##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename G140_A6_L52_TAAGGCGA-GTAAGGAG_L006_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1375 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.965090909090907 33.0 33.0 33.0 27.0 33.0 2 32.19418181818182 33.0 33.0 33.0 33.0 33.0 3 32.151272727272726 33.0 33.0 33.0 27.0 33.0 4 35.966545454545454 37.0 37.0 37.0 33.0 37.0 5 35.584 37.0 37.0 37.0 33.0 37.0 6 35.492363636363635 37.0 37.0 37.0 33.0 37.0 7 35.47709090909091 37.0 37.0 37.0 33.0 37.0 8 35.56363636363636 37.0 37.0 37.0 33.0 37.0 9 35.71054545454545 37.0 37.0 37.0 33.0 37.0 10-11 35.49527272727273 37.0 37.0 37.0 33.0 37.0 12-13 35.47890909090909 37.0 37.0 37.0 33.0 37.0 14-15 36.71090909090909 37.0 37.0 40.0 33.0 40.0 16-17 36.55272727272727 37.0 37.0 40.0 33.0 40.0 18-19 36.62254545454546 37.0 37.0 40.0 33.0 40.0 20-21 36.46763636363636 37.0 37.0 40.0 33.0 40.0 22-23 36.31309090909091 37.0 37.0 40.0 33.0 40.0 24-25 36.29890909090909 37.0 37.0 40.0 33.0 40.0 26-27 36.104727272727274 37.0 37.0 40.0 33.0 40.0 28-29 35.78 37.0 35.0 40.0 27.0 40.0 30-31 35.51345454545455 37.0 33.0 40.0 27.0 40.0 32-33 35.406909090909096 37.0 33.0 40.0 27.0 40.0 34-35 35.23709090909091 37.0 33.0 40.0 27.0 40.0 36-37 34.865818181818184 37.0 33.0 40.0 27.0 40.0 38-39 34.70727272727272 37.0 33.0 40.0 27.0 40.0 40-41 34.30181818181818 37.0 33.0 40.0 22.0 40.0 42-43 34.25054545454546 37.0 33.0 40.0 24.5 40.0 44-45 33.900363636363636 37.0 33.0 40.0 22.0 40.0 46-47 33.39381818181818 37.0 33.0 38.5 22.0 40.0 48-49 33.41345454545454 37.0 33.0 37.0 22.0 40.0 50-51 33.475272727272724 37.0 33.0 37.0 22.0 40.0 52-53 33.28909090909091 37.0 33.0 37.0 22.0 40.0 54-55 32.83309090909091 37.0 33.0 37.0 22.0 40.0 56-57 32.49345454545455 37.0 30.0 37.0 18.5 40.0 58-59 32.23709090909091 37.0 30.0 37.0 15.0 40.0 60-61 31.65745454545455 35.0 27.0 37.0 15.0 40.0 62-63 31.29381818181818 33.0 27.0 37.0 15.0 40.0 64-65 31.054545454545455 33.0 27.0 37.0 15.0 40.0 66-67 30.469454545454546 33.0 27.0 37.0 15.0 40.0 68-69 30.338181818181816 33.0 27.0 37.0 15.0 38.5 70-71 29.963272727272727 33.0 27.0 37.0 15.0 37.0 72-73 29.373454545454546 33.0 27.0 37.0 15.0 37.0 74-75 29.055636363636363 33.0 27.0 37.0 15.0 37.0 76-77 27.609454545454547 33.0 22.0 35.0 6.0 37.0 78-79 28.559636363636365 33.0 22.0 37.0 15.0 37.0 80-81 28.254545454545454 33.0 22.0 37.0 6.0 37.0 82-83 27.968363636363634 33.0 22.0 37.0 6.0 37.0 84-85 27.726181818181818 33.0 22.0 37.0 6.0 37.0 86-87 27.497454545454545 33.0 22.0 37.0 6.0 37.0 88-89 27.20218181818182 33.0 22.0 37.0 6.0 37.0 90-91 26.569454545454548 33.0 15.0 37.0 6.0 37.0 92-93 26.391636363636366 33.0 15.0 33.0 6.0 37.0 94-95 26.401454545454545 33.0 15.0 33.0 6.0 37.0 96-97 26.14181818181818 33.0 15.0 33.0 6.0 37.0 98-99 25.862181818181817 33.0 15.0 33.0 6.0 37.0 100-101 25.33490909090909 30.0 15.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 1.3412698412698383 2306 2 0.8214285714285694 2306 3 0.577380952380949 2306 4 1.1702380952380977 2306 5 1.8392857142857224 2306 6 1.8313492063492163 2306 7 1.7380952380952408 2306 8 1.1329365079365203 2306 9 0.9821428571428541 2306 10-11 1.5376984126984183 2306 12-13 2.2537698412698504 2306 14-15 3.294841269841271 2306 16-17 3.3348214285714306 2306 18-19 3.5115079365079396 2306 20-21 2.1151785714285722 2306 22-23 2.475099206349199 2306 24-25 2.176884920634926 2306 26-27 3.6404761904761926 2306 28-29 3.542162698412696 2306 30-31 2.4353174603174566 2306 32-33 2.489087301587304 2306 34-35 3.176686507936516 2306 36-37 5.442857142857143 2306 38-39 4.650099206349218 2306 40-41 4.843253968253968 2306 42-43 3.854662698412703 2306 44-45 2.9805555555555543 2306 46-47 4.9164682539682545 2306 48-49 6.024007936507928 2306 50-51 6.399404761904762 2306 52-53 5.951587301587303 2306 54-55 5.94603174603175 2306 56-57 6.620039682539677 2306 58-59 6.747321428571432 2306 60-61 7.174007936507941 2306 62-63 8.05734126984127 2306 64-65 7.6815476190476275 2306 66-67 5.315575396825402 2306 68-69 8.34652777777778 2306 70-71 7.290773809523813 2306 72-73 5.8765873015873105 2306 74-75 6.570634920634916 2306 76-77 11.111111111111107 2306 78-79 7.8533730158730215 2306 80-81 6.72113095238095 2306 82-83 7.575793650793646 2306 84-85 7.153769841269838 2306 86-87 7.573710317460318 2306 88-89 8.332837301587304 2306 90-91 7.053968253968254 2306 92-93 6.6887896825396815 2306 94-95 8.711309523809526 2306 96-97 10.09990079365079 2306 98-99 7.959523809523802 2306 100-101 8.290972222222226 2307 1 1.3412698412698383 2307 2 0.8214285714285694 2307 3 0.577380952380949 2307 4 1.1702380952380977 2307 5 1.8392857142857224 2307 6 1.8313492063492163 2307 7 1.7380952380952408 2307 8 1.1329365079365203 2307 9 0.9821428571428541 2307 10-11 1.5376984126984183 2307 12-13 2.2537698412698504 2307 14-15 0.294841269841271 2307 16-17 3.3348214285714306 2307 18-19 3.5115079365079396 2307 20-21 3.6151785714285722 2307 22-23 -1.0249007936508008 2307 24-25 0.6768849206349259 2307 26-27 3.6404761904761926 2307 28-29 3.542162698412696 2307 30-31 3.9353174603174566 2307 32-33 3.989087301587304 2307 34-35 1.676686507936516 2307 36-37 2.442857142857143 2307 38-39 -0.3499007936507823 2307 40-41 1.3432539682539684 2307 42-43 2.354662698412703 2307 44-45 2.9805555555555543 2307 46-47 3.4164682539682545 2307 48-49 1.0240079365079282 2307 50-51 -0.600595238095238 2307 52-53 0.9515873015873026 2307 54-55 2.9460317460317498 2307 56-57 3.6200396825396766 2307 58-59 1.747321428571432 2307 60-61 2.174007936507941 2307 62-63 1.0573412698412703 2307 64-65 2.6815476190476275 2307 66-67 3.315575396825402 2307 68-69 5.34652777777778 2307 70-71 1.790773809523813 2307 72-73 1.8765873015873105 2307 74-75 4.570634920634916 2307 76-77 4.111111111111107 2307 78-79 2.3533730158730215 2307 80-81 2.7211309523809497 2307 82-83 5.575793650793646 2307 84-85 5.153769841269838 2307 86-87 5.573710317460318 2307 88-89 8.332837301587304 2307 90-91 9.053968253968254 2307 92-93 6.6887896825396815 2307 94-95 8.711309523809526 2307 96-97 10.09990079365079 2307 98-99 7.959523809523802 2307 100-101 6.290972222222226 2201 1 1.3412698412698383 2201 2 0.8214285714285694 2201 3 0.577380952380949 2201 4 1.1702380952380977 2201 5 -8.160714285714278 2201 6 1.8313492063492163 2201 7 1.7380952380952408 2201 8 1.1329365079365203 2201 9 0.9821428571428541 2201 10-11 1.5376984126984183 2201 12-13 -2.7462301587301496 2201 14-15 -10.705158730158729 2201 16-17 -6.665178571428569 2201 18-19 -6.48849206349206 2201 20-21 -3.3848214285714278 2201 22-23 -9.0249007936508 2201 24-25 -12.323115079365074 2201 26-27 -16.859523809523807 2201 28-29 -6.457837301587304 2201 30-31 -6.0646825396825434 2201 32-33 0.9890873015873041 2201 34-35 -2.323313492063484 2201 36-37 -15.057142857142857 2201 38-39 1.6500992063492177 2201 40-41 -17.15674603174603 2201 42-43 0.35466269841270304 2201 44-45 -14.519444444444446 2201 46-47 -9.083531746031746 2201 48-49 -3.975992063492072 2201 50-51 -6.600595238095238 2201 52-53 -7.048412698412697 2201 54-55 -1.0539682539682502 2201 56-57 -16.879960317460323 2201 58-59 1.747321428571432 2201 60-61 -3.325992063492059 2201 62-63 -7.44265873015873 2201 64-65 -4.8184523809523725 2201 66-67 -1.6844246031745982 2201 68-69 -4.65347222222222 2201 70-71 -4.209226190476187 2201 72-73 -4.1234126984126895 2201 74-75 -13.929365079365084 2201 76-77 -10.388888888888893 2201 78-79 2.3533730158730215 2201 80-81 -9.27886904761905 2201 82-83 -18.924206349206354 2201 84-85 -23.84623015873016 2201 86-87 -18.926289682539682 2201 88-89 -22.667162698412696 2201 90-91 -6.946031746031746 2201 92-93 -1.3112103174603185 2201 94-95 -7.2886904761904745 2201 96-97 -20.90009920634921 2201 98-99 -13.040476190476198 2201 100-101 -16.209027777777774 2304 1 1.3412698412698383 2304 2 0.8214285714285694 2304 3 0.577380952380949 2304 4 1.1702380952380977 2304 5 1.8392857142857224 2304 6 1.8313492063492163 2304 7 1.7380952380952408 2304 8 1.1329365079365203 2304 9 0.9821428571428541 2304 10-11 1.5376984126984183 2304 12-13 2.2537698412698504 2304 14-15 3.294841269841271 2304 16-17 3.3348214285714306 2304 18-19 3.5115079365079396 2304 20-21 3.6151785714285722 2304 22-23 3.975099206349199 2304 24-25 3.676884920634926 2304 26-27 3.6404761904761926 2304 28-29 3.542162698412696 2304 30-31 3.9353174603174566 2304 32-33 2.489087301587304 2304 34-35 3.176686507936516 2304 36-37 5.442857142857143 2304 38-39 4.650099206349218 2304 40-41 6.343253968253968 2304 42-43 2.354662698412703 2304 44-45 5.980555555555554 2304 46-47 6.4164682539682545 2304 48-49 6.024007936507928 2304 50-51 6.399404761904762 2304 52-53 5.951587301587303 2304 54-55 5.94603174603175 2304 56-57 6.620039682539677 2304 58-59 5.247321428571432 2304 60-61 7.174007936507941 2304 62-63 8.05734126984127 2304 64-65 7.6815476190476275 2304 66-67 8.315575396825402 2304 68-69 6.84652777777778 2304 70-71 5.790773809523813 2304 72-73 5.8765873015873105 2304 74-75 6.570634920634916 2304 76-77 6.111111111111107 2304 78-79 6.3533730158730215 2304 80-81 6.72113095238095 2304 82-83 7.575793650793646 2304 84-85 7.153769841269838 2304 86-87 7.573710317460318 2304 88-89 8.332837301587304 2304 90-91 9.053968253968254 2304 92-93 6.6887896825396815 2304 94-95 8.711309523809526 2304 96-97 10.09990079365079 2304 98-99 9.959523809523802 2304 100-101 8.290972222222226 2202 1 1.3412698412698383 2202 2 -2.1785714285714306 2202 3 0.577380952380949 2202 4 -0.8297619047619023 2202 5 1.8392857142857224 2202 6 -9.168650793650784 2202 7 -9.26190476190476 2202 8 -9.86706349206348 2202 9 -3.017857142857146 2202 10-11 -0.9623015873015817 2202 12-13 -5.24623015873015 2202 14-15 -0.705158730158729 2202 16-17 -8.66517857142857 2202 18-19 -2.9884920634920604 2202 20-21 -2.1348214285714278 2202 22-23 -11.0249007936508 2202 24-25 -0.3231150793650741 2202 26-27 -5.859523809523807 2202 28-29 -15.457837301587304 2202 30-31 -7.3146825396825434 2202 32-33 -16.510912698412696 2202 34-35 -11.823313492063484 2202 36-37 -6.557142857142857 2202 38-39 -6.849900793650782 2202 40-41 -13.156746031746032 2202 42-43 -6.645337301587297 2202 44-45 -4.019444444444446 2202 46-47 -19.083531746031746 2202 48-49 -4.475992063492072 2202 50-51 -3.100595238095238 2202 52-53 -14.048412698412697 2202 54-55 -21.30396825396825 2202 56-57 -8.129960317460323 2202 58-59 -7.752678571428568 2202 60-61 -16.82599206349206 2202 62-63 -18.44265873015873 2202 64-65 -20.068452380952372 2202 66-67 -18.934424603174598 2202 68-69 -13.15347222222222 2202 70-71 -20.709226190476187 2202 72-73 -13.62341269841269 2202 74-75 -19.179365079365084 2202 76-77 -7.388888888888893 2202 78-79 -4.6466269841269785 2202 80-81 2.2211309523809497 2202 82-83 2.0757936507936456 2202 84-85 -11.846230158730162 2202 86-87 -2.1762896825396822 2202 88-89 -10.667162698412696 2202 90-91 -15.696031746031746 2202 92-93 -10.811210317460318 2202 94-95 -9.038690476190474 2202 96-97 -20.90009920634921 2202 98-99 -16.5404761904762 2202 100-101 -13.959027777777774 2305 1 1.3412698412698383 2305 2 0.8214285714285694 2305 3 0.577380952380949 2305 4 1.1702380952380977 2305 5 1.8392857142857224 2305 6 1.8313492063492163 2305 7 1.7380952380952408 2305 8 1.1329365079365203 2305 9 0.9821428571428541 2305 10-11 1.5376984126984183 2305 12-13 2.2537698412698504 2305 14-15 1.794841269841271 2305 16-17 3.3348214285714306 2305 18-19 3.5115079365079396 2305 20-21 2.1151785714285722 2305 22-23 2.475099206349199 2305 24-25 3.676884920634926 2305 26-27 3.6404761904761926 2305 28-29 3.542162698412696 2305 30-31 3.9353174603174566 2305 32-33 3.989087301587304 2305 34-35 4.676686507936516 2305 36-37 5.442857142857143 2305 38-39 4.650099206349218 2305 40-41 4.843253968253968 2305 42-43 5.354662698412703 2305 44-45 5.980555555555554 2305 46-47 6.4164682539682545 2305 48-49 6.024007936507928 2305 50-51 6.399404761904762 2305 52-53 5.951587301587303 2305 54-55 5.94603174603175 2305 56-57 5.120039682539677 2305 58-59 6.747321428571432 2305 60-61 7.174007936507941 2305 62-63 8.05734126984127 2305 64-65 7.6815476190476275 2305 66-67 8.315575396825402 2305 68-69 8.34652777777778 2305 70-71 3.790773809523813 2305 72-73 3.8765873015873105 2305 74-75 6.570634920634916 2305 76-77 8.111111111111107 2305 78-79 6.3533730158730215 2305 80-81 6.72113095238095 2305 82-83 7.575793650793646 2305 84-85 7.153769841269838 2305 86-87 7.573710317460318 2305 88-89 8.332837301587304 2305 90-91 9.053968253968254 2305 92-93 8.688789682539682 2305 94-95 6.7113095238095255 2305 96-97 8.09990079365079 2305 98-99 9.959523809523802 2305 100-101 10.290972222222226 2203 1 -0.15873015873016172 2203 2 0.8214285714285694 2203 3 0.577380952380949 2203 4 1.1702380952380977 2203 5 -1.6607142857142776 2203 6 -0.6686507936507837 2203 7 0.7380952380952408 2203 8 0.13293650793652034 2203 9 -0.017857142857145902 2203 10-11 -1.8373015873015817 2203 12-13 0.25376984126985036 2203 14-15 1.044841269841271 2203 16-17 -4.790178571428569 2203 18-19 -3.2384920634920604 2203 20-21 1.7401785714285722 2203 22-23 -2.524900793650801 2203 24-25 -7.698115079365074 2203 26-27 -5.359523809523807 2203 28-29 -0.7078373015873041 2203 30-31 -1.4396825396825434 2203 32-33 2.114087301587304 2203 34-35 0.17668650793651608 2203 36-37 2.567857142857143 2203 38-39 0.025099206349217695 2203 40-41 1.5932539682539684 2203 42-43 1.354662698412703 2203 44-45 1.1055555555555543 2203 46-47 2.0414682539682545 2203 48-49 1.7740079365079282 2203 50-51 0.899404761904762 2203 52-53 1.3265873015873026 2203 54-55 -0.5539682539682502 2203 56-57 -1.5049603174603234 2203 58-59 2.247321428571432 2203 60-61 0.674007936507941 2203 62-63 1.3073412698412703 2203 64-65 -5.5684523809523725 2203 66-67 -3.809424603174598 2203 68-69 -6.40347222222222 2203 70-71 -12.709226190476187 2203 72-73 -8.24841269841269 2203 74-75 -0.6793650793650841 2203 76-77 0.23611111111110716 2203 78-79 -2.8966269841269785 2203 80-81 -12.15386904761905 2203 82-83 -7.424206349206354 2203 84-85 -3.5962301587301617 2203 86-87 -11.676289682539682 2203 88-89 -11.292162698412696 2203 90-91 -14.196031746031746 2203 92-93 -11.936210317460318 2203 94-95 -8.413690476190474 2203 96-97 -7.150099206349211 2203 98-99 -8.290476190476198 2203 100-101 1.0409722222222264 1103 1 1.3412698412698383 1103 2 -2.1785714285714306 1103 3 -7.922619047619051 1103 4 -5.829761904761902 1103 5 1.8392857142857224 1103 6 -7.668650793650784 1103 7 -3.261904761904759 1103 8 -0.8670634920634797 1103 9 -1.017857142857146 1103 10-11 -5.962301587301582 1103 12-13 -0.7462301587301496 1103 14-15 -0.705158730158729 1103 16-17 -0.6651785714285694 1103 18-19 -4.48849206349206 1103 20-21 -2.8848214285714278 1103 22-23 -1.5249007936508008 1103 24-25 -3.823115079365074 1103 26-27 -1.3595238095238074 1103 28-29 -0.45783730158730407 1103 30-31 -7.8146825396825434 1103 32-33 -2.510912698412696 1103 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1.9711309523809497 1215 82-83 5.575793650793646 1215 84-85 5.153769841269838 1215 86-87 -0.9262896825396822 1215 88-89 3.832837301587304 1215 90-91 -6.446031746031746 1215 92-93 -4.3112103174603185 1215 94-95 -1.5386904761904745 1215 96-97 -5.150099206349211 1215 98-99 -2.0404761904761983 1215 100-101 -3.9590277777777736 1213 1 1.3412698412698383 1213 2 0.8214285714285694 1213 3 0.577380952380949 1213 4 0.3702380952381006 1213 5 1.8392857142857224 1213 6 1.8313492063492163 1213 7 1.7380952380952408 1213 8 1.1329365079365203 1213 9 0.9821428571428541 1213 10-11 1.5376984126984183 1213 12-13 1.8537698412698518 1213 14-15 -0.3051587301587233 1213 16-17 -2.6651785714285694 1213 18-19 0.7115079365079424 1213 20-21 1.315178571428575 1213 22-23 -0.22490079365080362 1213 24-25 -1.2231150793650727 1213 26-27 0.8404761904761955 1213 28-29 -1.457837301587304 1213 30-31 -0.46468253968254203 1213 32-33 0.48908730158730407 1213 34-35 0.8766865079365189 1213 36-37 1.2428571428571402 1213 38-39 -1.249900793650788 1213 40-41 0.8432539682539684 1213 42-43 1.354662698412703 1213 44-45 1.7805555555555515 1213 46-47 1.016468253968256 1213 48-49 -2.075992063492073 1213 50-51 -4.400595238095235 1213 52-53 -1.6484126984126988 1213 54-55 -0.8539682539682474 1213 56-57 -1.3799603174603234 1213 58-59 -0.6526785714285666 1213 60-61 -1.5259920634920583 1213 62-63 -1.8426587301587283 1213 64-65 -3.8184523809523725 1213 66-67 -6.484424603174599 1213 68-69 -3.0534722222222186 1213 70-71 -1.5092261904761877 1213 72-73 -1.9234126984126867 1213 74-75 -2.5293650793650855 1213 76-77 1.6111111111111072 1213 78-79 -3.74662698412698 1213 80-81 -1.9788690476190496 1213 82-83 -0.12420634920635365 1213 84-85 -6.846230158730162 1213 86-87 -7.326289682539681 1213 88-89 -4.167162698412696 1213 90-91 -2.6460317460317455 1213 92-93 -2.711210317460317 1213 94-95 -0.2886904761904745 1213 96-97 0.499900793650788 1213 98-99 -0.14047619047619975 1213 100-101 3.890972222222228 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 8.0 19 7.0 20 16.0 21 26.0 22 28.0 23 32.0 24 39.0 25 43.0 26 52.0 27 41.0 28 67.0 29 73.0 30 70.0 31 94.0 32 79.0 33 86.0 34 96.0 35 103.0 36 150.0 37 179.0 38 85.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.89090909090909 20.872727272727275 11.418181818181818 25.818181818181817 2 15.429403202328967 20.451237263464336 40.174672489082965 23.94468704512373 3 18.69090909090909 24.87272727272727 34.836363636363636 21.6 4 12.072727272727272 15.127272727272729 43.56363636363636 29.236363636363638 5 12.727272727272727 31.490909090909092 40.50909090909091 15.272727272727273 6 26.10909090909091 37.67272727272727 21.09090909090909 15.127272727272729 7 22.472727272727273 30.4 28.654545454545456 18.472727272727273 8 23.127272727272725 36.43636363636364 23.854545454545452 16.581818181818182 9 21.672727272727276 18.472727272727273 24.363636363636363 35.49090909090909 10-11 24.509090909090908 23.745454545454546 31.6 20.145454545454545 12-13 21.854545454545455 23.418181818181818 32.76363636363636 21.96363636363636 14-15 19.963636363636365 24.981818181818184 27.636363636363637 27.418181818181818 16-17 19.89090909090909 29.63636363636364 29.163636363636364 21.30909090909091 18-19 19.963636363636365 29.563636363636363 30.0 20.472727272727273 20-21 20.909090909090907 29.2 27.527272727272727 22.363636363636363 22-23 20.618181818181817 27.96363636363636 30.327272727272724 21.09090909090909 24-25 19.272727272727273 29.63636363636364 29.563636363636363 21.527272727272727 26-27 19.636363636363637 28.03636363636364 29.272727272727273 23.054545454545455 28-29 20.50909090909091 28.836363636363636 29.63636363636364 21.01818181818182 30-31 19.345454545454547 29.236363636363638 29.927272727272726 21.490909090909092 32-33 21.96363636363636 26.654545454545453 30.0 21.381818181818183 34-35 21.12727272727273 28.836363636363636 28.327272727272728 21.709090909090907 36-37 20.0 28.581818181818182 30.0 21.418181818181818 38-39 21.454545454545453 27.636363636363637 30.363636363636363 20.545454545454543 40-41 20.472727272727273 29.454545454545457 29.599999999999998 20.472727272727273 42-43 19.163636363636364 26.727272727272727 31.963636363636365 22.145454545454545 44-45 18.836363636363636 26.036363636363635 32.0 23.127272727272725 46-47 18.763636363636362 29.2 30.581818181818182 21.454545454545453 48-49 19.818181818181817 27.78181818181818 29.745454545454546 22.654545454545456 50-51 20.072727272727274 28.145454545454545 31.454545454545457 20.327272727272728 52-53 20.4 27.854545454545455 29.78181818181818 21.96363636363636 54-55 18.945454545454545 28.581818181818182 29.345454545454547 23.127272727272725 56-57 20.4 29.309090909090905 30.254545454545458 20.036363636363635 58-59 18.836363636363636 28.654545454545456 30.363636363636363 22.145454545454545 60-61 19.527272727272727 30.763636363636365 28.763636363636362 20.945454545454545 62-63 18.4 29.12727272727273 30.03636363636364 22.436363636363637 64-65 19.2 27.81818181818182 30.8 22.181818181818183 66-67 19.78181818181818 28.61818181818182 29.854545454545455 21.745454545454546 68-69 20.18181818181818 29.563636363636363 29.381818181818183 20.872727272727275 70-71 19.454545454545453 31.563636363636366 28.109090909090913 20.872727272727275 72-73 18.327272727272728 29.09090909090909 30.181818181818183 22.400000000000002 74-75 18.90909090909091 30.072727272727274 27.563636363636363 23.454545454545457 76-77 19.490909090909092 30.545454545454547 27.527272727272727 22.436363636363637 78-79 18.509090909090908 30.872727272727275 28.61818181818182 22.0 80-81 19.854545454545455 30.4 29.2 20.545454545454543 82-83 19.818181818181817 29.599999999999998 28.145454545454545 22.436363636363637 84-85 18.254545454545454 30.363636363636363 29.599999999999998 21.78181818181818 86-87 18.69090909090909 30.581818181818182 29.672727272727272 21.054545454545455 88-89 20.0 31.127272727272725 27.854545454545455 21.01818181818182 90-91 18.69090909090909 29.599999999999998 29.927272727272726 21.78181818181818 92-93 18.8 29.563636363636363 30.727272727272727 20.909090909090907 94-95 20.21818181818182 28.87272727272727 28.03636363636364 22.87272727272727 96-97 18.327272727272728 30.836363636363632 29.309090909090905 21.527272727272727 98-99 18.036363636363635 30.727272727272727 29.89090909090909 21.345454545454544 100-101 19.927272727272726 28.654545454545456 29.963636363636365 21.454545454545453 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 1.0 18 1.5 19 2.5 20 2.5 21 1.0 22 5.5 23 10.5 24 13.5 25 16.5 26 22.5 27 26.5 28 24.5 29 28.5 30 33.0 31 39.5 32 46.5 33 40.5 34 41.5 35 51.5 36 49.5 37 45.5 38 48.5 39 46.0 40 44.0 41 48.5 42 53.5 43 59.0 44 51.0 45 45.5 46 49.0 47 41.5 48 32.0 49 26.5 50 23.5 51 22.0 52 25.5 53 29.5 54 27.0 55 23.0 56 21.5 57 20.5 58 17.5 59 12.0 60 8.5 61 11.5 62 12.5 63 10.0 64 9.0 65 9.0 66 7.5 67 5.5 68 5.0 69 6.0 70 7.5 71 5.0 72 2.5 73 2.5 74 1.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.07272727272727272 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1375.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.70909090909092 #Duplication Level Percentage of deduplicated Percentage of total 1 99.70824215900802 99.41818181818182 2 0.29175784099197666 0.5818181818181818 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTAAAAGAGTCTACCAGTAAAAATTCAAAAATCACGCGAGCACCAATGT 2 0.14545454545454545 No Hit CAATCTATTTTTCGTGTAACAATCTATTTTTTCGTGTAACAATCTATTTT 2 0.14545454545454545 No Hit CATGTATGTCAGTTTGGACAGCGTGAATGTTTTAAGACAGTCCAGCTTTT 2 0.14545454545454545 No Hit AAGCAAGCTTTTCGCCACCGATGATACAAGGCGTTCGCCAGTACCTTAGG 2 0.14545454545454545 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-11 0.0 0.0 0.0 12-13 0.0 0.0 0.0 14-15 0.0 0.0 0.0 16-17 0.0 0.0 0.0 18-19 0.0 0.0 0.0 20-21 0.0 0.0 0.0 22-23 0.0 0.0 0.0 24-25 0.0 0.0 0.0 26-27 0.0 0.0 0.0 28-29 0.0 0.0 0.10909090909090909 30-31 0.0 0.0 0.14545454545454545 32-33 0.0 0.0 0.21818181818181817 34-35 0.0 0.0 0.2545454545454545 36-37 0.0 0.0 0.43636363636363634 38-39 0.0 0.0 0.7636363636363637 40-41 0.0 0.0 1.4545454545454546 42-43 0.0 0.0 1.7818181818181817 44-45 0.0 0.0 2.0727272727272728 46-47 0.0 0.0 2.690909090909091 48-49 0.0 0.0 3.745454545454545 50-51 0.0 0.0 4.327272727272727 52-53 0.0 0.0 4.654545454545454 54-55 0.0 0.0 5.454545454545455 56-57 0.0 0.0 5.781818181818181 58-59 0.0 0.0 6.4 60-61 0.0 0.0 7.018181818181818 62-63 0.0 0.0 7.381818181818182 64-65 0.0 0.0 7.8545454545454545 66-67 0.0 0.0 8.181818181818182 68-69 0.0 0.0 8.327272727272728 70-71 0.0 0.0 8.8 72-73 0.0 0.0 9.163636363636364 74-75 0.0 0.0 9.418181818181818 76-77 0.0 0.0 9.963636363636363 78-79 0.0 0.0 10.472727272727273 80-81 0.0 0.0 10.8 82-83 0.0 0.0 11.163636363636364 84-85 0.0 0.0 11.381818181818183 86-87 0.0 0.0 11.600000000000001 88-89 0.0 0.0 11.781818181818181 >>END_MODULE >>Kmer Content pass >>END_MODULE