FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L3_GGACTCCT-TATCCTCT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L3_GGACTCCT-TATCCTCT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435443
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGCT550.002578805234.5429089
ATAGGGC804.4910458E-429.6853083
GTACAGC650.005842242429.2286136
CTAGGGC650.005842242429.2286134
TACACCG1205.374688E-627.7062915
ACCACGC700.00838426327.1439728
TAGCACT1056.8996094E-527.1408564
CTAAGAG700.00838896327.1408544
GTACACG700.00839836927.134621
CTACACC1806.679329E-926.3869444
ATACCGC908.9424313E-426.3869446
CCTACAC1457.8737503E-726.2049623
AGTACCG950.001225857324.9981585
GTCTAGT950.001227500324.9924141
GGTCTAT950.001227500324.9924141
TACCATG1601.8544742E-623.7509777
CATGGAC1201.7092931E-423.748253
TAAGGAG1000.001652710523.748255
TCTTAGG1401.7772027E-523.7482472
TAACACC1401.7772027E-523.7482474
Produced by FastQC (version 0.11.2)