##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename G140_A6_L52_TAAGGCGA-GTAAGGAG_L006_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1375 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.047272727272727 27.0 15.0 33.0 6.0 33.0 2 23.053818181818183 27.0 15.0 33.0 6.0 33.0 3 23.140363636363638 27.0 15.0 33.0 6.0 33.0 4 25.125818181818182 27.0 15.0 37.0 6.0 37.0 5 24.96218181818182 27.0 15.0 37.0 6.0 37.0 6 25.556363636363635 33.0 15.0 37.0 6.0 37.0 7 25.517818181818182 33.0 15.0 37.0 6.0 37.0 8 25.672 27.0 15.0 37.0 6.0 37.0 9 25.614545454545453 33.0 15.0 37.0 6.0 37.0 10-11 25.226181818181818 30.0 15.0 37.0 6.0 37.0 12-13 25.170909090909092 27.0 15.0 37.0 6.0 37.0 14-15 25.81490909090909 27.0 15.0 37.0 6.0 40.0 16-17 25.734909090909092 27.0 15.0 37.0 6.0 40.0 18-19 25.597454545454546 27.0 15.0 37.0 6.0 40.0 20-21 25.637818181818183 27.0 15.0 37.0 6.0 40.0 22-23 25.545454545454547 27.0 15.0 37.0 6.0 40.0 24-25 25.459272727272726 27.0 15.0 37.0 6.0 40.0 26-27 25.175636363636364 27.0 15.0 37.0 6.0 40.0 28-29 25.080363636363636 27.0 15.0 37.0 6.0 40.0 30-31 24.881818181818183 27.0 15.0 37.0 6.0 40.0 32-33 24.78872727272727 27.0 15.0 37.0 6.0 40.0 34-35 24.71272727272727 27.0 15.0 37.0 6.0 40.0 36-37 24.81418181818182 27.0 15.0 37.0 6.0 40.0 38-39 24.878181818181815 27.0 15.0 37.0 6.0 40.0 40-41 24.771636363636365 27.0 15.0 37.0 6.0 40.0 42-43 24.449454545454543 27.0 15.0 37.0 6.0 40.0 44-45 24.34218181818182 27.0 15.0 37.0 6.0 40.0 46-47 24.09309090909091 27.0 15.0 37.0 6.0 40.0 48-49 24.149818181818183 27.0 15.0 37.0 6.0 40.0 50-51 23.480363636363634 27.0 15.0 35.0 6.0 38.5 52-53 24.08 27.0 15.0 37.0 6.0 37.0 54-55 24.800363636363635 27.0 15.0 37.0 6.0 40.0 56-57 24.629454545454543 27.0 15.0 37.0 6.0 40.0 58-59 24.694909090909093 27.0 15.0 37.0 6.0 40.0 60-61 24.777818181818184 27.0 15.0 37.0 6.0 40.0 62-63 24.48109090909091 27.0 15.0 37.0 6.0 38.5 64-65 24.290545454545455 27.0 15.0 37.0 6.0 40.0 66-67 24.06509090909091 27.0 15.0 37.0 6.0 37.0 68-69 24.074909090909088 27.0 15.0 37.0 6.0 37.0 70-71 23.699272727272728 27.0 15.0 35.0 6.0 37.0 72-73 23.38690909090909 27.0 15.0 33.0 6.0 37.0 74-75 23.10036363636364 27.0 10.5 33.0 6.0 37.0 76-77 22.94472727272727 27.0 15.0 33.0 6.0 37.0 78-79 22.78109090909091 27.0 15.0 33.0 6.0 37.0 80-81 22.266545454545454 22.0 10.5 33.0 6.0 37.0 82-83 22.082545454545453 24.5 10.5 33.0 6.0 37.0 84-85 22.182545454545455 24.5 10.5 33.0 6.0 37.0 86-87 21.867636363636365 22.0 10.5 33.0 6.0 37.0 88-89 21.662545454545455 22.0 6.0 33.0 6.0 37.0 90-91 21.323999999999998 22.0 6.0 33.0 6.0 37.0 92-93 21.30690909090909 22.0 6.0 33.0 6.0 37.0 94-95 21.225818181818184 22.0 6.0 33.0 6.0 37.0 96-97 20.920727272727273 22.0 6.0 33.0 6.0 37.0 98-99 20.606181818181817 22.0 6.0 33.0 6.0 37.0 100-101 20.124 18.5 6.0 33.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 8.838293650793648 2306 2 8.979563492063491 2306 3 8.714880952380952 2306 4 10.44007936507937 2306 5 10.258531746031746 2306 6 10.970238095238095 2306 7 9.880158730158737 2306 8 10.479761904761911 2306 9 10.922619047619047 2306 10-11 11.019940476190484 2306 12-13 11.453769841269843 2306 14-15 12.559523809523814 2306 16-17 12.639384920634917 2306 18-19 13.411706349206352 2306 20-21 12.461011904761904 2306 22-23 12.679067460317459 2306 24-25 12.380654761904765 2306 26-27 12.461904761904766 2306 28-29 13.4375 2306 30-31 14.142460317460323 2306 32-33 6.080456349206354 2306 34-35 12.323710317460318 2306 36-37 13.50575396825397 2306 38-39 14.079861111111114 2306 40-41 14.147023809523809 2306 42-43 7.710218253968247 2306 44-45 12.000099206349212 2306 46-47 13.851388888888884 2306 48-49 13.770337301587304 2306 50-51 12.821626984126986 2306 52-53 15.91359126984127 2306 54-55 9.646726190476187 2306 56-57 11.555456349206345 2306 58-59 12.478373015873018 2306 60-61 14.059027777777775 2306 62-63 13.545138888888886 2306 64-65 10.316369047619045 2306 66-67 11.839980158730153 2306 68-69 14.6375992063492 2306 70-71 11.020932539682537 2306 72-73 14.591170634920637 2306 74-75 13.510119047619042 2306 76-77 -4.758333333333333 2306 78-79 10.801785714285717 2306 80-81 9.469940476190473 2306 82-83 9.109722222222224 2306 84-85 13.34672619047619 2306 86-87 3.6400793650793624 2306 88-89 7.951587301587299 2306 90-91 12.530456349206347 2306 92-93 14.64107142857143 2306 94-95 14.400892857142857 2306 96-97 12.39990079365079 2306 98-99 4.552182539682537 2306 100-101 11.467757936507933 2307 1 8.838293650793648 2307 2 8.979563492063491 2307 3 8.714880952380952 2307 4 10.44007936507937 2307 5 10.258531746031746 2307 6 10.970238095238095 2307 7 9.880158730158737 2307 8 10.479761904761911 2307 9 10.922619047619047 2307 10-11 11.019940476190484 2307 12-13 11.453769841269843 2307 14-15 12.559523809523814 2307 16-17 12.639384920634917 2307 18-19 13.411706349206352 2307 20-21 12.461011904761904 2307 22-23 12.679067460317459 2307 24-25 13.880654761904765 2307 26-27 12.461904761904766 2307 28-29 11.9375 2307 30-31 14.142460317460323 2307 32-33 14.080456349206354 2307 34-35 13.823710317460318 2307 36-37 13.50575396825397 2307 38-39 14.079861111111114 2307 40-41 14.147023809523809 2307 42-43 14.210218253968247 2307 44-45 15.000099206349212 2307 46-47 15.351388888888884 2307 48-49 15.270337301587304 2307 50-51 12.821626984126986 2307 52-53 5.91359126984127 2307 54-55 9.646726190476187 2307 56-57 11.555456349206345 2307 58-59 6.978373015873018 2307 60-61 7.059027777777775 2307 62-63 10.045138888888886 2307 64-65 12.316369047619045 2307 66-67 9.839980158730153 2307 68-69 9.6375992063492 2307 70-71 6.020932539682537 2307 72-73 9.091170634920637 2307 74-75 9.510119047619042 2307 76-77 4.241666666666667 2307 78-79 -9.198214285714283 2307 80-81 -13.030059523809527 2307 82-83 6.109722222222224 2307 84-85 -17.65327380952381 2307 86-87 -16.359920634920638 2307 88-89 -3.548412698412701 2307 90-91 -11.969543650793653 2307 92-93 -16.35892857142857 2307 94-95 -16.599107142857143 2307 96-97 -16.60009920634921 2307 98-99 -16.447817460317463 2307 100-101 -7.532242063492067 2201 1 8.838293650793648 2201 2 8.979563492063491 2201 3 8.714880952380952 2201 4 10.44007936507937 2201 5 10.258531746031746 2201 6 10.970238095238095 2201 7 9.880158730158737 2201 8 6.4797619047619115 2201 9 6.9226190476190474 2201 10-11 9.019940476190484 2201 12-13 6.4537698412698425 2201 14-15 9.559523809523814 2201 16-17 11.139384920634917 2201 18-19 13.411706349206352 2201 20-21 8.961011904761904 2201 22-23 -1.3209325396825413 2201 24-25 3.880654761904765 2201 26-27 10.961904761904766 2201 28-29 3.4375 2201 30-31 11.142460317460323 2201 32-33 9.080456349206354 2201 34-35 5.823710317460318 2201 36-37 10.50575396825397 2201 38-39 11.079861111111114 2201 40-41 11.147023809523809 2201 42-43 -15.289781746031753 2201 44-45 8.000099206349212 2201 46-47 12.351388888888884 2201 48-49 7.270337301587304 2201 50-51 7.821626984126986 2201 52-53 12.91359126984127 2201 54-55 11.646726190476187 2201 56-57 -1.4445436507936549 2201 58-59 10.978373015873018 2201 60-61 11.059027777777775 2201 62-63 10.045138888888886 2201 64-65 8.316369047619045 2201 66-67 7.839980158730153 2201 68-69 13.1375992063492 2201 70-71 14.520932539682537 2201 72-73 2.091170634920637 2201 74-75 9.510119047619042 2201 76-77 3.741666666666667 2201 78-79 10.801785714285717 2201 80-81 9.469940476190473 2201 82-83 6.109722222222224 2201 84-85 9.34672619047619 2201 86-87 10.640079365079362 2201 88-89 7.951587301587299 2201 90-91 10.530456349206347 2201 92-93 10.64107142857143 2201 94-95 10.400892857142857 2201 96-97 7.39990079365079 2201 98-99 10.552182539682537 2201 100-101 11.467757936507933 2304 1 8.838293650793648 2304 2 8.979563492063491 2304 3 8.714880952380952 2304 4 10.44007936507937 2304 5 10.258531746031746 2304 6 10.970238095238095 2304 7 9.880158730158737 2304 8 10.479761904761911 2304 9 10.922619047619047 2304 10-11 11.019940476190484 2304 12-13 11.453769841269843 2304 14-15 9.559523809523814 2304 16-17 11.139384920634917 2304 18-19 13.411706349206352 2304 20-21 13.961011904761904 2304 22-23 12.679067460317459 2304 24-25 13.880654761904765 2304 26-27 13.961904761904766 2304 28-29 13.4375 2304 30-31 12.642460317460323 2304 32-33 12.580456349206354 2304 34-35 13.823710317460318 2304 36-37 13.50575396825397 2304 38-39 14.079861111111114 2304 40-41 14.147023809523809 2304 42-43 14.210218253968247 2304 44-45 15.000099206349212 2304 46-47 15.351388888888884 2304 48-49 15.270337301587304 2304 50-51 14.321626984126986 2304 52-53 14.41359126984127 2304 54-55 13.146726190476187 2304 56-57 14.555456349206345 2304 58-59 13.978373015873018 2304 60-61 14.059027777777775 2304 62-63 15.045138888888886 2304 64-65 15.316369047619045 2304 66-67 11.839980158730153 2304 68-69 11.6375992063492 2304 70-71 13.020932539682537 2304 72-73 13.091170634920637 2304 74-75 13.510119047619042 2304 76-77 13.741666666666667 2304 78-79 12.801785714285717 2304 80-81 13.469940476190473 2304 82-83 13.109722222222224 2304 84-85 13.34672619047619 2304 86-87 14.640079365079362 2304 88-89 12.951587301587299 2304 90-91 14.530456349206347 2304 92-93 14.64107142857143 2304 94-95 12.400892857142857 2304 96-97 14.39990079365079 2304 98-99 14.552182539682537 2304 100-101 15.467757936507933 2202 1 -3.1617063492063515 2202 2 -5.520436507936509 2202 3 -3.285119047619048 2202 4 -2.55992063492063 2202 5 -2.7414682539682538 2202 6 -5.029761904761905 2202 7 -1.1198412698412632 2202 8 -2.5202380952380885 2202 9 6.9226190476190474 2202 10-11 6.5199404761904844 2202 12-13 -5.7962301587301575 2202 14-15 -3.4404761904761862 2202 16-17 -12.360615079365083 2202 18-19 1.9117063492063515 2202 20-21 2.7110119047619037 2202 22-23 4.179067460317459 2202 24-25 -3.619345238095235 2202 26-27 -6.538095238095234 2202 28-29 -7.8125 2202 30-31 -4.857539682539677 2202 32-33 -4.169543650793646 2202 34-35 -0.42628968253968225 2202 36-37 2.00575396825397 2202 38-39 -6.920138888888886 2202 40-41 -13.602976190476191 2202 42-43 -3.0397817460317533 2202 44-45 -8.249900793650788 2202 46-47 -4.148611111111116 2202 48-49 9.270337301587304 2202 50-51 -0.17837301587301368 2202 52-53 -5.08640873015873 2202 54-55 -4.103273809523813 2202 56-57 -11.944543650793655 2202 58-59 -8.021626984126982 2202 60-61 -5.690972222222225 2202 62-63 -4.704861111111114 2202 64-65 -1.1836309523809554 2202 66-67 2.339980158730153 2202 68-69 -5.862400793650799 2202 70-71 8.520932539682537 2202 72-73 2.091170634920637 2202 74-75 -12.239880952380958 2202 76-77 -11.008333333333333 2202 78-79 -1.4482142857142826 2202 80-81 -3.030059523809527 2202 82-83 -15.640277777777776 2202 84-85 -13.15327380952381 2202 86-87 -6.609920634920638 2202 88-89 -4.548412698412701 2202 90-91 -4.9695436507936535 2202 92-93 0.3910714285714292 2202 94-95 -5.099107142857143 2202 96-97 -8.10009920634921 2202 98-99 -5.697817460317463 2202 100-101 -8.032242063492067 2305 1 8.838293650793648 2305 2 8.979563492063491 2305 3 8.714880952380952 2305 4 10.44007936507937 2305 5 10.258531746031746 2305 6 10.970238095238095 2305 7 9.880158730158737 2305 8 10.479761904761911 2305 9 10.922619047619047 2305 10-11 11.019940476190484 2305 12-13 11.453769841269843 2305 14-15 12.559523809523814 2305 16-17 11.139384920634917 2305 18-19 10.411706349206352 2305 20-21 10.961011904761904 2305 22-23 12.679067460317459 2305 24-25 13.880654761904765 2305 26-27 12.461904761904766 2305 28-29 13.4375 2305 30-31 14.142460317460323 2305 32-33 12.580456349206354 2305 34-35 13.823710317460318 2305 36-37 10.50575396825397 2305 38-39 14.079861111111114 2305 40-41 12.647023809523809 2305 42-43 14.210218253968247 2305 44-45 15.000099206349212 2305 46-47 15.351388888888884 2305 48-49 13.770337301587304 2305 50-51 14.321626984126986 2305 52-53 14.41359126984127 2305 54-55 14.646726190476187 2305 56-57 14.555456349206345 2305 58-59 13.978373015873018 2305 60-61 14.059027777777775 2305 62-63 15.045138888888886 2305 64-65 15.316369047619045 2305 66-67 14.839980158730153 2305 68-69 13.1375992063492 2305 70-71 16.020932539682537 2305 72-73 13.091170634920637 2305 74-75 16.510119047619042 2305 76-77 16.741666666666667 2305 78-79 14.301785714285717 2305 80-81 13.469940476190473 2305 82-83 13.109722222222224 2305 84-85 13.34672619047619 2305 86-87 14.640079365079362 2305 88-89 14.951587301587299 2305 90-91 14.530456349206347 2305 92-93 14.64107142857143 2305 94-95 14.400892857142857 2305 96-97 14.39990079365079 2305 98-99 12.552182539682537 2305 100-101 11.467757936507933 2203 1 1.5882936507936485 2203 2 5.979563492063491 2203 3 5.714880952380952 2203 4 -1.0599206349206298 2203 5 -2.2414682539682538 2203 6 -0.5297619047619051 2203 7 -4.369841269841263 2203 8 -6.0202380952380885 2203 9 -4.5773809523809526 2203 10-11 -2.1050595238095156 2203 12-13 0.9537698412698425 2203 14-15 -2.1904761904761862 2203 16-17 -2.8606150793650826 2203 18-19 -4.4632936507936485 2203 20-21 -1.2889880952380963 2203 22-23 -10.570932539682541 2203 24-25 -2.369345238095235 2203 26-27 -2.5380952380952344 2203 28-29 -4.3125 2203 30-31 -2.3575396825396773 2203 32-33 2.8304563492063544 2203 34-35 -0.6762896825396822 2203 36-37 -0.6192460317460302 2203 38-39 -1.4201388888888857 2203 40-41 -2.227976190476191 2203 42-43 1.2102182539682467 2203 44-45 2.375099206349212 2203 46-47 -2.6486111111111157 2203 48-49 -2.729662698412696 2203 50-51 -1.4283730158730137 2203 52-53 3.1635912698412696 2203 54-55 2.521726190476187 2203 56-57 2.055456349206345 2203 58-59 -0.27162698412698205 2203 60-61 0.18402777777777501 2203 62-63 2.4201388888888857 2203 64-65 1.8163690476190446 2203 66-67 2.464980158730153 2203 68-69 -0.11240079365079936 2203 70-71 -3.604067460317463 2203 72-73 3.591170634920637 2203 74-75 3.6351190476190425 2203 76-77 5.241666666666667 2203 78-79 0.9267857142857174 2203 80-81 4.469940476190473 2203 82-83 0.9847222222222243 2203 84-85 2.59672619047619 2203 86-87 4.890079365079362 2203 88-89 1.701587301587299 2203 90-91 3.9054563492063465 2203 92-93 2.391071428571429 2203 94-95 -3.099107142857143 2203 96-97 -1.3500992063492099 2203 98-99 2.427182539682537 2203 100-101 1.4677579365079332 1103 1 5.8382936507936485 1103 2 0.479563492063491 1103 3 5.714880952380952 1103 4 -5.05992063492063 1103 5 5.258531746031746 1103 6 5.970238095238095 1103 7 -1.1198412698412632 1103 8 -0.5202380952380885 1103 9 -2.0773809523809526 1103 10-11 -3.2300595238095156 1103 12-13 -2.7962301587301575 1103 14-15 2.3095238095238138 1103 16-17 2.6393849206349174 1103 18-19 6.9117063492063515 1103 20-21 7.461011904761904 1103 22-23 -4.820932539682541 1103 24-25 -2.369345238095235 1103 26-27 0.7119047619047656 1103 28-29 4.1875 1103 30-31 0.8924603174603227 1103 32-33 2.0804563492063544 1103 34-35 4.573710317460318 1103 36-37 3.25575396825397 1103 38-39 5.079861111111114 1103 40-41 5.147023809523809 1103 42-43 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84-85 0.0967261904761898 1215 86-87 1.3900793650793624 1215 88-89 3.451587301587299 1215 90-91 1.2804563492063465 1215 92-93 7.391071428571429 1215 94-95 12.400892857142857 1215 96-97 9.39990079365079 1215 98-99 1.302182539682537 1215 100-101 4.467757936507933 1213 1 1.8382936507936485 1213 2 1.7795634920634917 1213 3 -0.08511904761904887 1213 4 -5.3599206349206305 1213 5 -5.5414682539682545 1213 6 2.970238095238095 1213 7 0.2801587301587354 1213 8 -0.3202380952380892 1213 9 3.9226190476190474 1213 10-11 4.219940476190484 1213 12-13 2.7537698412698433 1213 14-15 2.0595238095238138 1213 16-17 2.1393849206349174 1213 18-19 -0.08829365079364848 1213 20-21 1.3610119047619023 1213 22-23 -1.3209325396825413 1213 24-25 1.2806547619047635 1213 26-27 0.16190476190476488 1213 28-29 4.4375 1213 30-31 1.8424603174603256 1213 32-33 -2.2195436507936463 1213 34-35 -2.576289682539681 1213 36-37 1.1057539682539712 1213 38-39 -0.42013888888888573 1213 40-41 0.04702380952380736 1213 42-43 4.510218253968247 1213 44-45 3.7000992063492113 1213 46-47 5.751388888888886 1213 48-49 4.270337301587304 1213 50-51 1.721626984126985 1213 52-53 3.7135912698412703 1213 54-55 3.446726190476184 1213 56-57 1.8554563492063458 1213 58-59 -0.12162698412698347 1213 60-61 1.559027777777775 1213 62-63 3.5451388888888857 1213 64-65 0.6163690476190418 1213 66-67 0.23998015873015177 1213 68-69 2.537599206349199 1213 70-71 8.22093253968254 1213 72-73 1.3911706349206376 1213 74-75 3.410119047619041 1213 76-77 4.441666666666666 1213 78-79 3.701785714285716 1213 80-81 6.369940476190472 1213 82-83 4.809722222222224 1213 84-85 3.84672619047619 1213 86-87 -0.9599206349206391 1213 88-89 0.3515873015872977 1213 90-91 3.930456349206345 1213 92-93 5.041071428571428 1213 94-95 5.10089285714286 1213 96-97 6.099900793650793 1213 98-99 3.2521825396825363 1213 100-101 2.467757936507933 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 12.0 8 27.0 9 39.0 10 47.0 11 74.0 12 73.0 13 45.0 14 66.0 15 41.0 16 54.0 17 32.0 18 37.0 19 36.0 20 35.0 21 33.0 22 28.0 23 30.0 24 25.0 25 26.0 26 30.0 27 23.0 28 31.0 29 27.0 30 41.0 31 36.0 32 26.0 33 43.0 34 51.0 35 57.0 36 81.0 37 105.0 38 61.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.96363636363637 26.036363636363635 12.509090909090908 23.49090909090909 2 14.909090909090908 27.27272727272727 38.32727272727273 19.490909090909092 3 18.327272727272728 28.50909090909091 32.07272727272728 21.09090909090909 4 14.472727272727273 21.454545454545453 37.74545454545454 26.327272727272728 5 13.090909090909092 40.43636363636364 33.6 12.872727272727271 6 26.763636363636362 42.472727272727276 17.01818181818182 13.745454545454546 7 23.563636363636363 36.29090909090909 22.327272727272724 17.81818181818182 8 21.6 42.32727272727273 19.490909090909092 16.581818181818182 9 23.49090909090909 20.363636363636363 20.072727272727274 36.07272727272727 10-11 24.0 31.381818181818183 26.836363636363636 17.78181818181818 12-13 22.03636363636364 28.61818181818182 27.636363636363637 21.709090909090907 14-15 21.2 31.345454545454544 22.145454545454545 25.30909090909091 16-17 20.254545454545454 35.81818181818181 25.236363636363635 18.69090909090909 18-19 21.6 33.74545454545454 27.418181818181818 17.236363636363635 20-21 22.727272727272727 32.32727272727273 26.654545454545453 18.29090909090909 22-23 20.363636363636363 33.6 26.436363636363637 19.6 24-25 21.527272727272727 33.30909090909091 27.01818181818182 18.145454545454545 26-27 20.581818181818182 36.0 24.90909090909091 18.509090909090908 28-29 20.654545454545453 34.61818181818182 26.327272727272728 18.4 30-31 19.78181818181818 35.163636363636364 26.581818181818186 18.472727272727273 32-33 21.09090909090909 33.70909090909091 25.490909090909092 19.70909090909091 34-35 21.381818181818183 34.36363636363636 24.509090909090908 19.745454545454546 36-37 20.909090909090907 34.07272727272727 27.16363636363636 17.854545454545452 38-39 20.327272727272728 34.32727272727273 25.745454545454542 19.6 40-41 21.78181818181818 35.090909090909086 25.345454545454544 17.78181818181818 42-43 20.29090909090909 34.47272727272727 25.2 20.036363636363635 44-45 22.03636363636364 33.6 25.09090909090909 19.272727272727273 46-47 19.818181818181817 35.38181818181818 26.400000000000002 18.4 48-49 19.963636363636365 33.30909090909091 26.981818181818184 19.745454545454546 50-51 20.327272727272728 31.89090909090909 27.200000000000003 20.581818181818182 52-53 19.345454545454547 33.054545454545455 27.92727272727273 19.672727272727272 54-55 18.509090909090908 33.70909090909091 26.90909090909091 20.872727272727275 56-57 20.581818181818182 31.963636363636365 28.654545454545456 18.8 58-59 18.072727272727274 33.2 28.18181818181818 20.545454545454543 60-61 19.418181818181818 32.981818181818184 27.01818181818182 20.581818181818182 62-63 19.30909090909091 31.418181818181818 27.345454545454544 21.92727272727273 64-65 20.036363636363635 30.654545454545456 28.03636363636364 21.272727272727273 66-67 20.581818181818182 32.14545454545454 26.036363636363635 21.236363636363638 68-69 20.0 32.32727272727273 26.50909090909091 21.163636363636364 70-71 19.38181818181818 32.654545454545456 27.345454545454544 20.618181818181817 72-73 19.418181818181818 32.32727272727273 27.6 20.654545454545453 74-75 19.70909090909091 30.94545454545455 27.16363636363636 22.181818181818183 76-77 19.236363636363638 31.563636363636366 28.000000000000004 21.2 78-79 20.036363636363635 32.690909090909095 26.181818181818183 21.09090909090909 80-81 19.927272727272726 32.14545454545454 25.96363636363636 21.96363636363636 82-83 19.527272727272727 33.018181818181816 25.272727272727273 22.181818181818183 84-85 19.854545454545455 32.21818181818182 26.254545454545454 21.672727272727276 86-87 19.2 34.47272727272727 25.89090909090909 20.436363636363637 88-89 20.254545454545454 30.94545454545455 26.327272727272728 22.472727272727273 90-91 21.381818181818183 31.89090909090909 25.381818181818183 21.345454545454544 92-93 20.145454545454545 32.36363636363636 25.89090909090909 21.6 94-95 20.872727272727275 31.672727272727276 25.636363636363633 21.818181818181817 96-97 21.96363636363636 32.03636363636363 24.981818181818184 21.01818181818182 98-99 21.236363636363638 32.07272727272728 25.381818181818183 21.30909090909091 100-101 21.2 31.6 25.381818181818183 21.818181818181817 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 1.0 14 1.0 15 0.5 16 1.5 17 2.5 18 2.0 19 3.0 20 4.5 21 5.0 22 8.0 23 8.5 24 12.5 25 17.5 26 19.5 27 26.0 28 27.0 29 28.0 30 32.5 31 37.5 32 40.5 33 40.5 34 45.0 35 45.0 36 42.5 37 54.0 38 62.5 39 57.0 40 49.0 41 48.0 42 49.0 43 48.0 44 48.5 45 50.0 46 47.5 47 43.0 48 42.0 49 39.5 50 30.0 51 25.0 52 26.5 53 25.5 54 23.5 55 22.5 56 22.0 57 15.5 58 12.5 59 10.0 60 8.5 61 7.5 62 7.5 63 9.5 64 10.0 65 8.0 66 6.0 67 4.0 68 1.5 69 1.0 70 1.0 71 1.5 72 0.5 73 1.5 74 2.0 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1375.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-11 0.0 0.0 0.0 12-13 0.0 0.0 0.0 14-15 0.0 0.0 0.0 16-17 0.0 0.0 0.0 18-19 0.0 0.0 0.0 20-21 0.0 0.0 0.0 22-23 0.0 0.0 0.0 24-25 0.0 0.0 0.0 26-27 0.0 0.0 0.0 28-29 0.0 0.0 0.07272727272727272 30-31 0.0 0.0 0.07272727272727272 32-33 0.0 0.0 0.14545454545454545 34-35 0.0 0.0 0.18181818181818182 36-37 0.0 0.0 0.3636363636363636 38-39 0.0 0.0 0.6909090909090909 40-41 0.0 0.0 1.0909090909090908 42-43 0.0 0.0 1.4181818181818182 44-45 0.0 0.0 1.7454545454545456 46-47 0.0 0.0 2.327272727272727 48-49 0.0 0.0 3.381818181818182 50-51 0.0 0.0 4.109090909090909 52-53 0.0 0.0 4.618181818181818 54-55 0.0 0.0 5.454545454545455 56-57 0.0 0.0 6.0 58-59 0.0 0.0 6.472727272727273 60-61 0.0 0.0 7.054545454545455 62-63 0.0 0.0 7.527272727272727 64-65 0.0 0.0 8.0 66-67 0.0 0.0 8.545454545454547 68-69 0.0 0.0 8.836363636363636 70-71 0.0 0.0 9.309090909090909 72-73 0.0 0.0 9.818181818181818 74-75 0.0 0.0 10.10909090909091 76-77 0.0 0.0 10.763636363636364 78-79 0.0 0.0 11.418181818181818 80-81 0.0 0.0 11.854545454545455 82-83 0.0 0.0 12.4 84-85 0.0 0.0 12.763636363636364 86-87 0.0 0.0 13.01818181818182 88-89 0.0 0.0 13.381818181818183 >>END_MODULE >>Kmer Content pass >>END_MODULE