FastQCFastQC Report
Wed 17 Sep 2014
G140_A6_L34_CGAGGCTG-TATCCTCT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140_A6_L34_CGAGGCTG-TATCCTCT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504970
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAG554.9538205E-543.189972
TACGCCC550.0025783834.545134
GTTAAGG902.4088271E-531.6695063
TAAGAGT1509.531504E-1031.6663724
GTATAAT3650.027.3447551
TGGGTCG700.00838757627.1426035
ACAGGCC700.00838757627.1426038
TACAGGC700.00838757627.1426037
GTATATG1606.192022E-826.7343851
TACGGGT1109.466917E-525.908854
CAAGGTC1109.466917E-525.908854
ACCGTGT1109.466917E-525.908858
CTGTTCG1301.0007292E-525.5766819
CTGGACC1301.0007292E-525.5766814
GGGGTGT950.001225669424.9997656
TATACCG1151.2804443E-424.7823775
TACCGCC1151.2804443E-424.7823777
ACTGTCC1351.3408868E-524.6293988
TTATACC1551.4081361E-624.5158984
GAGTGCA1951.5654223E-824.3587479
Produced by FastQC (version 0.11.2)