FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L57_AGGCAGAA-GTAAGGAG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L57_AGGCAGAA-GTAAGGAG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556941
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG500.00161516137.999715
GTAGGGC902.4108711E-531.6664283
GTTATAC1205.3754593E-627.7081243
GCGTATA700.00836254427.159721
CTTAGTA700.00838455827.145092
CCTACAC1955.329639E-1026.7946683
CCTACTC1606.230039E-826.7185483
GTCTACG1351.3346713E-524.6449341
ACAGGTC1751.4833859E-724.4283878
GTATTAG1201.702089E-423.7647551
CGTATAC1201.7093614E-423.749823
ATACAGG1452.3316858E-522.930866
ATAGTAC1903.2808748E-722.4998283
ACCGTGT1503.0297684E-522.16658
CAGTCCA1503.0297684E-522.16659
GTCCAAG1302.9265537E-421.9366991
ATTAGCC1302.9390238E-421.922913
TAGTACA1954.2136708E-721.922914
ACCGACC1754.0433133E-621.714128
TAACAGG1100.002875649621.5907466
Produced by FastQC (version 0.11.2)