##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename G140_A6_L59_TCCTGAGC-GTAAGGAG_L006_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 567128 Sequences flagged as poor quality 0 Sequence length 101 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74110430096909 33.0 33.0 33.0 33.0 33.0 2 32.7683891467182 33.0 33.0 33.0 33.0 33.0 3 32.77699214286722 33.0 33.0 33.0 33.0 33.0 4 36.74667623534722 37.0 37.0 37.0 37.0 37.0 5 36.63402794430887 37.0 37.0 37.0 37.0 37.0 6 36.632904741081376 37.0 37.0 37.0 37.0 37.0 7 36.592806209532945 37.0 37.0 37.0 37.0 37.0 8 36.60446142669733 37.0 37.0 37.0 37.0 37.0 9 36.65214554738966 37.0 37.0 37.0 37.0 37.0 10-11 36.629431098446915 37.0 37.0 37.0 37.0 37.0 12-13 36.60567543834901 37.0 37.0 37.0 37.0 37.0 14-15 38.89201732236815 40.0 40.0 40.0 37.0 40.0 16-17 38.81279711105782 40.0 38.5 40.0 37.0 40.0 18-19 38.74096676588002 40.0 37.0 40.0 37.0 40.0 20-21 38.68938140948781 40.0 37.0 40.0 37.0 40.0 22-23 38.63881610500628 40.0 37.0 40.0 37.0 40.0 24-25 38.55105725691554 40.0 37.0 40.0 37.0 40.0 26-27 38.42342910242485 40.0 37.0 40.0 37.0 40.0 28-29 38.31442196470638 40.0 37.0 40.0 37.0 40.0 30-31 38.15661102960884 40.0 37.0 40.0 37.0 40.0 32-33 37.962723582683275 40.0 37.0 40.0 33.0 40.0 34-35 37.771265922331466 40.0 37.0 40.0 33.0 40.0 36-37 37.566918755554305 40.0 37.0 40.0 33.0 40.0 38-39 37.30173523437389 38.5 37.0 40.0 33.0 40.0 40-41 37.03229782341906 37.0 37.0 40.0 33.0 40.0 42-43 36.73886406596041 37.0 37.0 40.0 33.0 40.0 44-45 36.38852340212439 37.0 37.0 40.0 33.0 40.0 46-47 36.09800168568648 37.0 37.0 40.0 33.0 40.0 48-49 36.00047960954141 37.0 35.0 40.0 33.0 40.0 50-51 36.2247649560593 37.0 37.0 38.5 33.0 40.0 52-53 36.106260844112796 37.0 37.0 37.0 33.0 40.0 54-55 35.88355626948413 37.0 37.0 37.0 33.0 40.0 56-57 35.677336509571035 37.0 37.0 37.0 33.0 40.0 58-59 35.488577534524836 37.0 33.0 37.0 33.0 40.0 60-61 35.333012124247084 37.0 33.0 37.0 33.0 38.5 62-63 35.14949887856004 37.0 33.0 37.0 33.0 37.0 64-65 35.01284013485492 37.0 33.0 37.0 33.0 37.0 66-67 34.854047939794896 37.0 33.0 37.0 33.0 37.0 68-69 34.71940725903147 37.0 33.0 37.0 33.0 37.0 70-71 34.60741049639587 37.0 33.0 37.0 30.0 37.0 72-73 34.48761390726608 37.0 33.0 37.0 27.0 37.0 74-75 34.362263016461895 37.0 33.0 37.0 27.0 37.0 76-77 32.986112482543625 33.0 33.0 37.0 27.0 37.0 78-79 34.301819871351796 37.0 33.0 37.0 27.0 37.0 80-81 34.4691912584108 37.0 33.0 37.0 30.0 37.0 82-83 34.417559175353716 37.0 33.0 37.0 27.0 37.0 84-85 34.36163088403323 37.0 33.0 37.0 27.0 37.0 86-87 34.31398908182985 37.0 33.0 37.0 27.0 37.0 88-89 34.23213895275846 37.0 33.0 37.0 27.0 37.0 90-91 34.124337010339815 37.0 33.0 37.0 27.0 37.0 92-93 34.06893593686081 37.0 33.0 37.0 27.0 37.0 94-95 34.04017258890409 37.0 33.0 37.0 27.0 37.0 96-97 33.94637894796236 37.0 33.0 37.0 27.0 37.0 98-99 33.8595563964396 37.0 33.0 37.0 27.0 37.0 100-101 32.9705216459071 35.0 33.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.029893807906987035 2306 2 0.022189988878423605 2306 3 0.1476680081787336 2306 4 0.1197654611885639 2306 5 0.04653613241065102 2306 6 0.07702250875375682 2306 7 0.11973115168197523 2306 8 0.07250568540781188 2306 9 0.15879429050803395 2306 10-11 0.05539118767067919 2306 12-13 0.09866864756203597 2306 14-15 0.11510501422672093 2306 16-17 0.09937037728626308 2306 18-19 0.0609525999029259 2306 20-21 0.12597818029971108 2306 22-23 0.008883432110103229 2306 24-25 -0.006334635889821527 2306 26-27 0.02965090892529787 2306 28-29 0.13221271436377435 2306 30-31 -0.05824011915112948 2306 32-33 0.14145978540225457 2306 34-35 0.08118599028388473 2306 36-37 -0.033225754853255296 2306 38-39 0.09621425109073556 2306 40-41 -0.21755637347693835 2306 42-43 -0.017772467963077077 2306 44-45 -0.14518055596759183 2306 46-47 -0.18479856205077283 2306 48-49 -0.026461752529925775 2306 50-51 -0.15935282682906404 2306 52-53 -0.18136421963967564 2306 54-55 0.10334832728369747 2306 56-57 0.004469143847337875 2306 58-59 -0.17266101501152065 2306 60-61 -0.19539021286604452 2306 62-63 -0.29180915476764824 2306 64-65 -0.2369968112523253 2306 66-67 0.23419950087365038 2306 68-69 0.12189588632742243 2306 70-71 0.011508865590684536 2306 72-73 -0.15495912061424377 2306 74-75 -0.03070109042234037 2306 76-77 -0.06428561987871007 2306 78-79 0.12805938665864858 2306 80-81 0.1089375852997776 2306 82-83 0.11909615505542348 2306 84-85 -0.008782892484461513 2306 86-87 -0.03587315566939253 2306 88-89 -0.07890602765682075 2306 90-91 -0.12895559778982602 2306 92-93 -0.19922428377405765 2306 94-95 -0.28264717438039355 2306 96-97 -0.27407041548855915 2306 98-99 -0.225231707560269 2306 100-101 -0.20708892882980479 2307 1 0.09418987115987676 2307 2 0.08226781311156373 2307 3 0.054808795528160204 2307 4 0.06376513002161488 2307 5 0.06267224202691324 2307 6 0.06666526240694282 2307 7 0.09510060981005353 2307 8 -0.03107091764720593 2307 9 0.14973273485128402 2307 10-11 0.13133397858014462 2307 12-13 0.18968160446893734 2307 14-15 0.10274213804176924 2307 16-17 0.16058865770187936 2307 18-19 0.044741977723013804 2307 20-21 0.18165769348429706 2307 22-23 0.14870211820523593 2307 24-25 0.26609364576766836 2307 26-27 0.21945510646639121 2307 28-29 0.30481278887634033 2307 30-31 0.15698942094076784 2307 32-33 0.23895533534636826 2307 34-35 0.30399097434647615 2307 36-37 0.23506500972843725 2307 38-39 0.36739030772027803 2307 40-41 0.3168249652654538 2307 42-43 0.21289566135761362 2307 44-45 0.09950007490544266 2307 46-47 0.3145101269420678 2307 48-49 0.4126366454499504 2307 50-51 0.3450972290553622 2307 52-53 0.09777640212627148 2307 54-55 0.30305855620284206 2307 56-57 0.31137604453864753 2307 58-59 0.3696973076278809 2307 60-61 0.3166828922380702 2307 62-63 0.3599149831633781 2307 64-65 0.24383069216283104 2307 66-67 0.5336572149937808 2307 68-69 0.1976855125227246 2307 70-71 0.31487434971536743 2307 72-73 0.3438817950623658 2307 74-75 0.18931588188382165 2307 76-77 0.14180164261248507 2307 78-79 0.2154046695994083 2307 80-81 0.3901459307069146 2307 82-83 0.6124955837924375 2307 84-85 0.5029259289751593 2307 86-87 0.3272886607813277 2307 88-89 0.39042170343562077 2307 90-91 0.5513792947878926 2307 92-93 0.5549028843345738 2307 94-95 0.3969819186361221 2307 96-97 0.29173659697159593 2307 98-99 0.30716883886519497 2307 100-101 0.5923087612807265 2201 1 -0.04317305197305643 2201 2 -0.01823588561338596 2201 3 0.008119644454545494 2201 4 0.10047632541591156 2201 5 -0.04535293090697934 2201 6 0.11857143601697118 2201 7 0.1510075675737781 2201 8 0.10861646107902345 2201 9 0.11543801308582147 2201 10-11 -0.026807017054998994 2201 12-13 0.05611742588993707 2201 14-15 0.22055322901560004 2201 16-17 0.08560996187750192 2201 18-19 0.187934043367882 2201 20-21 0.11325628680859268 2201 22-23 -0.030024912292745398 2201 24-25 -0.10908188863707124 2201 26-27 0.04723735178647104 2201 28-29 0.192410758078303 2201 30-31 0.21252830656235489 2201 32-33 0.13955985383199732 2201 34-35 0.2566156887905038 2201 36-37 0.25312855018236746 2201 38-39 0.14665904439267052 2201 40-41 0.25295644703587783 2201 42-43 -0.022552486479355593 2201 44-45 0.1064094291388713 2201 46-47 0.010274496758555074 2201 48-49 0.16064125435329402 2201 50-51 0.46049984796866994 2201 52-53 0.29133633987407137 2201 54-55 0.17683381615379545 2201 56-57 0.3438424079458784 2201 58-59 0.15607343734929913 2201 60-61 0.29355435904556515 2201 62-63 -0.09318982537909903 2201 64-65 0.041316999479064975 2201 66-67 0.21036985067037506 2201 68-69 0.12892603566525906 2201 70-71 0.07758784155976883 2201 72-73 0.023400980822778195 2201 74-75 0.07315311398130575 2201 76-77 0.006863194676689943 2201 78-79 0.27765887142295753 2201 80-81 0.21236108487712357 2201 82-83 0.447617267642471 2201 84-85 0.29899595870098494 2201 86-87 0.4997344601580025 2201 88-89 0.4723337581983529 2201 90-91 0.4163348260720312 2201 92-93 0.3493225905969979 2201 94-95 0.2847239160676125 2201 96-97 0.08459399702201154 2201 98-99 0.11405098776638312 2201 100-101 0.36769873771610406 1101 1 0.006332719991895885 1101 2 0.04666807839338816 1101 3 0.03758099058011055 1101 4 0.026668337061664715 1101 5 0.15457843154828055 1101 6 0.22201802129912096 1101 7 0.16835174714769607 1101 8 0.13236052600561976 1101 9 0.011297509768965597 1101 10-11 0.00441175191932075 1101 12-13 0.16379917395997268 1101 14-15 -0.06949130996260777 1101 16-17 -0.00677353343289866 1101 18-19 0.11466157871432614 1101 20-21 0.07616938488862957 1101 22-23 -0.02030063157272366 1101 24-25 0.06991502267340621 1101 26-27 0.09811776029300034 1101 28-29 0.17812132637454425 1101 30-31 0.05875757858953534 1101 32-33 0.14378935967938844 1101 34-35 -0.01198527439790098 1101 36-37 0.08903202014966638 1101 38-39 0.26559810405830575 1101 40-41 0.017529668588068148 1101 42-43 0.0011821502130686667 1101 44-45 0.30874499130676014 1101 46-47 0.21625501425546645 1101 48-49 0.11738263034750673 1101 50-51 0.014896987819547292 1101 52-53 0.07538452192196132 1101 54-55 0.2037853663440572 1101 56-57 0.13994930149356577 1101 58-59 0.07726094229912661 1101 60-61 0.08945194579474958 1101 62-63 0.22310480316733106 1101 64-65 0.0747193474865 1101 66-67 -0.10431066418663448 1101 68-69 -0.13717267417517576 1101 70-71 -0.12204558869581206 1101 72-73 -0.03695857431542038 1101 74-75 -0.07247539096474043 1101 76-77 -0.14151081205883997 1101 78-79 -0.1316471728293962 1101 80-81 0.04773845937790355 1101 82-83 0.3508458721506784 1101 84-85 0.18910800383582682 1101 86-87 -0.016893173619216384 1101 88-89 0.07819585707412813 1101 90-91 0.031572361003640026 1101 92-93 0.14997577866009948 1101 94-95 0.26348776142949504 1101 96-97 0.04021529879715757 1101 98-99 -0.021200802655286566 1101 100-101 0.009227846446272281 2304 1 -0.050799359108367526 2304 2 0.019414952364783744 2304 3 6.344945798701929E-4 2304 4 0.04148022059329293 2304 5 0.128926690181153 2304 6 0.036490396489121224 2304 7 0.20041642385660907 2304 8 0.042390815475307875 2304 9 0.14292171323727842 2304 10-11 0.12107428876411319 2304 12-13 0.17763284445936733 2304 14-15 0.019642573931456297 2304 16-17 0.13152803725547102 2304 18-19 0.21003087724867697 2304 20-21 0.1802334994486543 2304 22-23 0.1585459276692589 2304 24-25 0.2127452204240754 2304 26-27 0.22822194275469343 2304 28-29 0.0902911101321422 2304 30-31 -0.11051580698912744 2304 32-33 0.20932778366538685 2304 34-35 0.05330536027559418 2304 36-37 -0.016966330387148787 2304 38-39 0.03858980866385764 2304 40-41 0.19646683735478376 2304 42-43 -0.058440766230155816 2304 44-45 0.2825717828789678 2304 46-47 0.399210438067648 2304 48-49 0.027270533421202003 2304 50-51 0.061623764968196326 2304 52-53 0.09532231570947403 2304 54-55 -0.020221719690610485 2304 56-57 -0.05598362526804834 2304 58-59 0.13187259727285294 2304 60-61 -0.06663128182747613 2304 62-63 -0.09509735592818913 2304 64-65 0.01722298111336329 2304 66-67 -0.03621853094255556 2304 68-69 -0.07195600646064548 2304 70-71 -0.16444344957135826 2304 72-73 0.06623457930285781 2304 74-75 0.13992812838316837 2304 76-77 -0.021908088595409936 2304 78-79 0.059492694991647 2304 80-81 0.08712216667585437 2304 82-83 -0.22865381533892304 2304 84-85 0.10137579381404294 2304 86-87 0.18046916462429863 2304 88-89 0.25843854266647526 2304 90-91 0.11600749382980524 2304 92-93 0.16743383014849655 2304 94-95 0.08391817516367439 2304 96-97 0.28296191388420056 2304 98-99 0.08571843652057964 2304 100-101 0.30727770757331285 2202 1 0.13043677738835413 2202 2 -0.013889397004369641 2202 3 0.07985299226411513 2202 4 0.05588229324249028 2202 5 0.012609901367781617 2202 6 0.0905780227024593 2202 7 0.05315185941461209 2202 8 0.1443836108899319 2202 9 -0.1278748725700467 2202 10-11 -0.06712332272063293 2202 12-13 -0.031718920309145915 2202 14-15 -0.001848739751928008 2202 16-17 0.011868036900573031 2202 18-19 0.10373485041780839 2202 20-21 -0.029526406856248855 2202 22-23 0.04477558713725216 2202 24-25 0.046810842485008664 2202 26-27 -0.02475837772514211 2202 28-29 -0.030753022389639284 2202 30-31 0.006560479987605561 2202 32-33 -0.10343817378141296 2202 34-35 -0.03663838717351808 2202 36-37 -0.04288686700453326 2202 38-39 -0.016667231777645952 2202 40-41 -0.06939123586226259 2202 42-43 -0.03760957711866553 2202 44-45 0.030975594566008624 2202 46-47 -0.025315319212353415 2202 48-49 0.0727144317052364 2202 50-51 0.05328525592697275 2202 52-53 -0.0728639387933896 2202 54-55 0.06612683318146395 2202 56-57 -0.07670479361755866 2202 58-59 -0.010247609282252768 2202 60-61 -0.09088731078777812 2202 62-63 -0.04410273274930887 2202 64-65 -0.09929787846820659 2202 66-67 -0.30843296495672234 2202 68-69 -0.17961132206052355 2202 70-71 -0.11983357964429331 2202 72-73 -0.42219372087637197 2202 74-75 -0.2543951552042216 2202 76-77 -0.25905251746343794 2202 78-79 -0.08364254181916664 2202 80-81 -0.06478456411043965 2202 82-83 -0.12890047478916955 2202 84-85 -0.2335628962290741 2202 86-87 -0.05719875012736253 2202 88-89 -0.014489251772154432 2202 90-91 0.1329897308003325 2202 92-93 -0.3712988016546035 2202 94-95 -0.39968518224409166 2202 96-97 -0.14235350854229267 2202 98-99 -0.22184282907309694 2202 100-101 -0.17028870415277453 1102 1 -0.01117575860575215 1102 2 0.06407886121567685 1102 3 0.04777973170025973 1102 4 0.10804193966316689 1102 5 0.0473852311742462 1102 6 0.02096709388708007 1102 7 0.027708211118884662 1102 8 0.0781812403012978 1102 9 0.11736720872641371 1102 10-11 -0.020942046352857346 1102 12-13 -0.03398068954506783 1102 14-15 -0.0406152300753817 1102 16-17 -0.04845473816137513 1102 18-19 0.07888050099035837 1102 20-21 0.04878839927781797 1102 22-23 0.005218900604077703 1102 24-25 -0.021350277182840216 1102 26-27 0.013994719442209202 1102 28-29 0.039649776779981494 1102 30-31 -0.035567692516245586 1102 32-33 0.24473700870033355 1102 34-35 0.22466878486157071 1102 36-37 0.03885826123492819 1102 38-39 -0.08413358147294758 1102 40-41 0.06291807907780367 1102 42-43 -0.04439990725996523 1102 44-45 0.16071844596894636 1102 46-47 0.3016568230229666 1102 48-49 0.18252826683548307 1102 50-51 0.014153802099883706 1102 52-53 0.003396692024260517 1102 54-55 0.09088743051622572 1102 56-57 0.0739313812970579 1102 58-59 0.04430606379080615 1102 60-61 -0.03953962445549308 1102 62-63 0.06366529778709662 1102 64-65 0.29693362223493125 1102 66-67 0.21029066726720913 1102 68-69 -0.06506970282795521 1102 70-71 -0.04510144276623862 1102 72-73 0.12840897114800498 1102 74-75 0.14640676001710062 1102 76-77 0.24311047724626889 1102 78-79 0.27985441123782095 1102 80-81 0.30229003576902613 1102 82-83 0.41213950378178055 1102 84-85 0.3092232150680516 1102 86-87 0.08441211172223717 1102 88-89 0.2020898013317165 1102 90-91 0.5419664936744937 1102 92-93 0.5215860841687388 1102 94-95 0.36474594345067857 1102 96-97 0.4918576345635799 1102 98-99 0.13484202996393435 1102 100-101 0.6472161508663064 2305 1 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-0.6308743622727349 1213 66-67 -0.6127392746365601 1213 68-69 -0.4690479912236043 1213 70-71 -0.5818584813480925 1213 72-73 -0.6230713655122102 1213 74-75 -0.4836347638917289 1213 76-77 -0.9929845994705531 1213 78-79 -0.8164559194638059 1213 80-81 -0.8728226187818535 1213 82-83 -0.4420007837200828 1213 84-85 -2.571027790443644 1213 86-87 -1.2306435638326576 1213 88-89 -2.0519927623507 1213 90-91 -2.6497464141163576 1213 92-93 -1.957004896018951 1213 94-95 -1.4253767662171342 1213 96-97 -1.7697336807946797 1213 98-99 -1.0774000749072101 1213 100-101 -0.705685867605311 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 8.0 14 19.0 15 36.0 16 95.0 17 154.0 18 238.0 19 408.0 20 601.0 21 817.0 22 1113.0 23 1502.0 24 1877.0 25 2482.0 26 3140.0 27 4025.0 28 5124.0 29 6361.0 30 8327.0 31 11081.0 32 15178.0 33 21714.0 34 31909.0 35 58535.0 36 141473.0 37 214380.0 38 36504.0 39 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.531765494610184 16.52546708773663 8.837882182742012 25.104885234911166 2 21.069021580044367 16.16969201090464 32.22106232079817 30.540224088252828 3 26.23652121778538 19.60782584928451 26.36418942152549 27.79146351140461 4 15.442898252246406 13.147649207938949 33.7914897518726 37.61796278794205 5 16.348690242767063 33.5210040766811 33.14560381430647 16.984701866245363 6 36.47730318376098 33.43548546359904 14.800538855425934 15.286672497214033 7 28.81765668420533 26.5433905573345 19.142592148509685 25.496360609950486 8 30.053709215556278 31.215528064211256 16.549174084157368 22.1815886360751 9 24.199298923699764 11.994117730036253 17.616305313791596 46.190278032472385 10-11 31.88936183718667 20.336767008506015 23.093904726975214 24.6799664273321 12-13 28.802404395480387 17.987209236715522 23.943007575009524 29.267378792794574 14-15 27.47651323863396 19.129014966638923 19.42233499315851 33.9721368015686 16-17 28.017484589016938 23.506157340141907 20.845470511066285 27.630887559774862 18-19 27.68175791003089 23.069130778237014 22.037264956059303 27.21184635567279 20-21 27.671619105387148 22.10585617356223 22.217206697606183 28.005318023444442 22-23 28.289557207543975 21.76730826197966 21.86058526470215 28.082549265774215 24-25 28.183320167581215 21.80195652480569 21.896555980307795 28.1181673273053 26-27 27.85261880915772 22.205745440182817 21.917627061263065 28.024008689396396 28-29 28.217968430407247 21.944076116855456 21.891706986782523 27.946248465954778 30-31 27.941928453541355 21.99036196414213 21.985072153023655 28.082637429292856 32-33 27.91459776276255 22.20389400629135 21.915334809778393 27.966173421167706 34-35 28.238510530250664 21.83819173096726 21.92000747626638 28.003290262515694 36-37 28.191343047777572 21.824173731503294 21.953245122794147 28.031238097924984 38-39 27.771243881451806 21.985689297654147 22.27089828045873 27.972168540435316 40-41 27.87871521067554 21.799664273321014 22.18899437164097 28.132626144362472 42-43 27.71111636173844 21.617077626214893 22.358444654469537 28.313361357577126 44-45 27.47906998067456 21.55157213186441 22.78965242414411 28.179705463316925 46-47 27.644288414608344 21.500172800496536 22.842109717735678 28.01342906715944 48-49 27.282289007067188 21.69510234021244 22.920134431733224 28.10247422098715 50-51 26.908916505621306 22.043789056438758 23.154473064281785 27.89282137365815 52-53 26.850199602206203 21.82514353020835 22.979292152741532 28.345364714843917 54-55 26.886787462442342 21.743944929539715 23.13049258721135 28.23877502080659 56-57 26.330123005741207 22.025186554005444 23.265470934251177 28.379219506002173 58-59 26.62379568633536 21.934818947398117 22.964745172165717 28.476640194100806 60-61 26.400565657135605 21.948396129268875 23.08138550732815 28.56965270626737 62-63 26.244163575065947 21.844098686716226 23.11444682681864 28.79729091139919 64-65 26.233672116347634 21.797019367761774 22.852865667009915 29.116442848880673 66-67 26.390338688973213 21.723579156733578 22.7006072703164 29.18547488397681 68-69 26.143480836777588 22.13159992100549 22.659258580073637 29.065660662143287 70-71 26.20634142556883 22.104357393745328 22.46891354332708 29.220387637358762 72-73 25.929331649997884 21.766602953830528 22.640127096528474 29.663938299643117 74-75 25.86779351398626 22.0630968670212 22.7127738358889 29.35633578310364 76-77 26.043591570157005 22.21077076074537 22.39873538248861 29.346902286609016 78-79 25.95921908281728 22.01513591288034 22.34495563611742 29.68068936818496 80-81 25.55798690948075 22.1284260343344 22.505501403563215 29.808085652621628 82-83 25.63830387496297 21.943370808706327 22.609975173153153 29.808350143177552 84-85 25.73396129268878 21.870371415271332 22.673012088981675 29.722655203058217 86-87 25.572533890056565 22.241716155788463 22.631310744664344 29.554439209490624 88-89 25.783597353683824 21.86675671100704 22.632280543369397 29.717365391939737 90-91 25.671123336463143 21.627059170997704 22.706357917752182 29.995459574786977 92-93 25.386597029242076 21.593978784330876 23.046296426908917 29.973127759518132 94-95 25.535064394634016 21.47116700286355 23.09487452568027 29.898894076822163 96-97 25.681503999097206 21.288756682794713 23.058198501925492 29.97154081618259 98-99 25.346218137704362 21.26883172758178 23.405121947778984 29.97982818693487 100-101 25.41727793372925 21.10396594772256 23.504482233287725 29.974273885260473 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.5 7 0.5 8 0.0 9 0.0 10 0.5 11 0.5 12 0.5 13 1.5 14 4.5 15 4.5 16 9.0 17 25.0 18 36.0 19 56.0 20 88.5 21 165.5 22 234.5 23 279.5 24 346.0 25 413.0 26 523.5 27 739.5 28 993.5 29 1153.0 30 1307.5 31 1517.5 32 1773.0 33 2104.0 34 2366.0 35 2619.5 36 3030.5 37 3379.5 38 3761.0 39 4217.0 40 4547.5 41 5096.0 42 5724.0 43 6495.5 44 7604.5 45 8727.5 46 10106.5 47 11865.0 48 13643.5 49 15623.5 50 17469.5 51 18842.0 52 20457.0 53 22157.5 54 23503.0 55 24580.5 56 25424.0 57 25563.0 58 25204.5 59 24837.0 60 24470.5 61 23737.0 62 22550.0 63 21246.5 64 19839.5 65 18465.0 66 16848.5 67 14894.0 68 12874.5 69 10791.5 70 8840.0 71 7052.5 72 5357.0 73 3859.5 74 2698.0 75 1918.5 76 1271.0 77 755.5 78 447.0 79 262.0 80 149.5 81 78.5 82 38.5 83 27.0 84 12.0 85 8.0 86 4.0 87 1.0 88 1.5 89 1.5 90 2.0 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.056071997855863226 2 0.005289811118477663 3 0.0058187922303254295 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 8.816351864129438E-5 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 567128.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.96441218361501 #Duplication Level Percentage of deduplicated Percentage of total 1 92.53241898483753 82.3209226291405 2 6.131645839822553 10.909965357158438 3 0.7913649394067209 2.1120994997112312 4 0.21704228227639094 0.7723615624683746 5 0.09173451648605362 0.408055366806495 6 0.04089981515065746 0.21831768079780514 7 0.031151598699912793 0.19399685668423253 8 0.016470078328877903 0.11722006697173751 9 0.018019465969265062 0.14427820780364886 >10 0.11911048630330855 1.9643062133496896 >50 0.007500564593152875 0.445206788460405 >100 0.0026414281255456276 0.39326977064742746 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-11 0.0 0.0 0.0 12-13 0.0 0.0 0.0 14-15 0.0 0.0 0.0 16-17 0.0 0.0 0.0 18-19 0.0 0.0 1.7632703728258877E-4 20-21 0.0 0.0 5.289811118477663E-4 22-23 0.0 0.0 7.053081491303551E-4 24-25 0.0 0.0 0.001146125742336827 26-27 0.0 0.0 0.004320012413423425 28-29 0.0 0.0 0.023892313551790778 30-31 0.0 0.0 0.04302379709695166 32-33 0.0 0.0 0.06092099138113442 34-35 0.0 0.0 0.12492770591471414 36-37 0.0 0.0 0.3427797604773526 38-39 0.0 0.0 0.7575009521660013 40-41 1.7632703728258877E-4 0.0 1.2446043926591528 42-43 3.5265407456517753E-4 0.0 1.7611544483784964 44-45 3.5265407456517753E-4 0.0 2.4135644863240753 46-47 3.5265407456517753E-4 0.0 3.188962632774259 48-49 3.5265407456517753E-4 0.0 3.9528995218010747 50-51 3.5265407456517753E-4 0.0 4.688447757825394 52-53 3.5265407456517753E-4 0.0 5.421439251809115 54-55 3.5265407456517753E-4 0.0 6.207681511052179 56-57 3.5265407456517753E-4 0.0 7.09187343950572 58-59 3.5265407456517753E-4 0.0 7.944326501248395 60-61 3.5265407456517753E-4 0.0 8.761514155534552 62-63 3.5265407456517753E-4 0.0 9.5793189544512 64-65 3.5265407456517753E-4 0.0 10.408408683753933 66-67 3.5265407456517753E-4 0.0 11.289514889055027 68-69 3.5265407456517753E-4 0.0 12.19222115642324 70-71 4.4081759320647193E-4 0.0 13.057369764850263 72-73 5.289811118477663E-4 0.0 13.888222764525821 74-75 5.289811118477663E-4 0.0 14.724365575319858 76-77 5.289811118477663E-4 0.0 15.574702712615142 78-79 5.289811118477663E-4 0.0 16.427243937876458 80-81 5.289811118477663E-4 0.0 17.267177779972073 82-83 5.289811118477663E-4 0.0 18.084365434258228 84-85 5.289811118477663E-4 0.0 18.87413423424694 86-87 5.289811118477663E-4 0.0 19.677303889069133 88-89 5.289811118477663E-4 0.0 20.514680989124148 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTACT 50 2.8252773E-5 47.499695 4 CTAAACA 80 1.0766938E-5 35.624767 4 CCCCGTA 70 2.0521032E-4 33.928352 9 TCTAAAC 60 0.003949404 31.666462 3 GTATATG 110 2.7172573E-6 30.245743 1 TTAGGGT 110 2.7303704E-6 30.227076 4 TACACTG 110 2.7303704E-6 30.227076 5 TATGAGT 160 1.900844E-9 29.687307 4 ACCCTAT 100 4.928175E-5 28.517416 1 TTATACT 85 6.4033875E-4 27.940996 4 GTGCTAC 175 4.9185473E-9 27.159443 1 CTTAACT 125 7.3826395E-6 26.599827 4 TTAACAC 125 7.3826395E-6 26.599827 3 GTGTATA 90 8.9100964E-4 26.405014 1 CTATAGT 90 8.9422765E-4 26.388716 4 AGTTAGT 90 8.9422765E-4 26.388716 8 TACACCG 365 0.0 26.02723 5 GTGTACA 165 8.344978E-8 25.924923 1 GCTTATA 110 9.46875E-5 25.908922 2 TTACACT 95 0.0012258348 24.999838 4 >>END_MODULE