FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L59_TCCTGAGC-GTAAGGAG_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L59_TCCTGAGC-GTAAGGAG_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567128
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTAG250.005022863456.9916951
TAGTACA608.288247E-539.5810554
CTATACT756.9188136E-637.9978144
GTATATA905.697675E-736.9390561
GTAGTAC1001.2982382E-633.2480853
CTTACAC1001.2982382E-633.2480853
TAGAGGT600.00395039231.6648454
ATAATAG804.4923142E-429.6857913
CCCCGTA1004.9477072E-528.5008749
CTATAGT1503.3322067E-828.498363
TCTATAC1503.3322067E-828.498363
TATAGGG2600.027.4022672
TATACTG908.94494E-426.387375
ATATACT1457.8796984E-726.2053894
AGGACAG1501.0590556E-625.3318775
CAATACA1501.0590556E-625.3318774
CTATAAC1151.2811343E-424.7811833
TAGGGAG1151.2811343E-424.7811835
GTCTATA1151.2819016E-424.7789971
TTCCTAG500.001653336823.75072960-61
Produced by FastQC (version 0.11.2)