.2711022219774222 1102 50-51 0.1503890447387377 1102 52-53 0.059906216072292295 1102 54-55 0.2168114243461261 1102 56-57 0.2236247337914179 1102 58-59 0.348592307135398 1102 60-61 0.21643284112130345 1102 62-63 0.4496548946892318 1102 64-65 0.4058529331381706 1102 66-67 0.47736488104738584 1102 68-69 0.37129913453463104 1102 70-71 0.2935320183220753 1102 72-73 0.36382496231379235 1102 74-75 0.40797945143282277 1102 76-77 0.6534346801318165 1102 78-79 0.3456994926937895 1102 80-81 0.3411653257711613 1102 82-83 0.41603310273285743 1102 84-85 0.5120391282504961 1102 86-87 0.8536601706035576 1102 88-89 0.38580020762663736 1102 90-91 0.2625259428332001 1102 92-93 0.4739198872720891 1102 94-95 0.6622197320959593 1102 96-97 0.3384107401935701 1102 98-99 0.06681804830542504 1102 100-101 0.03869123121460305 2305 1 0.12740687360556535 2305 2 0.20011141341490202 2305 3 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14840.0 31 18745.0 32 23973.0 33 31836.0 34 43827.0 35 66458.0 36 117920.0 37 144738.0 38 24475.0 39 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.84703278876418 16.509526286555637 8.616947180025216 25.026493744654964 2 21.130893080385874 16.60598067099609 31.991423437783777 30.271702810834256 3 25.94881578761761 20.151888109915223 26.314341735904417 27.58495436656275 4 15.378240461555281 13.41112909653234 33.86707715745707 37.343553284455304 5 16.737876540945827 33.547168680116314 32.73814456985768 16.976810209080185 6 36.35281482617966 33.42256692960598 14.929697681513657 15.294920562700709 7 28.77876368407964 26.755627514369717 19.212650641720945 25.252958159829696 8 29.995625685691408 30.991664109103866 16.62556927820286 22.387140927001866 9 24.342171403173662 12.28634570239815 17.60841230451216 45.763070589916026 10-11 31.737383981128332 20.428404506272184 23.16294450935867 24.671267003240814 12-13 28.775439831166917 18.187831212777837 23.885859523891416 29.15086943216383 14-15 27.366979483208965 19.3058541549456 19.471462912633005 33.855703449212434 16-17 28.04922460489548 23.416341362547364 20.853000748633015 27.68143328392414 18-19 27.668994585824386 23.113062800910004 22.078902350845635 27.13904026241998 20-21 27.699935806092075 22.204214224241312 22.2040378673514 27.89181210231521 22-23 28.338124548067935 21.83726918396501 21.80614098516781 28.018465282799244 24-25 28.18778332421992 21.901292928580247 21.761240364771663 28.149683382428165 26-27 27.967461176269303 22.24247667577676 21.870312115543868 27.919750032410068 28-29 28.242855114625925 21.96900686335806 21.802956455639496 27.98518156637651 30-31 28.097597195081512 21.993054722718007 21.850992232859028 28.05835584934145 32-33 28.00732351338146 22.282389172771065 21.77863304999991 27.931654263847562 34-35 28.289051854530385 21.913312666193352 21.73586336756788 28.06177211170839 36-37 28.27685113236354 21.902857611580387 21.887951708401726 27.93233954765435 38-39 27.922667224058145 22.090958880913135 22.078435211619144 27.90793868340958 40-41 28.02035695227847 21.947052519702055 21.97871692987524 28.053873598144236 42-43 27.789395881164296 21.70578258142987 22.24046034149874 28.264361195907096 44-45 27.57020061350018 21.738862008322293 22.661405405786798 28.029531972390725 46-47 27.65857801697108 21.625032743552495 22.77002075301792 27.9463684864585 48-49 27.55272769287624 21.674867723234406 22.74269556190394 28.029709021985415 50-51 27.050436110995957 22.064861422632966 22.964513997976663 27.920188468394414 52-53 26.976348297015644 21.911805564743396 22.891743734261233 28.22010240397973 54-55 27.03347488832149 21.806700269439556 22.954045341694336 28.205779500544615 56-57 26.596133120015804 22.202015462580942 23.09121088272181 28.11064053468144 58-59 26.900281523188617 22.010403657684734 22.9494316618335 28.139883157293145 60-61 26.647414088899986 21.87746151763119 23.09603678571334 28.379087607755483 62-63 26.58208084990398 21.915688804875447 23.139842689433802 28.36238765578677 64-65 26.792132953335607 21.770658847957687 22.9592543294425 28.4779538692642 66-67 26.732328266833687 21.639481646803517 22.927072398309793 28.701117688053003 68-69 26.582174640572227 21.95263730321776 22.845131373398242 28.62005668281177 70-71 26.704591564900444 21.719075829801948 22.753262929102963 28.823069676194645 72-73 26.47497322561237 21.497495469967696 22.90087918796414 29.12665211645579 74-75 26.264145550525242 21.73351141347881 23.08402112966799 28.91832190632796 76-77 26.262916796069906 21.884087636579235 22.84031875547023 29.012676811880628 78-79 26.237920281577075 21.563581992122547 22.922156111201776 29.276341615098602 80-81 26.1147216029937 21.690592362773128 23.019480777255048 29.17520525697812 82-83 26.188997713737333 21.544056422704564 23.151320246126055 29.115625617432045 84-85 26.223459804260546 21.536191336376913 23.255526781871165 28.984822077491373 86-87 26.111064556107845 21.72890580550692 23.2876555243392 28.872374114046035 88-89 26.43094554256318 21.45650885277368 23.16681603590507 28.945729568758072 90-91 26.621887431530112 21.22959134170114 23.245627188610843 28.902894038157907 92-93 26.442582961698445 21.358000185266054 23.489089152672463 28.71032770036303 94-95 26.66462570569755 21.112211975264962 23.35403408875357 28.86912823028392 96-97 27.04411948947524 20.82490016957497 23.209095994254568 28.921884346695233 98-99 27.077381341022473 20.81087090404403 23.3425153568019 28.769232398131596 100-101 27.396803379798502 20.756501682663885 23.09290739966223 28.75378753787538 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2.0 1 2.0 2 7.0 3 8.5 4 6.5 5 8.5 6 9.0 7 10.0 8 14.5 9 16.5 10 13.0 11 15.0 12 18.0 13 19.5 14 24.5 15 23.0 16 24.5 17 39.0 18 53.5 19 82.5 20 115.5 21 199.0 22 269.0 23 309.0 24 374.0 25 447.0 26 600.5 27 793.0 28 1035.5 29 1220.5 30 1373.5 31 1577.5 32 1802.0 33 2087.5 34 2356.5 35 2682.5 36 3080.5 37 3431.5 38 3829.0 39 4317.0 40 4776.5 41 5253.5 42 5856.0 43 6746.0 44 7828.5 45 9038.5 46 10588.5 47 12193.5 48 13787.0 49 15881.0 50 17753.5 51 19147.0 52 20647.0 53 22174.5 54 23449.5 55 24248.5 56 24605.0 57 24711.0 58 24500.5 59 24026.0 60 23715.0 61 22938.5 62 21844.0 63 20702.0 64 19239.5 65 17858.5 66 16369.5 67 14564.5 68 12704.5 69 10857.0 70 8949.5 71 7091.0 72 5597.0 73 4328.0 74 3173.0 75 2271.5 76 1593.0 77 1108.0 78 757.5 79 542.5 80 358.0 81 231.0 82 179.5 83 141.0 84 101.0 85 88.0 86 77.5 87 49.0 88 32.5 89 28.5 90 33.0 91 29.5 92 19.5 93 10.0 94 9.0 95 11.0 96 8.0 97 4.0 98 3.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0022922514846736536 2 1.7632703728258877E-4 3 0.0 4 3.5265407456517753E-4 5 0.004408175932064719 6 0.013577181870759333 7 0.02415680410771466 8 0.032091520785431156 9 0.026272728555105727 10-11 0.02477394873820372 12-13 0.0312980491176595 14-15 0.02274618780945395 16-17 0.01666290502320464 18-19 0.0172800496536937 20-21 0.01692739557912852 22-23 0.020806590399345477 24-25 0.03491275338195258 26-27 0.03076906800581174 28-29 0.023187005402660422 30-31 0.021864552623041007 32-33 0.032973155971844095 34-35 0.03623520616157199 36-37 0.04205399839189742 38-39 0.035529898012441635 40-41 0.04328828765287554 42-43 0.043111960615592956 44-45 0.038262967090321766 46-47 0.039232765795376 48-49 0.04046705505635412 50-51 0.042759306541027775 52-53 0.045051558025701426 54-55 0.03711684134798493 56-57 0.03720500486662623 58-59 0.03738133190390882 60-61 0.04893075284591838 62-63 0.050694023218744264 64-65 0.049812388032331324 66-67 0.05871690341510206 68-69 0.051046677293309445 70-71 0.053956073408472166 72-73 0.062596098235319 74-75 0.06030384675064535 76-77 0.07476266380781764 78-79 0.05651281544906969 80-81 0.05968670212015629 82-83 0.046550337842603436 84-85 0.04443441339521237 86-87 0.05210463951700498 88-89 0.05289811118477663 90-91 0.04813728117814673 92-93 0.06621080249961209 94-95 0.07079530546895939 96-97 0.07352837454683951 98-99 0.07776022344162165 100-101 0.08234472641096896 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 567128.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.37556244558927 #Duplication Level Percentage of deduplicated Percentage of total 1 92.95127942497541 83.07572878644307 2 5.8510375493432205 10.458795437256253 3 0.7172641442583475 1.9231765894543225 4 0.18059336900200254 0.6456253571399129 5 0.051353420234862404 0.22948704084977664 6 0.045492213612028036 0.2439535307081939 7 0.03306869809549717 0.20688734441399126 8 0.02731803468881058 0.19532517721764509 9 0.01467215130185754 0.11801985974012723 >10 0.1171793029758874 2.016260208791337 >50 0.008049263472769939 0.5186118056923268 >100 0.002692428039226847 0.36812886229303726 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-11 0.0 0.0 0.0 12-13 0.0 0.0 0.0 14-15 0.0 0.0 0.0 16-17 0.0 0.0 0.0 18-19 0.0 0.0 8.816351864129438E-5 20-21 0.0 0.0 3.5265407456517753E-4 22-23 0.0 0.0 5.289811118477663E-4 24-25 0.0 1.7632703728258877E-4 8.816351864129439E-4 26-27 0.0 1.7632703728258877E-4 0.003967358338858247 28-29 0.0 1.7632703728258877E-4 0.023187005402660422 30-31 8.816351864129438E-5 1.7632703728258877E-4 0.04196583487325613 32-33 1.7632703728258877E-4 1.7632703728258877E-4 0.05986302915743888 34-35 1.7632703728258877E-4 1.7632703728258877E-4 0.12175381924362753 36-37 1.7632703728258877E-4 1.7632703728258877E-4 0.33678464120974455 38-39 1.7632703728258877E-4 1.7632703728258877E-4 0.7441000973325246 40-41 1.7632703728258877E-4 1.7632703728258877E-4 1.2173618653989928 42-43 1.7632703728258877E-4 1.7632703728258877E-4 1.7203347392475772 44-45 1.7632703728258877E-4 1.7632703728258877E-4 2.355376564020821 46-47 1.7632703728258877E-4 1.7632703728258877E-4 3.1149934406342132 48-49 1.7632703728258877E-4 1.7632703728258877E-4 3.862531915193748 50-51 1.7632703728258877E-4 1.7632703728258877E-4 4.58529644101508 52-53 1.7632703728258877E-4 1.7632703728258877E-4 5.302418501643368 54-55 1.7632703728258877E-4 1.7632703728258877E-4 6.072967654568281 56-57 1.7632703728258877E-4 1.7632703728258877E-4 6.936441156141118 58-59 1.7632703728258877E-4 1.7632703728258877E-4 7.768087627484448 60-61 2.6449055592388314E-4 3.5265407456517753E-4 8.566672779337292 62-63 3.5265407456517753E-4 3.5265407456517753E-4 9.365169767671496 64-65 3.5265407456517753E-4 3.5265407456517753E-4 10.173805560649448 66-67 3.5265407456517753E-4 3.5265407456517753E-4 11.029961490175058 68-69 3.5265407456517753E-4 3.5265407456517753E-4 11.90948075214061 70-71 3.5265407456517753E-4 3.5265407456517753E-4 12.752235826832743 72-73 3.5265407456517753E-4 3.5265407456517753E-4 13.563692852407218 74-75 3.5265407456517753E-4 3.5265407456517753E-4 14.372240481866527 76-77 3.5265407456517753E-4 3.5265407456517753E-4 15.198861632647304 78-79 3.5265407456517753E-4 3.5265407456517753E-4 16.027334217319545 80-81 3.5265407456517753E-4 3.5265407456517753E-4 16.84143614845326 82-83 3.5265407456517753E-4 3.5265407456517753E-4 17.628560042882736 84-85 3.5265407456517753E-4 3.5265407456517753E-4 18.385972831530097 86-87 3.5265407456517753E-4 3.5265407456517753E-4 19.162428940203974 88-89 3.5265407456517753E-4 3.5265407456517753E-4 19.963835324653342 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCCTAG 25 0.0050228634 56.991695 1 TAGTACA 60 8.288247E-5 39.581055 4 CTATACT 75 6.9188136E-6 37.997814 4 GTATATA 90 5.697675E-7 36.939056 1 GTAGTAC 100 1.2982382E-6 33.248085 3 CTTACAC 100 1.2982382E-6 33.248085 3 TAGAGGT 60 0.003950392 31.664845 4 ATAATAG 80 4.4923142E-4 29.685791 3 CCCCGTA 100 4.9477072E-5 28.500874 9 CTATAGT 150 3.3322067E-8 28.49836 3 TCTATAC 150 3.3322067E-8 28.49836 3 TATAGGG 260 0.0 27.402267 2 TATACTG 90 8.94494E-4 26.38737 5 ATATACT 145 7.8796984E-7 26.205389 4 AGGACAG 150 1.0590556E-6 25.331877 5 CAATACA 150 1.0590556E-6 25.331877 4 CTATAAC 115 1.2811343E-4 24.781183 3 TAGGGAG 115 1.2811343E-4 24.781183 5 GTCTATA 115 1.2819016E-4 24.778997 1 TTCCTAG 50 0.0016533368 23.750729 60-61 >>END_MODULE