FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L59_TCCTGAGC-GTAAGGAG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L59_TCCTGAGC-GTAAGGAG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567128
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT502.8252773E-547.4996954
CTAAACA801.0766938E-535.6247674
CCCCGTA702.0521032E-433.9283529
TCTAAAC600.00394940431.6664623
GTATATG1102.7172573E-630.2457431
TTAGGGT1102.7303704E-630.2270764
TACACTG1102.7303704E-630.2270765
TATGAGT1601.900844E-929.6873074
ACCCTAT1004.928175E-528.5174161
TTATACT856.4033875E-427.9409964
GTGCTAC1754.9185473E-927.1594431
CTTAACT1257.3826395E-626.5998274
TTAACAC1257.3826395E-626.5998273
GTGTATA908.9100964E-426.4050141
CTATAGT908.9422765E-426.3887164
AGTTAGT908.9422765E-426.3887168
TACACCG3650.026.027235
GTGTACA1658.344978E-825.9249231
GCTTATA1109.46875E-525.9089222
TTACACT950.001225834824.9998384
Produced by FastQC (version 0.11.2)