FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L57_AGGCAGAA-GTAAGGAG_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L57_AGGCAGAA-GTAAGGAG_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556941
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGT250.005021291356.9960674
TAGGACG558.186962E-751.8146064
ATAGGAC851.6314685E-533.5270963
GTATAAC1205.378026E-627.706421
TAGGGCG700.00839074227.1409845
GACTGGT2007.166818E-1026.1255447
AGACTGG1109.472472E-525.9073036
TAGACTG1351.3418288E-524.627935
GGTTAAG1551.4093021E-624.5144371
TACAGTG1951.5672413E-824.3572945
TGGACTG1401.7782932E-523.7483625
TACACCG2402.2919266E-1023.7483625
TACACTG2303.619789E-922.7158245
ACAGTGT2103.4406185E-822.6174876
TCTAGCC1050.002195690322.6174873
CTGTATA1503.0312049E-522.1651361
GTGTACG1503.0312049E-522.1651361
GTATAGT1954.2161628E-721.9215641
TAGAGGT1754.045465E-621.7127864
ACCGTGT1553.901142E-521.4520598
Produced by FastQC (version 0.11.2)