FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L34_CGAGGCTG-TATCCTCT_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L34_CGAGGCTG-TATCCTCT_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504970
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAC751.3649878E-744.3159073
CCGGTAC550.002580727734.5387159
CTGTCCC1252.153538E-730.3940729
CTGTGCG1153.864787E-628.9074049
GTACAGG856.416898E-427.9301931
ATAGACC1205.3902913E-627.6974413
TAGGAGA1205.3902913E-627.6974414
ACCGCGG700.00839517127.1375668
GTATAGA1955.366019E-1026.7843381
CTAGTGC908.961115E-426.3785154
ACTGCTC2007.166818E-1026.1199058
CTGGACT1301.00371235E-525.5668684
TACACCG1701.12449925E-725.1371755
GCACTAC1151.2837842E-424.7728651
TCTAGGA1951.5719706E-824.3493982
CTCTCGC1201.7112259E-423.7453679
ATAGGTC1601.8624742E-623.7406653
TAGCACT1601.8624742E-623.7406654
CCTAGCA1000.001656151123.7406642
GTACTAG1201.7135269E-423.7406641
Produced by FastQC (version 0.11.2)