FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L36_AAGAGGCA-TATCCTCT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L36_AAGAGGCA-TATCCTCT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901022
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC1503.342575E-828.4932383
CTACACT3350.028.3514824
ACCGCTC2651.8189894E-1225.0911738
CTATACT2651.8189894E-1225.0883854
CTTAGTA1351.3447783E-524.6237892
GTATTAG2751.8189894E-1224.1733991
CTTACAC3000.023.7443663
TATACTC3050.023.3551165
AGTACCG2303.6343408E-922.7120025
CTGTCCC2103.448622E-822.6161929
GTATAGA2554.9294613E-1022.3451581
TACTTAG1302.943335E-421.9203117
TTATACT4400.021.5857874
TACACTG4850.021.5412835
TGCTAGG1353.8011494E-421.1061042
CTAACAC2551.1941665E-820.4853363
GTTAGCC1404.8583857E-420.3523123
GTGTAGC2601.4950274E-820.0891571
TAACCCG1200.004769255419.786975
TAGGACA2401.415865E-719.786974
Produced by FastQC (version 0.11.2)