FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L36_AAGAGGCA-TATCCTCT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L36_AAGAGGCA-TATCCTCT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901022
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAC2207.6397555E-1125.9232041
GTCCTAT1551.4033994E-624.5294841
GTATATG3350.024.1176071
TAGAGTG2252.8103386E-923.221985
GTGCTAG1852.5270674E-723.1206951
GTCCTAA1652.417446E-623.0428491
AGGACCG1452.3338082E-522.9307945
CTGTGTA2103.4444383E-822.618819
TAGGAGG1503.032522E-522.1664334
AGGGTCG1100.002877064321.5906835
CTAGGGA1100.002877064321.5906834
TATACAG3750.021.5331085
GTATAGA2709.931682E-1021.122611
CTAGATG1805.1671777E-621.1108884
GTGTACA4600.020.6634241
GTGTAGG3252.1827873E-1120.4726831
TCTAGGG1404.8418914E-420.362582
CTAGGAG1404.8455177E-420.3603173
GTACTAG1656.294347E-520.1624931
CAGGACT3302.7284841E-1120.1513024
Produced by FastQC (version 0.11.2)