FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L3_GGACTCCT-TATCCTCT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L3_GGACTCCT-TATCCTCT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435443
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT851.6299518E-533.529218
GTATAAG1001.29215E-633.265081
TATACTA1151.0339318E-733.0432785
TAAGGCA1201.5022488E-731.6664734
GGACAGG753.075441E-431.6664736
GCTGTAC600.003948397531.6664738
ACTGCGG1102.7284386E-630.227098
GTATTAG1303.0175397E-729.2440261
GTGTAAG1303.0175397E-729.2440261
CAGCGTA650.005840310429.230599
GGACCGA650.005840310429.230596
GTACTAC1503.312016E-828.5129221
AGCACCG1056.896434E-527.1426935
GCTACAC1606.22349E-826.7185883
GGATAAC1109.434243E-525.9208411
GTGCTAC1109.434243E-525.9208411
GTCTTAC1109.434243E-525.9208411
GCTGTCC1301.0002901E-525.5767678
CTATCCC950.001225373924.9998514
ACACAGG1151.280019E-424.782466
Produced by FastQC (version 0.11.2)